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1.
Tsybin YO Witt M Baykut G Håkansson P 《Rapid communications in mass spectrometry : RCM》2004,18(14):1607-1613
Electron capture dissociation (ECD) of polypeptide cations was obtained with pencil and hollow electron beams for both sidekick and gas-assisted dynamic ion trapping (GADT) using Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) with an electrostatic ion transfer line. Increasing the number of trapped ions by multiple ICR trap loads using GADT improved the ECD sensitivity in comparison with sidekick ion trapping and ECD efficiency in comparison with single ion trap load by GADT. Furthermore, enhanced sensitivity made it possible to observe ECD in a wide range of electron energies (0-50 eV). The degree, rate and fragmentation characteristics of ECD FTICR-MS were investigated as functions of electron energy, electron irradiation time, electron flux and ion trapping parameters for this broad energy range. The results obtained show that the rate of ECD is higher for more energetic (>1 eV) electrons. Long electron irradiation time with energetic electrons reduces average fragment ion mass and decreases efficiency of formation of c- and z-type ions. The obtained dependencies suggest that the average fragment ion mass and the ECD efficiency are functions of the total fluence of the electron beam (electron energy multiplied by irradiation time). The measured electron energy distributions in low-energy ECD and hot ECD regimes are about 1 eV at full width half maximum in employed experimental configurations. 相似文献
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Meier SM Tsybin YO Dyson PJ Keppler BK Hartinger CG 《Analytical and bioanalytical chemistry》2012,402(8):2655-2662
The interaction between oxaliplatin and the model protein ubiquitin (Ub) was investigated in a top–down approach by means
of high-resolution electrospray ionization mass spectrometry (ESI-MS) using diverse tandem mass spectrometric (MS/MS) techniques,
including collision-induced dissociation (CID), higher-energy C-trap dissociation (HCD), and electron transfer dissociation
(ETD). To the best of our knowledge, this is the first time that metallodrug–protein adducts were analyzed for the metal-binding
site by ETD-MS/MS, which outperformed both CID and HCD in terms of number of identified metallated peptide fragments in the
mass spectra and the localization of the binding sites. Only ETD allowed the simultaneous and exact determination of Met1
and His68 residues as binding partners for oxaliplatin. CID-MS/MS experiments were carried out on orbitrap and ion cyclotron
resonance (ICR)-FT mass spectrometers and both instruments yielded similar results with respect to number of metallated fragments
and the localization of the binding sites. A comparison of the protein secondary structure with the intensities of peptide
fragments generated by collisional activation of the [Ub + Pt-(chxn)] adduct [chxn = (1R,2R)-cyclohexanediamine] revealed a correlation with cleavages in solution phase random coil areas, indicating that the N-terminal β-hairpin and α-helix structures are retained in the gas phase. 相似文献
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M. N. Drozdov E. B. Kluenkov A. Ya. Lopatin V. I. Luchin A. E. Pestov N. N. Salashchenko N. N. Tsybin N. I. Chkhalo L. A. Shmaenok 《Bulletin of the Russian Academy of Sciences: Physics》2011,75(1):73-75
New Mo-based multilayer structures are proposed, and sample free-standing filters 160 mm in diameter with a transparency of
71% at a wavelength of λ = 13.5 nm are described. The enhanced thermo-oxidative stability of filters with Mo layers is demonstrated
as opposed to Zr-based films. It is shown in particular that Mo-based filters can withstand vacuum heat annealing at 800°C
for three hours without any optical and structural changes. 相似文献
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Wågberg T Johnels D Peera A Hedenström M Schulga YM Tsybin YO Purcell JM Marshall AG Noreus D Sato T Talyzin AV 《Organic letters》2005,7(25):5557-5560
[chemical structure: see text]. C60H18 has been produced by hydrogenation of C60 at 100 bar H2 pressure and 673 K for 10 h. We have investigated the crude material without any purification by use of 1H NMR, 13C NMR, and IR spectroscopy and Fourier transform ion cyclotron resonance mass spectrometry. We show that the crude material consists of 95% of the C3v isomer of C60H18. 相似文献
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Vincent Larraillet Aleksey Vorobyev Claire Brunet Jér?me Lemoine Yury O. Tsybin Rodolphe Antoine Philippe Dugourd 《Journal of the American Society for Mass Spectrometry》2010,21(4):670-680
We compare product-ion mass spectra produced by electron detachment dissociation (EDD) and electron photodetachment dissociation
(EPD) of multi-deprotonated peptides on a Fourier transform and a linear ion trap mass spectrometer, respectively. Both methods,
EDD and EPD, involve the electron emission-induced formation of a radical oxidized species from a multi-deprotonated precursor
peptide. Product-ion mass spectra display mainly fragment ions resulting from backbone cleavages of Cα-C bond ruptures yielding a and x ions. Fragment ions originating from N-Cα backbone bond cleavages are also observed, in particular by EPD. Although EDD and EPD methods involve the generation of a
charge-reduced radical anion intermediate by electron emission, the product ion abundance distributions are drastically different.
Both processes seem to be triggered by the location and the recombination of radicals (both neutral and cation radicals).
Therefore, EPD product ions are predominantly formed near tryptophan and histidine residues, whereas in EDD the negative charge
solvation sites on the backbone seem to be the most favorable for the nearby bond dissociation. 相似文献
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Akhsakhalyan A. A. Gavrilin D. A. Malyshev I. V. Salashchenko N. N. Toropov M. N. Ulasevich B. A. Tsybin N. N. Chkhalo N. I. 《Technical Physics》2019,64(11):1698-1703
Technical Physics - An experimental setup and results on aberration of sources of a reference spherical wave (SRSW) based on a single-mode optical fiber with a subwavelength output aperture... 相似文献
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L. Ya. Grona V. M. Gul'ko N. F. Kolomiets A. S. Tsybin A. E. Shikanov K. I. Yakovlev 《Russian Physics Journal》1990,33(4):333-335
Effects on deuteron emission stability have been examined in relation to metal deuteride formation in plasma targets for laser ion sources. Erbium and zirconium deuterides have been used to demonstrate the factors that increase the emission stability by substantial amounts.Translated from Izvestiya Vysshikh Uchebnykh Zavedenii, Fizika, No. 4, pp. 60–64, April, 1990. 相似文献
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M. N. Drozdov E. B. Kluenkov A. Ya. Lopatin V. I. Luchin A. E. Pestov N. N. Salashchenko N. N. Tsybin N. I. Chkhalo L. A. Shmaenok 《Bulletin of the Russian Academy of Sciences: Physics》2013,77(1):83-85
The thermal stability of multilayer thin-film Mo/ZrSi2 and Mo/NbSi2 filters in the temperature range of 840–940°C upon long-term heating in vacuum is investigated. The filter transmittance is measured before and after current annealing and a layer-by-layer SIMS analysis of the structure is performed. Comparative testing shows that the Mo/NbSi2 structure is less thermostable at high thermal loads. 相似文献