排序方式: 共有8条查询结果,搜索用时 15 毫秒
1
1.
Cocaine is transformed into hepatotoxic metabolites through oxidative pathways. For anhydroecgonine methyl ester (AEME), the main constituent in crack smoke, the oxidative metabolism has not been studied. Therefore, incubation of AEME with rat liver microsomes was performed and a metabolite of AEME, anhydroecgonine methyl ester N-oxide (AEMENO), was identified. The chemical structure of this new metabolite was confirmed by synthesis and by comparative interpretation of electrospray multiple-stage mass spectra, which were obtained in the positive ion mode. This metabolite was also detected in whole blood, serum and urine samples from crack users. The application of liquid chromatography/electrospray mass spectrometry or nanoelectrospray mass spectrometry was necessary because AEMENO is susceptible to thermal degradation during gas chromatographic/mass spectrometric analysis. This study demonstrated that AEMENO is produced by rat hepatic microsomal metabolism in vitro and is present in body fluids from crack users. 相似文献
2.
3.
Toennes SW Schneider K Kauert GF Wunder C Moeller MR Theunissen EL Ramaekers JG 《Analytical and bioanalytical chemistry》2011,400(1):145-152
Cannabis is not only the most widely used illicit drug worldwide but is also regularly consumed along with ethanol. In previous
studies, it was assumed that cannabis users develop cross-tolerance to ethanol effects. The present study was designed to
compare the effects of ethanol in comparison to and in combination with a cannabis joint and investigate changes in pharmacokinetics.
In this study, 19 heavy cannabis users participated and received three alcohol dosing conditions that were calculated to achieve
steady blood alcohol concentrations (BAC) of about 0, 0.5 and 0.7 g/l during a 5-h time window. Subjects smoked a Δ9-tetrahydrocannabinol (THC) cigarette (400 μg/kg) 3 h post-onset of alcohol dosing. Blood samples were taken between 0 and
4 h after smoking. During the first hour, samples were collected every 15 min and every 30 min thereafter. Mean steady-state
BACs reached 0, 0.36 and 0.5 g/l. The apparent elimination half-life of THC was slightly prolonged (1.59 vs. 1.93 h, p < 0.05) and the concentration 1 h after smoking was slightly lower (24 vs. 17 ng/ml, p < 0.05) with the higher ethanol dose. The prolonged THC elimination might be explained by a small ethanol-mediated change
in distribution to and from deep compartments. Concentrations and pharmacokinetics of 11-hydroxy-THC and 11-nor-9-carboxy-THC
(THCA) were not significantly influenced by ethanol. However, THCA concentrations appeared lower in both ethanol conditions,
which might also be attributable to changes in distribution. Though not significant in the present study, this might be relevant
in the interpretation of cannabinoid concentrations in blood. 相似文献
4.
5.
Stefan W. Toennes Moritz G. Wagner Gerold F. Kauert 《Analytical and bioanalytical chemistry》2010,398(2):769-777
Acetaldehyde adducts of hemoglobin have been regarded as potential biochemical markers of alcohol exposure. In this study
a novel sensitive method using liquid chromatography coupled to time-of-flight mass spectrometry (LC–TOF MS) has been used
to investigate changes in adduct levels in alcohol detoxification patients. Hemoglobin and authentic blood samples from 66
adults with an alcohol-dependence syndrome and from 12 children were analyzed for acetaldehyde modifications with and without
trypsin digestion using LC–TOF MS. After in-vitro incubation of hemoglobin with increasing concentrations of acetaldehyde,
followed by tryptic digestion, 21 modified peptide fragments could be identified from their accurate mass and retention time
shift. Eight of these could also be detected in authentic human blood samples. Trace amounts in children’s blood were indicative
of an endogenous source. Modified peptide levels in patients’ samples with and without ethanol were significantly different,
as also were levels in samples from admission and from five days later. Samples obtained 5, 10, or 15 days after admission
did not differ in adduct levels. The LC–TOF MS method was sensitive enough to detect acetaldehyde-modified hemoglobin peptides
in blood samples from patients with an alcohol-dependence syndrome. However, elevated levels were only observed after recent
ethanol consumption and decreased during five days of abstinence, suggesting that acetaldehyde-modified tryptic peptides of
hemoglobin are potential biomarkers only for short-term ethanol ingestion. 相似文献
6.
Methyl-methine linkages of Novolac, a commercially available t-butylphenol acetylene condensed (TBPA) resin, have been identified by recognition of pyrolysis pathways using pyrolysis-gas chromatography/mass spectrometry (Py-GC/mS) in vulcanized rubber. The diagnostic mass spectrum of t-butylphenol with methyl-methine linkages between phenolic rings was observed at m/z 192, corresponding to 4-t-butyl-2-ethyl-6-methylphenol. Other molecular ions were observed at m/z 178, 164, and 150 in the characteristic pyrolyzates. The ion at m/z 192 in the TBPA resin was observed to be characteristic for methyl-methine linkages between the phenolic groups, and the analytical pyrolysis-GC/mS method was thus able to identify the resin at low levels in vulcanized rubber. Copyright 1999 John Wiley & Sons, Ltd. 相似文献
7.
8.
The ergot alkaloid lysergic acid amide (LSA) is a secondary plant constituent in a number of plants, but it is mainly present in considerable amounts in Convolvulaceae, like Argyreia nervosa. Due to its close structural similarity to lysergic acid diethylamide, LSA is considered as psychedelic and therefore promoted as so-called “legal high” in various internet forums. During a human behavioral study with orally administered seeds of A. nervosa, blood and urine samples were obtained. The present study describes the validation of a sensitive and robust high performance liquid chromatography method with fluorescence detection, which was applied to the study samples. The limit of detection (LOD) and lower limit of quantification in human serum were 0.05 and 0.17 ng/mL, respectively, and in urine, the LOD was 0.15 ng/mL. Intra- and interday precision and accuracy were below 15 % relative standard deviation with a bias better than ±15 %. No conversion of LSA to its epimer iso-LSA was noted during analyses. The LSA concentrations in the authentic human serum samples were in the range of 0.66 to 3.15 ng/mL approximately 2 h after ingestion. In urine, LSA could be found 1–24 h after ingestion; after 48 h, no LSA could be detected. The LSA epimer iso-LSA was also detected in serum and urine in varying ratios. In conclusion, LSA serum levels in the low nanogram per milliliter range correlated with severe vegetative adverse effects (nausea, weakness, fatigue, tremor, blood pressure elevation) and a psychosis-like state, which led to study termination. 相似文献
1