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I. T. L. Bresolin M. Borsoi-Ribeiro W. M. S. C. Tamashiro E. F. P. Augusto M. A. Vijayalakshmi S. M. A. Bueno 《Applied biochemistry and biotechnology》2010,160(7):2148-2165
Monoclonal antibodies (MAbs) have been used for therapies and some analytical procedures as highly purified molecules. Many
techniques have been applied and studied, focusing on monoclonal antibodies purification. In this study, an immobilized metal
affinity chromatography membrane was developed and evaluated for the purification of anti-TNP IgG1 mouse MAbs from cell culture supernatant after precipitation with a 50% saturated ammonium sulfate solution. The chelating
ligands iminodiacetic acid, carboxymethylated aspartic acid (CM-Asp), nitrilotriacetic acid, and tris (carboxymethyl) ethylenediamine
in agarose gels with immobilized Ni(II) and Zn(II) ions were compared for the adsorption and desorption of MAbs. The most
promising chelating ligand—CM-Asp—was then coupled to poly(ethylene vinyl alcohol) (PEVA) hollow fiber membranes. According
to SDS-PAGE and ELISA analyses, a higher selectivity and a purification factor of 85.9 (fraction eluted at 500 mM Tris) were
obtained for IgG1 using PEVA-CM-Asp-Zn(II). The anti-TNP MAb could be eluted under mild pH conditions causing no loss of antigen binding capacity. 相似文献
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Ruoyu Hong * Jianmin Ding Guoliang Zheng College of Chemistry Chemical Engineering Soochow University Suzhou P. R. China Institute of Process Engineering Chinese Academy of Sciences Beijing P. R. China IBM HYDA/- C Highway North Rochester MN USA *Author to whom correspondence should be addressed. E-mail: rhong@suda.edu.cn 《中国颗粒学报》2004,2(5):207-214
1. Introduction1.1 Silica nanoparticles and synthesis methods Silica (SiO2) nanoparticles are widely used in industry asan active filler for polymer reinforcement, a rheologicaladditive in fluids, a free flow agent in powders, and anagent for chemical mechanical polishing during IC (inte-grated circuit) fabrication (Sniegowski & de Boer, 2000).Silica powder is also used for producing silicon carbide(Koc & Cattamanchi, 1998) or opaque silica aerosols (Leeet al., 1995). Many methods can … 相似文献
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In this paper, we consider nonlinear Schrödinger (NLS) equations, both in the anomalous and normal dispersive regimes, which govern the propagation of a single field in a fiber medium with phase modulation and fibre gain (or loss). The integrability conditions are arrived from linear eigen value problem. The variable transformations which connect the integrable form of modified NLS equations are presented. We succeed in Hirota bilinearzing the equations and on solving, exact bright and dark soliton solutions are obtained. From the results, we show that the soliton is alive, i.e. pulse area can be conserved by the inclusion of gain (or loss) and phase modulation effects. 相似文献
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JIANG DiHua NIEN ChuFeng & QIN YuJun School of Mathematics University of Minnesota Minneapolis MN USA 《中国科学 数学(英文版)》2010,(3)
In this paper, we summarize the basic structures and properties of irreducible symplectic supercuspidal representations of GLn(F) over a p-adic local field F with characteristic zero, and explore possible topics for further investigation. 相似文献
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Tamashiro MN Henriques VB Lamy MT 《Langmuir : the ACS journal of surfaces and colloids》2005,21(24):11005-11016
We theoretically investigate the dependence of the surface charge developed on charged spherical colloids upon several environmental parameters: the ionic strength of the monovalent added electrolyte, acidity (stabilized by a pH buffer solution), and colloid concentration. In the framework of the mean-field Poisson-Boltzmann spherical cell model, we include the charged colloid-microion correlations into the buffer equation, and we allow for the specific binding of ions to the ionizable groups on the colloid surface. Theoretical predictions are compared to the results obtained under the planar-symmetry Gouy-Chapman approximation and analyzed for the experimental conditions of an aqueous dispersion of the phospholipid dimyristoyl phosphatidylglycerol (DMPG). Experimental measurements of the partition ratio of an aqueous soluble cationic spin label on buffered dispersions of polyanionic unilamellar vesicles of DMPG in the presence of added monovalent salt are theoretically interpreted in terms of ion partition due to electrostatic interactions. We show that the specific binding of the probe must be admitted to explain the experimental results. 相似文献
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Obliquely propagating altra-low-frequency dust-electromagnetic waves in a self-gravitating, warm, magnetized, two fluid dusty
plasma system have been investigated. Two special cases, namely, dust-Alfvén mode propagating parallel to the external magnetic
field and dustmagnetosonic mode propagating perpendicular to the external magnetic field have also been considered. It has
been shown that effects of self-gravitational field, dust fluid temperature, and obliqueness significantly modify the dispersion
properties of these ultra-low-frequency dust-electromagnetic modes. It is also found that in parallel propagating dust-Alfvén
mode these effects play no role, but in obliquely propagating dust-Alfvén mode or perpendicular propagating dust-magnetosonic
mode the effect of self-gravitational field plays destabilizing role whereas the effect of dust/ion fluid temperature plays
stabilizing role. 相似文献
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Gene A. Hiegel Christopher D. Bayne Yariv Donde Gerald S. Tamashiro Lisa A. Hilberath 《合成通讯》2013,43(14):2633-2639
Aldehydes are readily oxidized to methyl esters by a solution of methanol, pyridine, and trichloroisocyanuric acid in acetonitrile, acetone, or methylene chloride. 相似文献
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Loren?S?Scuro PU?Simioni DL?Grabriel Elzira?E?Saviani Luzia?V?Modolo Wirla?MSC?Tamashiro Ione?SalgadoEmail author 《BMC biochemistry》2004,5(1):5
Background
The anti-inflammatory properties of some flavonoids have been attributed to their ability to inhibit the production of NO by activated macrophages. Soybean cotyledons accumulate certain flavonoids following elicitation with an extract of the fungal pathogen Diaporthe phaseolorum f. sp. meridionalis (Dpm). Sodium nitroprusside (SNP), a nitric oxide donor, can substitute for Dpm in inducing flavonoid production. In this study, we investigated the effect of flavonoid-containing diffusates obtained from Dpm- and SNP-elicited soybean cotyledons on NO production by lipopolysaccharide (LPS)- and LPS plus interferon-γ (IFNγ)-activated murine macrophages.Results
Significant inhibition of NO production, measured as nitrite formation, was observed when macrophages were activated in the presence of soybean diffusates from Dpm- or SNP-elicited cotyledons. This inhibition was dependent on the duration of exposure to the elicitor. Daidzein, genistein, luteolin and apigenin, the main flavonoids present in diffusates of elicited cotyledons, suppressed the NO production by LPS + IFNγ activated macrophages in a concentration-dependent manner, with IC50 values of 81.4 μM, 34.5 μM, 38.6 μM and 10.4 μM respectively. For macrophages activated with LPS alone, the IC50 values were 40.0 μM, 16.6 μM, 10.4 μM and 2.8 μM, respectively. Western blot analysis showed that iNOS expression was not affected by daidzein, was reduced by genistein, and was abolished by apigenin, luteolin and Dpm- and SNP-soybean diffusates at concentrations that significantly inhibited NO production by activated macrophages.Conclusions
These results suggest that the suppressive effect of flavonoids on iNOS expression could account for the potent inhibitory effect of Dpm- and SNP-diffusates on NO production by activated macrophages. Since the physiological concentration of flavonoids in plants is normally low, the treatment of soybean tissues with SNP may provide a simple method for substantially increasing the concentration of metabolites that are beneficial for the treatment of chronic inflammatory diseases associated with NO production.10.
Henriques VB Germano R Lamy MT Tamashiro MN 《Langmuir : the ACS journal of surfaces and colloids》2011,27(21):13130-13143
Aqueous dispersions of phosphatidylglycerol (PG) lipids may present an anomalous chain-melting transition at low ionic strengths, as seen by different experimental techniques such as calorimetry or light scattering. The anomaly disappears at high ionic strengths or for longer acyl-chain lengths. In this article, we use a statistical model for the bilayer that distinguishes both lipid chain and headgroup states in order to compare model and experimental thermotropic and electrical properties. The effective van der Waals interactions among hydrophobic chains compete with the electrostatic repulsions between polar headgroups, which may be ionized (counterion dissociated) or electrically neutral (associated with counterions). Electric degrees of freedom introduce new thermotropic charge-ordered phases in which headgroup charges may be spatially ordered, depending on the electrolyte ionic strength, introducing a new rationale for experimental data on PGs. The thermal phases presented by the model for different chain lengths, at fixed ionic strength, compare well with an experimental phase diagram constructed on the basis of differential scanning calorimetry profiles. In the case of dispersions of DMPG (dimyristoyl phosphatidylglycerol) with added monovalent salt, the model properties reproduce the main features displayed by data from differential scanning calorimetry as well as the characteristic profile for the degree of ionization of the bilayer surface across the anomalous transition region, obtained from the theoretical interpretation of electrokinetic (conductivity and electrophoretic mobility) measurements. 相似文献
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