排序方式: 共有15条查询结果,搜索用时 0 毫秒
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Sanford TW Allshouse GO Marder BM Nash TJ Mock RC Spielman RB Seamen JF McGurn JS Jobe D Gilliland TL Vargas M Struve KW Stygar WA Douglas MR Matzen MK Hammer JH De Groot JS Eddleman JL Peterson DL Mosher D Whitney KG Thornhill JW Pulsifer PE Apruzese JP Maron Y 《Physical review letters》1996,77(25):5063-5066
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Anders Rygh Swensen 《Stochastic Processes and their Applications》1983,15(2):181-191
In this note some problems of asymptotic inference in a class of non-stationary stochastic processes are considered. In particular, it is shown that no criterion based on the existence of uniformly most powerful tests over a local neighborhood can be used in this situation. 相似文献
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An integral equation formulation for buoyancy-driven convection problems is developed and illustrated. Buoyancy-driven convection in a bounded cylindrical geometry with a free surface is studied for a range of aspect ratios and Nusselt numbers. The critical Rayleigh number, the nature of the cellular motion, and the heat transfer enhancement are computed using linear theory. Green's functions are used to convert the linear problem into linear Fredholm integral equations. Theorems are proved which establish the properties of the eigenvalues and eigenfunctions of the linear integral operator which appears in these equations. 相似文献
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Koech PK Polikarpov E Rainbolt JE Cosimbescu L Swensen JS Von Ruden AL Padmaperuma AB 《Organic letters》2010,12(23):5534-5537
We studied the influence of a pyridine moiety versus a phenyl moiety when introduced in the molecular design of an ambipolar host. These pyridine-based host materials for organic light-emitting diodes (OLEDs) were synthesized in three to five steps from commercially available starting materials. The isomeric hosts have similar HOMO/LUMO energies; however, data from OLEDs fabricated using the above host materials demonstrate that small structural modification of the host results in significant changes in its carrier-transporting characteristics. 相似文献
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Fernández P Durand JS Pérez-Conde C Paniagua G 《Analytical and bioanalytical chemistry》2003,375(8):1020-1023
This paper reports a new flow-through fluoroimmunosensor, the function of which is based on antibodies immobilized on an inmunoreactor of controlled-pore glass (CPG), for determination of digoxin, used in the treatment of congestive heart failure and artery disease. The immunosensor has a detection limit of 1.20 microg L(-1) and provides high reproducibility (RSD=4.5% for a concentration of 0.0025 mg L(-1), and RSD=6.7% for 0.01 mg L(-1)). The optimum working concentration range was found to be 1.2 x 10(-3)-4.0 x 10(-2) mg L(-1). The lifetime of the immunosensor was about 50 immunoassays; if stored unused its lifetime can be extended to three months. A sample speed of about 10-12 samples per hour can be attained. Possible interference from substances with structures similar to digoxin (morphine, heroin, tebaine, codeine, pentazocine and narcotine) was investigated. No cross-reactivity was seen at the highest digoxin: interferent ratio studied (1:100). The proposed fluoroimmunosensor was successfully used to determine digoxin concentrations in human serum samples. 相似文献
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Anders Rygh Swensen 《Stochastic Processes and their Applications》1985,19(1):111-123
Some optimal inference results for a class of diffusion processes, including the continuous state branching process and the approximate Wright-Fisher model with selection, are derived.It is then showed how the theory of convergence of experiments, due to Le Cam, can be applied to derive corresponding results for processes approximating these diffusions. 相似文献
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Mezler M Hermann D Swensen AM Draguhn A Terstappen GC Gross G Schoemaker H Freiberg G Pratt S Gopalakrishnan SM Nimmrich V 《Combinatorial chemistry & high throughput screening》2012,15(5):372-385
Dysfunction of P/Q-type calcium channels is thought to underlie a variety of neurological diseases. There is evidence that migraine, Alzheimer's disease, and epilepsy involve a gain-of-function of the channel, leading to abnormal presynaptic vesicle release. P/Q-channel blockers may normalize current flow and consequently lead to an alleviation of disease symptoms. Although the medical need is high, there are no such compounds on the market. Here we describe a high throughput screen (HTS) for P/Q-type calcium channel blockers and the confirmation of hits by automated electrophysiology. We generated a HEK293 cell line stably expressing the α1A subunit of the P/Q-type calcium channel under control of a tetracycline (Tet) promoter. The accessory β1.1 and α2δ1 subunits were co-expressed constitutively. The cell line was pharmacologically characterized by ion channel specific modulators, and revealed functional P/Q-type calcium currents. Using a fluorescence imaging plate reader (FLIPR), an assay for P/Q-type calcium channels was established based on a calcium sensitive dye. HTS of a 150,000 compound-containing sub-library led to the identification of 3262 hits that inhibited the fluorescence signal with potencies below 10 μM. Hit-to-lead (HTL) efforts identified 12,400 analogues. Compounds were clustered into 37 series, and 8 series of interest were prioritized. An electrophysiological secondary screen, providing a more direct measure of channel function, was implemented into the HTL process. 27 selected exemplars of different chemotypes were validated by automated whole-cell patch clamp analysis at inactivated channel state. The discovery of P/Q-channel blockers may foster the development of new therapeutics for a variety of neurological diseases. 相似文献