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Yen-Ting Lin Agnieszka Stańczak Yulian Manchev Dr. Grit D. Straganz Dr. Sam P. de Visser 《Chemistry (Weinheim an der Bergstrasse, Germany)》2020,26(10):2233-2242
Decarboxylation of fatty acids is an important reaction in cell metabolism, but also has potential in biotechnology for the biosynthesis of hydrocarbons as biofuels. The recently discovered nonheme iron decarboxylase UndA is involved in the biosynthesis of 1-undecene from dodecanoic acid and using X-ray crystallography was assigned to be a mononuclear iron species. However, the work was contradicted by spectroscopic studies that suggested UndA to be more likely a dinuclear iron system. To resolve this controversy we decided to pursue a computational study on the reaction mechanism of fatty acid decarboxylation by UndA using iron(III)-superoxo and diiron(IV)-dioxo models. We tested several models with different protonation states of active site residues. Overall, however, the calculations imply that mononuclear iron(III)-superoxo is a sluggish oxidant of hydrogen atom abstraction reactions in UndA and will not be able to activate fatty acid residues by decarboxylation at room temperature. By contrast, a diiron-dioxo complex reacts with much lower hydrogen atom abstraction barriers and hence is a more likely oxidant in UndA. 相似文献
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GD Fletcher 《Molecular physics》2013,111(23-24):2971-2976
A scalable multi-configuration self-consistent field (MCSCF) algorithm is described. The method for optimizing the orbital and configurational parameters is based upon the two-step Newton–Raphson approach with an augmented orbital Hessian matrix. A single copy of the two-electron integrals in the molecular orbital basis is distributed over the memory of all processors. Storage of the augmented Hessian is avoided by re-computing its elements as needed. A replicated data approach is used to parallelize the configuration interaction step. Scalability to 1024 processors is demonstrated. 相似文献
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Acetylacetone dioxygenase from Acinetobacter johnsonii (Dke1) utilizes a non-heme Fe2+ cofactor to promote dioxygen-dependent conversion of 2,4-pentanedione (PD) into methylglyoxal and acetate. An oxidative carbon-carbon bond cleavage by Dke1 is triggered from a C-3 peroxidate intermediate that performs an intramolecular nucleophilic attack on the adjacent carbonyl group. But how does Dke1 bring about the initial reduction of dioxygen? To answer this question, we report here a reaction coordinate analysis for the part of the Dke1 catalytic cycle that involves O2 chemistry. A weak visible absorption band (epsilon approximately 0.2 mM(-1) cm(-1)) that is characteristic of an enzyme-bound Fe2+-beta-keto-enolate complex served as spectroscopic probe of substrate binding and internal catalytic steps. Transient and steady-state kinetic studies reveal that O2-dependent conversion of the chromogenic binary complex is rate-limiting for the overall reaction. Linear free-energy relationship analysis, in which apparent turnover numbers (k(app) cat) for enzymatic bond cleavage of a series of substituted beta-dicarbonyl substrates were correlated with calculated energies for the highest occupied molecular orbitals of the corresponding beta-keto-enolate structures, demonstrates unambiguously that k(app) cat is governed by the electron-donating ability of the substrate. The case of 2'-hydroxyacetophenone (2'HAP), a completely inactive beta-dicarbonyl analogue that has the enol double bond delocalized into the aromatic ring, indicates that dioxygen reduction and C-O bond formation cannot be decoupled and therefore take place in one single kinetic step. 相似文献
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Straganz GD Hofer H Steiner W Nidetzky B 《Journal of the American Chemical Society》2004,126(39):12202-12203
Acinetobacter johnsonii acetylacetone dioxygenase (Dke1) is a non-heme Fe(II)-dependent dioxygenase that cleaves C-C bonds in various beta-dicarbonyl compounds capable of undergoing enolization to a cis-beta-keto enol structure. Results from 18O labeling experiments and quantitative structure-reactivity relationship analysis of electronic substituent effects on the substrate cleavage specificity of Dke1 are used to distinguish between two principle chemical mechanisms of reaction: one involving a 1,2-dioxetane intermediate and another proceeding via Criegee rearrangement. Oxygenative cleavage of asymmetrically substituted beta-dicarbonyl substrates occurs at the bond adjacent to the most electron-deficient carbonyl carbon. Replacement of the acetyl group in 1-phenyl-1,3-butanedione by a trifluoro-acetyl group leads to a complete reversal of cleavage frequency from 83% to only 8% fission of the bond next to the benzoyl moiety. The structure-activity correlation for Dke1 strongly suggests that enzymatic bond cleavage takes place via nucleophilic attack to generate a dioxetane, which then decomposes into the carboxylate and alpha-keto-aldehyde products. 相似文献
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Diebold AR Straganz GD Solomon EI 《Journal of the American Chemical Society》2011,133(40):15979-15991
The O(2) activating mononuclear nonheme iron enzymes generally have a common facial triad (two histidine and one carboxylate (Asp or Glu) residue) ligating Fe(II) at the active site. Exceptions to this motif have recently been identified in nonheme enzymes, including a 3His triad in the diketone cleaving dioxygenase Dke1. This enzyme is used to explore the role of the facial triad in directing reactivity. A combination of spectroscopic studies (UV-vis absorption, MCD, and resonance Raman) and DFT calculations is used to define the nature of the binding of the α-keto acid, 4-hydroxyphenlpyruvate (HPP), to the active site in Dke1 and the origin of the atypical cleavage (C2-C3 instead of C1-C2) pattern exhibited by this enzyme in the reaction of α-keto acids with dioxygen. The reduced charge of the 3His triad induces α-keto acid binding as the enolate dianion, rather than the keto monoanion, found for α-keto acid binding to the 2His/1 carboxylate facial triad enzymes. The mechanistic insight from the reactivity of Dke1 with the α-keto acid substrate is then extended to understand the reaction mechanism of this enzyme with its native substrate, acac. This study defines a key role for the 2His/1 carboxylate facial triad in α-keto acid-dependent mononuclear nonheme iron enzymes in stabilizing the bound α-keto acid as a monoanion for its decarboxylation to provide the two additional electrons required for O(2) activation. 相似文献
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