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Veeranarayanan S Poulose AC Mohamed S Aravind A Nagaoka Y Yoshida Y Maekawa T Kumar DS 《Journal of fluorescence》2012,22(2):537-548
The use of fluorescent nanomaterials has gained great importance in the field of medical imaging. Many traditional imaging
technologies have been reported utilizing dyes in the past. These methods face drawbacks due to non-specific accumulation
and photobleaching of dyes. We studied the uptake and internalization of two different sized (30 nm and 100 nm) FITC labeled
silica nanoparticles in Human umbilical vein endothelial cell line. These nanomaterials show high biocompatability and are
highly photostable inside live cells for increased period of time in comparison to the dye alone. To our knowledge, we report
for the first time the use of 30 nm fluorescent silica nanoparticles as efficient endothelial tags along with the well studied
100 nm particles. We also have emphasized the good photostability of these materials in live cells. 相似文献
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Mangathayaru Kalachaveedu Sunu Papacchan Sudip Sanyal Teena Koshy Srivani Telapolu 《Natural product research》2015,29(12):1118-1121
Major saponins of Brahmi (Bacopa monniera, Fam: Scrophulariaceae) – bacosides A and B – were isolated from the total methanol extract and characterised based on melting point, TLC, IR, 1H NMR and 13C NMR. They were evaluated for their in vitro cytogenetic effects on human peripheral blood lymphocytes by chromosomal aberration (CA) assay and sister chromatid exchange (SCE) assay. The frequency of chromatid type aberrations and reciprocal interchanges between sister chromatids in the treated cells was scored in comparison to the untreated control. At 30 μg/mL dose, bacoside A showed a statistically significant increase in the frequency of both CA and SCE and bacoside B showed an increase only in SCE. Our report of the genotoxicity of the saponins is significant in view of the reports of anticancer activity of Brahmi extracts. 相似文献
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Kulkarni RG Srivani P Achaiah G Sastry GN 《Journal of computer-aided molecular design》2007,21(4):155-166
The p38 protein kinase is a serine–threonine mitogen activated protein kinase, which plays an important role in inflammation
and arthritis. A combined study of 3D-QSAR and molecular docking has been undertaken to explore the structural insights of
pyrazolyl urea p38 kinase inhibitors. The 3D-QSAR studies involved comparative molecular field analysis (CoMFA) and comparative
molecular similarity indices (CoMSIA). The best CoMFA model was derived from the atom fit alignment with a cross-validated
r
2 (q
2) value of 0.516 and conventional r
2 of 0.950, while the best CoMSIA model yielded a q
2 of 0.455 and r
2 of 0.979 (39 molecules in training set, 9 molecules in test set). The CoMFA and CoMSIA contour maps generated from these
models provided inklings about the influence of interactive molecular fields in the space on the activity. GOLD, Sybyl (FlexX)
and AutoDock docking protocols were exercised to explore the protein–inhibitor interactions. The integration of 3D-QSAR and
molecular docking has proffered essential structural features of pyrazolyl urea inhibitors and also strategies to design new
potent analogues with enhanced activity. 相似文献
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Poulose AC Veeranarayanan S Mohamed MS Raveendran S Nagaoka Y Yoshida Y Maekawa T Kumar DS 《Journal of fluorescence》2012,22(3):931-944
Cancer stands as a leading cause of mortality worldwide and diagnostics of cancer still faces drawbacks. Optical imaging of
cancer would allow early diagnosis, evaluation of disease progression and therapy efficiency. To that aim, we have developed
highly biocompatible PEG functionalized cadmium chalcogenide based three differently luminescent quantum dots (QDs) (CdS,
CdSe and CdTe). Folate targeting scheme was utilized for targeting cancer cell line, MCF-7. We demonstrate the biocompatibility,
specificity and efficiency of our nanotool in detection of cancer cells sparing normal cell lines with retained fluorescence
of functionalized QDs as parental counterpart. This is the first time report of utilizing three differently fluorescent QDs
and we have detailed about the internalization of these materials and time dependent saturation of targeting schemes. We present
here the success of utilizing our biocompatible imaging tool for early diagnosis of cancer. 相似文献
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