Glycosylation site analysis of human alpha-1-acid glycoprotein (AGP) by capillary liquid chromatography-electrospray mass spectrometry

A new anionic surfactant (RapiGest SF) was successfully used for site-specific analysis of glycosylation in human alpha-1-acid glycoprotein (AGP). By means of this analytical approach combined with capillary HPLC-mass spectrometry (and tandem mass spectrometry), the N-linked glycosylation pattern of AGP was explored. On the basis of mass matching and MS/MS experiments ca 80 different AGP-derived glycopeptides were identified. Glycosylation shows a markedly different pattern for the various glycosylation sites. At sites I and II, triantennary complex-type oligosaccharides predominate and at sites III, IV and V, tetra-antennary complex-type oligosaccharides predominate. Sites IV and V show the presence of additional N-acetyl lactosamine (Gal-GlcNAc) units (even higher degree of branching and/or longer antennae are also present).     

CCR5 antagonists: 3-(pyrrolidin-1-yl)propionic acid analogues with potent anti-HIV activity

[reaction: see text] A novel approach to alpha,alpha-disubstituted-beta-amino acids (beta(2,2)-amino acids) was employed in the synthesis of a series of 3-(pyrrolidin-1-yl)propionic acids possessing high affinity for the CCR5 receptor and potent anti-HIV activity. The rat pharmacokinetics for these new analogues featured higher bioavailabilities and lower rates of clearance as compared to cyclopentane 1.     

Quasi-linear Schrödinger–Poisson system under an exponential critical nonlinearity: existence and asymptotic behaviour of solutions

We prove the generalized $$L^p$$ -Poincaré inequalities and Sobolev type inequalities on graphs with polynomial volume growth. They are optimal on Vicsek graphs.     

Enveloping algebras that are principal ideal rings

Let L be a restricted Lie algebra over a field of positive characteristic. We prove that the restricted enveloping algebra of L is a principal ideal ring if and only if L is an extension of a finite-dimensional torus by a cyclic restricted Lie algebra.     

STUDIES OF ADHESION OF METAL PARTICLES TO SILICA PARTICLES BASED ON ZETA POTENTIAL MEASUREMENTS

The zeta-potentials of silica, copper, platinum and gold particles have been measured as a function of pH. The isoelectric points were found to be at pH 3.0, 5.8, 3.0 and 3.5, respectively. In the pH range 3.0 to 5.8 copper and silica particles are oppositely charged and accordingly the coating of silica with copper particles could be demonstrated. In the case of gold and platinum the sign of the charge is such that direct adhesion to silica particles cannot be expected and this was also demonstrated in the case of platinum.     

Synthesis and anticancer properties of gold(I) and silver(I) N-heterocyclic carbene complexes

Bis(1,3-dimethylimidazol-2-ylidene)silver(I) nitrate and bis(4,5-dichloro-1,3-dimethylimidazol-2-ylidene)silver(I) nitrate were prepared by reacting the corresponding imidazolium nitrate salts with silver oxide. Bis(1,3-dimethylimidazol-2-ylidene)gold(I) nitrate and bis(4,5-dichloro-1,3-dimethylimidazol-2-ylidene)gold(I) nitrate salts were prepared via transmetallation of their silver precursors with chloro dimethylsulfide gold. The anticancer properties were determined using NCI-H460 lung cancer cells. Efficacy was established by comparison of the gold and silver compounds with cisplatin.     

An archaeometric approach about the study of medieval glass from Siponto (Foggia, Italy)

Medieval glasses, including feet and rims of chalices, fragments of lamps and globular bottles, coming from the archaeological site of Siponto (Foggia, Italy), were analyzed by Inductively Coupled Plasma Emission Spectroscopy and Graphite Furnace Atomic Absorption Spectroscopy for investigating and defining glass production technology in Apulia (Italy) in the Middle Ages, because of the poor understanding currently achieved on either compositional and technological features of medieval glass items. The examined finds, whether colourless or coloured blue, yellow-green, yellow, pink and red, revealed a typical silica-soda-lime-composition. The chemical analysis and the statistical treatment of data allowed to trace former, flux, modifier and, where it is present, the element responsible for colour, clarifying production technology issues. It has been possible to identify, moreover, objects obtained by recycling of cullets or finished items.Finally, this work evaluates the effectiveness of the statistical multivariate treatment by Principal Component Analysis (PCA), Clustering Analysis (CA) and Factor Analysis (FA) on compositional data to obtain technological information in opposition to the conventional binary oxides diagram, which represent the most common, widely assessed, archaeometrical practice to obtain technological information from compositional data.