排序方式: 共有5条查询结果,搜索用时 15 毫秒
1
1.
Applied Biochemistry and Biotechnology - A novel, anaerobic fermentation process is developed to produce calcium magnesium acetate (CMA) from cheese whey. CMA can be used as a noncorrosive road... 相似文献
2.
Bai Y Koh CG Boreman M Juang YJ Tang IC Lee LJ Yang ST 《Langmuir : the ACS journal of surfaces and colloids》2006,22(22):9458-9467
A novel surface treatment method using poly(ethyleneimine) (PEI), an amine-bearing polymer, was developed to enhance antibody binding on the poly(methyl methacrylate) (PMMA) microfluidic immunoassay device. By treating the PMMA surface of the microchannel on the microfluidic device with PEI, 10 times more active antibodies can be bound to the microchannel surface as compared to those without treatment or treated with the small amine-bearing molecule, hexamethylenediamine (HMD). Consequently, PEI surface modification greatly improved the immunoassay performance of the microfluidic device, making it more sensitive and reliable in the detection of IgG. The improvement can be attributed to the spacer effect as well as the functional amine groups provided by the polymeric PEI molecules. Due to the smaller dimensions (140x125 microm) of the microchannel, the time required for antibody diffusion and adsorption onto the microchannel surface was reduced to only several minutes, which was 10 times faster than the similar process carried out in 96-well plates. The microchip also had a wider detection dynamic range, from 5 to 1000 ng/mL, as compared to that of the microtiter plate (from 2 to 100 ng/mL). With the PEI surface modification, PMMA-based microchips can be effectively used for enzyme linked immunosorbent assays (ELISA) with a similar detection limit, but much less reagent consumption and shorter assay time as compared to the conventional 96-well plate. 相似文献
3.
Asmaa Abdella Ashraf F. El-Baz Ibrahim A. Ibrahim Emad Eldin Mahrous Shang-Tian Yang 《Natural product research》2018,32(20):2382-2391
β-glucosidase enzyme produced from Aspergillus niger NRRL 3122 has been partially purified and characterised. Its molecular weight was 180 KDa. The optimal pH and temperature were 3.98 and 55 °C, respectively. It promoted the hydrolysis of soy flour isoflavone glycosides to their aglycone. Two-level Plackett–Burman design was applied and effective variables for genistein production were determined. Reaction time had a significant positive effect, and pH had a significant negative effect. They were further evaluated using Box–Behnken model. Accordingly, the optimal combination of the major reaction affecting factors was reaction time, 5 h and pH, 4. The concentration of genistein increased by 11.73 folds using this optimal combination. The antioxidant activity of the non-biotransformed and biotransformed soy flour extracts was determined by DPPH method. It was found that biotransformation increased the antioxidant activity by four folds. 相似文献
4.
Hanjing Huang Shang-Tian Yang David E. Ramey 《Applied biochemistry and biotechnology》2004,114(1-3):671-688
An energy-efficient hollow-fiber membrane extraction process was successfully developed to separate and recover lactic acid
produced in fermentation. Although many fermentation processes have been developed for lactic acid production, and economical
method for lactic acid recovery from the fermentation broth is still needed. Continuous extraction of lactic acid from a simulated
aqueous stream was achieved by using Alamine 336 in 2-octanol contained in a hollow-fiber membrane extractor. In this process,
the extractant was simultaneously regenerated by stripping with NaOH in a second membrane extractor, and the final product
is a concentrated lactate salt solution. The extraction rate increased linearly with an increase in the Alamine 336 content
in the solvent (from 5 to 40%). Increasing the concentration of the undissociated lactic acid in the feed solution by either
increasing the lactate concentration (from 5 to 40 g/L) or decreasing the solution pH (from 5.0 to 4.0) also increased the
extraction rate. Based on these observations, a reactive extraction model with a first-order reaction mechanism for both lactic
acid and amine concentrations was proposed. The extraction rate also increased with an increase in the feed flow rate, but
not the flow rates of solvent and the stripping solution, suggesting that the process was not limited by diffusion in the
liquid films or membrane pores. A mathematical model considering both diffusion and chemical reaction in the extractor and
back extractor was developed to simulate the process. The model fits the experimental data well and can be used in scale up
design of the process. 相似文献
5.
Wei-Cho Huang David E. Ramey Shang-Tian Yang 《Applied biochemistry and biotechnology》2004,115(1-3):887-898
We explored the influence of dilution rate and pH in continuous cultures of Clostridium acetobutylicum. A 200-mL fibrous bed bioreactor was used to produce high cell density and butyrate concentrations at pH 5.4 and 35°C. By
feeding glucose and butyrate as a cosubstrate, the fermentation was maintained in the solventogenesis phase, and the optimal
butanol productivity of 4.6g/(L h) and a yield of 0.42 g/g were obtained at a dilution rate of 0.9h−1 and pH 4.3. Compared to the conventional acetone-butanol-ethanol fermentation, the new fermentation process greatly improved
butanol yield, making butanol production from corn an attractive alternative to ethanol fermentation. 相似文献
1