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Wang J Even MA Chen X Schmaier AH Waite JH Chen Z 《Journal of the American Chemical Society》2003,125(33):9914-9915
In this Communication, we demonstrate the novel observation that it is feasible to collect amide signals from polymer/protein solution interfaces in situ using sum frequency generation (SFG) vibrational spectroscopy. Such SFG amide signals allow for acquisition of more detailed molecular level information of entire interfacial protein structures. Proteins investigated include bovine serum albumin, mussel protein mefp-2, factor XIIa, and ubiquitin. Our studies indicate that different proteins generate different SFG amide signals at the polystyrene/protein solution interface, showing that they have different interfacial coverage, secondary structure, or orientation. 相似文献
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Chen X Wang J Paszti Z Wang F Schrauben JN Tarabara VV Schmaier AH Chen Z 《Analytical and bioanalytical chemistry》2007,388(1):65-72
Electrostatic interactions between negatively charged polymer surfaces and factor XII (FXII), a blood coagulation factor,
were investigated by sum frequency generation (SFG) vibrational spectroscopy, supplemented by several analytical techniques
including attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), quartz crystal microbalance (QCM),
ζ-potential measurement, and chromogenic assay. A series of sulfonated polystyrenes (sPS) with different sulfonation levels
were synthesized as model surfaces with different surface charge densities. SFG spectra collected from FXII adsorbed onto
PS and sPS surfaces with different surface charge densities showed remarkable differences in spectral features and especially
in spectral intensity. Chromogenic assay experiments showed that highly charged sPS surfaces induced FXII autoactivation.
ATR-FTIR and QCM results indicated that adsorption amounts on the PS and sPS surfaces were similar even though the surface
charge densities were different. No significant conformational change was observed from FXII adsorbed onto surfaces studied.
Using theoretical calculations, the possible contribution from the third-order nonlinear optical effect induced by the surface
electric field was evaluated, and it was found to be unable to yield the SFG signal enhancement observed. Therefore it was
concluded that the adsorbed FXII orientation and ordering were the main reasons for the remarkable SFG amide I signal increase
on sPS surfaces. These investigations indicate that negatively charged surfaces facilitate or induce FXII autoactivation on
the molecular level by imposing specific orientation and ordering on the adsorbed protein molecules.
Electronic supplementary material Supplementary material is available in the online version of this article at and is accessible for authorized users. 相似文献
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In this article, we derive the intrinsic equations for a generalized relaxed elastic line on an oriented surface in the Galilean 3-dimensional space G3. These equations will give direct and more geometric approach to questions concerning about generalized relaxed elastic lines on an oriented surface in G3. 相似文献
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Lenneke WA van Hooijdonk Muhammad Ichwan Thomas F Dijkmans Theo G Schouten Marijke WA de Backer Roger AH Adan Fons J Verbeek Erno Vreugdenhil Carlos P Fitzsimons 《BMC neuroscience》2009,10(1):2-19
Background
In the adult hippocampus, the granule cell layer of the dentate gyrus is a heterogeneous structure formed by neurons of different ages, morphologies and electrophysiological properties. Retroviral vectors have been extensively used to transduce cells of the granule cell layer and study their inherent properties in an intact brain environment. In addition, lentivirus-based vectors have been used to deliver transgenes to replicative and non-replicative cells as well, such as post mitotic neurons of the CNS. However, only few studies have been dedicated to address the applicability of these widespread used vectors to hippocampal cells in vivo. Therefore, the aim of this study was to extensively characterize the cell types that are effectively transduced in vivo by VSVg-pseudotyped lentivirus-based vectors in the hippocampus dentate gyrus. 相似文献9.
Marijke WA de Backer Carlos P Fitzsimons Maike AD Brans Mieneke CM Luijendijk Keith M Garner Erno Vreugdenhil Roger AH Adan 《BMC neuroscience》2010,11(1):81
Background
This study compared the transduction efficiencies of an adeno-associated viral (AAV) vector, which was pseudotyped with an AAV1 capsid and encoded the green fluorescent protein (GFP), with a lentiviral (LV) vector, which was pseudotyped with a VSV-G envelop and encoded the discosoma red fluorescent protein (dsRed), to investigate which viral vector transduced the lateral hypothalamus or the amygdala more efficiently. The LV-dsRed and AAV1-GFP vector were mixed and injected into the lateral hypothalamus or into the amygdala of adult rats. The titers that were injected were 1 × 108 or 1 × 109 genomic copies of AAV1-GFP and 1 × 105 transducing units of LV-dsRed. 相似文献10.
The equations of magnetostatic equilibria for a plasma in agravitational field are investigated analytically. For equilibriawith an ignorable spatial coordinate, the equations reduce toa single nonlinear elliptic equation for the magnetic potentialu known as the Grad-Shafranov equation. By specifying the arbitraryfunctions in this equation, a Liouville equation is obtained.Bäcklund transformations are described and applied to obtainexact solutions for the Liouville equation modelling an isothermalmagnetostatic atmosphere, in which the current density J isproportional to the exponential of the magnetic potential andmoveover falls off exponentially with distance vertical to thebase with an e-folding distance equal to the gravitational scaleheight. 相似文献