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Gnanasekar Rani Sarnaik Jayula S. Joseph N. C. Kadwad V. B. Mathur Anupam 《Journal of Radioanalytical and Nuclear Chemistry》2021,329(1):71-76
Journal of Radioanalytical and Nuclear Chemistry - We describe a simple, user friendly two-step radioimmunoassay (RIA) based on antibody coated tubes for the measurement of free triiodothyronine in... 相似文献
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R. R. Rasmi K. Bhasker Shenoy Jayula Sarnaik V. B. Kadwad H. M. Somashekarappa N. Sivaprasad 《Journal of Radioanalytical and Nuclear Chemistry》2014,302(3):1271-1275
We describe a convenient and flexible solid phase radioimmunoassay for human insulin employing magnetizable cellulose particles. Anti-porcine insulin antibody was covalently linked to magnetizable cellulose particles to form a stable and economical solid phase immunosorbent system. The tracer was prepared by radioiodinating insulin with 125I using Chloramine-T oxidation method. The analytical sensitivity of assay observed was 5.5 μIU/mL. Intra-assay and inter-assay variations were found to be <12 % along with analytical recovery of 93–109 %. The developed assay can be used for the routine analysis of clinical samples. In addition, concentration of the solid phase magnetizable immunosorbent can be easily varied as per the specific requirement for research purposes. 相似文献
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Lay-Kien YangRobert P. Glover K. YoganathanJayant P. Sarnaik Archana J. GodboleDoel D. Soejarto Antony D. BussMark S. Butler 《Tetrahedron letters》2003,44(31):5827-5829
A novel naphthylisoquinoline alkaloid, ancisheynine 1, was isolated from the aerial part of Ancistrocladus heyneanus Wallich ex J. Graham (Ancistrocladaceae). Ancisheynine 1 contained a previously undescribed N-2,C-8′-linkage between the isoquinoline and naphthalene units and its structure was established by spectroscopic methods. 相似文献
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