Effects of Roxadustat on Erythropoietin Production in the Rat Body

Anemia is a major complication of chronic renal failure. To treat this anemia, prolylhydroxylase domain enzyme (PHD) inhibitors as well as erythropoiesis-stimulating agents (ESAs) have been used. Although PHD inhibitors rapidly stimulate erythropoietin (Epo) production, the precise sites of Epo production following the administration of these drugs have not been identified. We developed a novel method for the detection of the Epo protein that employs deglycosylation-coupled Western blotting. With protein deglycosylation, tissue Epo contents can be quantified over an extremely wide range. Using this method, we examined the effects of the PHD inhibitor, Roxadustat (ROX), and severe hypoxia on Epo production in various tissues in rats. We observed that ROX increased Epo mRNA expression in both the kidneys and liver. However, Epo protein was detected in the kidneys but not in the liver. Epo protein was also detected in the salivary glands, spleen, epididymis and ovaries. However, both PHD inhibitors (ROX) and severe hypoxia increased the Epo protein abundance only in the kidneys. These data show that, while Epo is produced in many tissues, PHD inhibitors as well as severe hypoxia regulate Epo production only in the kidneys.     

The investigation of an equilibrium dependent reaction for the formation of enamines in a microchemical system

The paper describes the equilibrium dependant reaction for the formation of enamines in a microchemical system utilising electroosmotic flow (EOF) for fluid mobilisation. The authors have shown that the reaction can be carried out without the presence of a Lewis acid catalyst, in addition the enamine intermediate was synthesised at room temperature using mild solvent conditions. A 42% conversion of cyclohexanone into the enamine has been achieved to date.     

A size-width inequality for distributive lattices

We show that every collection ofw sets such that none contains any other generates at least 3w-2 sets under the operations of taking intersections and unions. In particular, we prove that if the finite distributive lattice ? contains an antichain of sizew, then |?| ≧3w, forw≠1, 2, 3, 6, where the minimal exceptional cases arise from the Boolean algebras ?_n withn=0, 1, 2, 3, 4 atoms.     

Simplified monoharmonic approach to investigation of forced vibrations of thin wall multilayer inelastic elements with piezoactive layers under cyclic loading

A coupled dynamic problem of electromechanics for thin wall multilayer elements is formulated based on the Kirchhoff–Love hypotheses. In the case of harmonic loading, a simplified formulation is given using the monoharmonic approach and the concept of complex moduli to characterize the cyclic properties of the material. The problem of forced vibrations of three-layer beam, whose outer layers are made of a viscoelastic piezoactive material, and, the inner layer of a passive physically nonlinear material, is considered as an example to demonstrate the possibility of the technique elaborated. The possibility of damping the forced vibrations of a structure with the help of harmonic voltages applied to the external piezoactive layers is studied. Results obtained for the transient response of the beam using the complete model are compared with data found using the simplified model. Limitations on the simplified model application are specified.     

On a problem involving bounded epimorphisms of lattices

A latticeL satisfies thebounded epimorphism condition if wheneverM is a lattce and ϕ:M →L is a bounded epimorphism, there exists a homomorphismι:L →M such that ιϕ=id _L . we show that the class of finite lattices satisfying the bounded epimorphism condition is properly contained in the class of finite lattices satisfying Whitman's condition (W). We also introduce a property defined for finite lattices that is sufficient to imply the bounded epimorphism condition. Presented by B. Jónsson.