Influence of Food Restriction and Compensatory Growth on Protein Synthesis and Breakdown in Growing Rats

The experiment was carried out with 24 male albino rats (5 weeks old, live weight 79 ± 5.1 g) divided into 2 groups. Gr. 1 (control group) was fed the commercial breeding food Ssniff with 22.4% crude protein ad lib., Gr. 2 (test group) for the first fortnight a mixture of the breeding food/cellulose 60/40 (restriction period) and subsequently for the second fortnight (compensatory period) also the breeding food ad lib. During both of these periods an 8 day N balance trial and a 4 day ¹⁵N tracer trial were included for estimation of growth rate and protein synthesis rate. Protein synthesis was ascertained by the endproduct method (oral application, single dose, mixture of 12 ¹⁵N-labelled amino acids); protein breakdown was calculated as the difference of protein synthesis rate and growth rate. The estimated rates were converted into fractional ones by referring to the body N content of corresponding animals. From the results of this experiment is concluded: The smaller N balance during moderate nutritional restriction can be attributed to a decrease of protein synthesis. The compensatory growth of the animals during the realimentation period is caused by an increase of protein synthesis and the enhanced protein conversion. Protein degradation is nearly unaffected.     

The Art of Titration. From Classical End Points to Modern Differential and Dynamic Analysis

Titration represents the quantitative determination of a chemical change in response to the variation in concentration of a standrad. Information on reaction parameters is usually obtained from an analysis of the shape of the titration curve. In this paper the derivatives of titration functions with respect to concentration variables and with respect to equilibrium parameters are compared with one another. The latter can be determined directly from amplitudes and time constants of the dynamic responses to equilibrium perturbations, brought about by fast alterations of temperature, pressure or electirc field. Three general situations have to be distinguished: 1. If the interaction is very strong (i.e. the complex stability is high: K → ∞), the (average) number of sample praticles can be counted directly by means of their reaction with a calibrated number of standard particles.—2. If, on the contrary, the interaction is weak (i.e. the complex stability is low), the sample will react quantitatively only in the presence of a large excess of standard; the half-way point of which provides a direct measure of the binding constant.—3. Only for moderate interactions can individual curves characteristic of the binding strength be observed. The limiting cases 1 and 2 yield titration curves which reflect only the general class to which the system belongs. The dynamical procedures are fairly insensitive in case 1, which represents the classical end-point situation and is most suitable for quantitative anlaysis, i.e. for a determination of sample concentrations. For case 2, the classical and dynamical techniques yield comparable information: mass-action parameters are obtained from the extrema of the curves, which occur only in the presence of an excess of the standard. In case 3 the dynamical treatment provides a more direct access to the equilibrium constants, and also to the enthalpies or volumes of reaction as well as to the rate constants. The advantage of the dynamic method is due to the fact that in the two derivatives of the titration function d T_i/d ln q and d T_i/d ln p (where q is the titration variable, i.e. the ratio of standard and sample concentration, and p the mass-action parameter, i.e. a reduced binding constant) the terms resulting from a differentiation with respect to p are more closely related to the reaction parameters than those following from a variation of q. The dynamic analysis is generalized for applications to multiple-step titration processes, where it allows for the measurement fo equilibrium and rate parameters of individual steps. A uniform representation utilizing trigonometric functions was chosen which expresses clearly the singualr character of the end-point. The relations are summarized intabular form. They provide in conjunction with the illustrations the basis for a comparative discussion.