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1.
The pyrophoric compound Rb2[U(NH2)6] was obtained as a grey to black powder from the reaction of more than three equivalents of RbNH2 with UI3 in anhydrous liquid ammonia. During the process, UIII is oxidized to UIV and ammonia is reduced under evolution of H2. Rb2[U(NH2)6] crystallizes in the cubic crystal system, space group Fm3 m, with the lattice parameter a = 9.7870(12) Å, V = 937.4(2) Å3, Z = 4 at T = 293 K. It is isotypic to K2PtCl6. The compound contains the unprecedented hexaamidouranate(IV) anion [U(NH2)6]2–. 相似文献
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A discussion is given of the relation between the maximum functionaltheorem and Schwinger's variational principle, and it is shownthat the bound given by Schwinger's principle is better thanor the same as that given by the maximum functional theorem. 相似文献
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Van Zandt LL 《Physical review letters》1986,57(16):2085-2087
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About 2000 protein spots of human Jurkat T-cells were detected by high resolution two-dimensional gel electrophoresis (2-DE) and were characterized in terms of their isoelectric point and molecular mass. A 2-DE database was constructed and is available at http://www.mpiib-berlin.mpg.de/2D-PAGE/. At present the database contains 67 identified protein spots. These proteins were identified after tryptic digestion by peptide mass fingerprinting with delayed extraction-matrix assisted laser desorption/ionization-mass spectrometry (DE-MALDI-MS). Proteins with a sequence coverage of at least 30% were introduced in the database. This sequence coverage could not always be obtained by using only the matrix alpha-cyano-4-hydroxycinnamic acid (CHCA) for the mass analysis. Therefore, an additional mass spectrum was recorded by using 2,5-dihydroxybenzoic acid (DHB). Usually, additional mass peaks were detected and together with the mass spectrum of CHCA this resulted in the desired sequence coverage. 相似文献
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Following the variational principles for linear initial valueproblems associated with the wave and heat conduction equationsdiscussed by Gurtin and Leitmann and using time convolutionsit is shown that general variational principles exist for theseproblems with sources on the boundaries and within the regionunder consideration. 相似文献
9.
Modified Locally One Dimensional Methods for Parabolic Partial Differential Equations in Two Space Variables 总被引:1,自引:0,他引:1
In this note we consider the locally one dimensional methodas discussed in Gourlay & Mitchell (1969, 1972) and indicatehow this scheme can be used to solve accurately a parabolicpartial differential equation in two space variables. 相似文献
10.
Quantitative proteome analysis of cisplatin-induced apoptosis in total Jurkat T cell lysates was performed in order to identify modified proteins. Proteins were labeled in cell culture with stable isotopes of arginines, and fractionated by SDS-PAGE. Subsequently, tryptic peptides were analyzed by nano-LC coupled offline to MALDI-TOF/TOF-MS as an alternative to commonly used online LC-ESI-MS. As a result, 26 proteins were found with a relative abundance higher than 1.5, thereof 19 already known and seven unknown to be involved in apoptosis (adenine phosphoribosyltransferase, microsomal signal peptidase 25 kDa subunit, phosphomevalonate kinase, probable rRNA processing protein EBP2, RNA-binding protein 4, transmembrane protein 33, and tetratricopeptide repeat domain 9C). Immunoblotting of core-binding factor beta and elongation factor 2 revealed similar quantitative changes as detected by the SILAC-based proteomics approach. Strikingly, 8 of 26 identified apoptosis-modified proteins contained at least one RNA-binding motif. Three caspase cleavage sites of the 54 kDa nuclear RNA-binding protein (p54nrb) were mapped at DQLD(231) (downward arrow)D, DQVD(286) (downward arrow)R, and MMPD(422) (downward arrow)G by applying caspase-3 to the in vitro translated protein and mutation analysis. The determined caspase cleavage sites were located C-terminal to the two RNA-binding motifs and one (DQLD(231) (downward arrow)D) within the NOPS domain of p54nrb. Concisely, quantitative protein data generated by offline LC-MALDI-MS were shown to be particularly accurate. Furthermore, only regulated peptides were selected in a result-dependent manner for MS/MS analyses and revealed novel apoptosis-modified proteins. 相似文献