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1.
Scott B. Ficarro Jessica M. Biagi Jinhua Wang Jenna Scotcher Rositsa I. Koleva Joseph D. Card Guillaume Adelmant Huan He Manor Askenazi Alan G. Marshall Nicolas L. Young Nathanael S. Gray Jarrod A. Marto 《Journal of the American Society for Mass Spectrometry》2014,25(4):636-650
We assemble a versatile molecular scaffold from simple building blocks to create binary and multiplexed stable isotope reagents for quantitative mass spectrometry. Termed Protected Amine Labels (PAL), these reagents offer multiple analytical figures of merit including, (1) robust targeting of peptide N-termini and lysyl side chains, (2) optimal mass spectrometry ionization efficiency through regeneration of primary amines on labeled peptides, (3) an amino acid-based mass tag that incorporates heavy isotopes of carbon, nitrogen, and oxygen to ensure matched physicochemical and MS/MS fragmentation behavior among labeled peptides, and (4) a molecularly efficient architecture, in which the majority of hetero-atom centers can be used to synthesize a variety of nominal mass and sub-Da isotopologue stable isotope reagents. We demonstrate the performance of these reagents in well-established strategies whereby up to four channels of peptide isotopomers, each separated by 4 Da, are quantified in MS-level scans with accuracies comparable to current commercial reagents. In addition, we utilize the PAL scaffold to create isotopologue reagents in which labeled peptide analogs differ in mass based on the binding energy in carbon and nitrogen nuclei, thereby allowing quantification based on MS or MS/MS spectra. We demonstrate accurate quantification for reagents that support 6-plex labeling and propose extension of this scheme to 9-channels based on a similar PAL scaffold. Finally, we provide exemplar data that extend the application of isotopologe-based quantification reagents to medium resolution, quadrupole time-of-flight mass spectrometers. Figure
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2.
Mihail Mihaylov Elena Ivanova Videlina Zdravkova Stanislava Andonova Nikola Drenchev Kristina Chakarova Radoslav Kefirov Rositsa Kukeva Radostina Stoyanova Konstantin Hadjiivanov 《Molecules (Basel, Switzerland)》2022,27(1)
The transition to a hydrogen economy requires the development of cost-effective methods for purifying hydrogen from CO. In this study, we explore the possibilities of Cu/ZSM-5 as an adsorbent for this purpose. Samples obtained by cation exchange from aqueous solution (AE) and solid-state exchange with CuCl (SE) were characterized by in situ EPR and FTIR, H2-TPR, CO-TPD, etc. The AE samples possess mainly isolated Cu2+ cations not adsorbing CO. Reduction generates Cu+ sites demonstrating different affinity to CO, with the strongest centres desorbing CO at about 350 °C. The SE samples have about twice higher Cu/Al ratios, as one H+ is exchanged with one Cu+ cation. Although some of the introduced Cu+ sites are oxidized to Cu2+ upon contact with air, they easily recover their original oxidation state after thermal treatment in vacuum or under inert gas stream. In addition, these Cu+ centres regenerate at relatively low temperatures. It is important that water does not block the CO adsorption sites because of the formation of Cu+(CO)(H2O)x complexes. Dynamic adsorption studies show that Cu/ZSM-5 selectively adsorbs CO in the presence of hydrogen. The results indicate that the SE samples are very perspective materials for purification of H2 from CO. 相似文献
3.
Ivo Grabchev Evgenia Vasileva-Tonkova Desislava Staneva Paula Bosch Rositsa Kukeva Radostina Stoyanova 《International Journal of Polymer Analysis and Characterization》2018,23(1):45-57
A new polyamidoamine metallodendrimer modified with eight 1,8-naphthalimide units was synthesized. The Cu(II) complex has been investigated by EPR spectroscopy and it has shown that 17 copper ions have involved in the dendrimer complex. To confirm the presence of metallodendrimers on the cotton surface, scanning electron microscopy characterization has been used. In vitro antimicrobial activity of the metallodendrimer against different pathogens was investigated and compared to the dendrimer ligand free of copper ions. Both dendrimers were deposited on a cotton fabric and antibacterial activity of the treated cotton samples was investigated against model Gram-positive and Gram-negative bacteria. It has been shown that the studied dendrimers reduce bacterial growth and prevent the formation of biofilms. The metallodendrimer showed stronger antimicrobial and biofilm inhibiting abilities than those of the free of Cu(II) ions ligand. 相似文献
4.
Implementation of a mixed-mode solid-phase extraction is discussed as a promising approach for matrix clean-up in multiresidue/multimatrix methods. Sorbents characterized by different mechanisms of sorption such as reversed-phase (graphitized carbon), weak anion exchange (primary-secondary amine) and strong anion exchange (quaternary amine) were studied for their effectiveness in the removal of the matrix co-extractives in grains, fruits and vegetables for trace pesticide residues analysis. The pesticide residues were determined by gas chromatography with electron capture and mass spectrometric detection. Recoveries data of 25 pesticides from different groups at 0.01 mg/kg level ranged between 73 and 117% and with standard deviation below 15%. The limits of quantification were below or at 0.01 mg/kg. 相似文献
5.
Georgy Grancharov Mariya-Desislava Atanasova Radostina Kalinova Rositsa Gergova Georgi Popkirov Christosko Dikov Marushka Sendova-Vassileva 《Molecules (Basel, Switzerland)》2021,26(22)
In this study, some crucial parameters were determined of flexible polymer–organic solar cells prepared from an active layer blend of poly(3-hexylthiophene) (P3HT) and the fullerene derivative [6,6]-phenyl-C61-butyric acid methyl ester (PCBM) mixed in 1:1 mass ratio and deposited from chlorobenzene solution by spin-coating on poly(ethylene terephthalate) (PET)/ITO substrates. Additionally, the positive effect of an electron transport layer (ETL) prepared from zinc oxide nanoparticles (ZnO np) on flexible photovoltaic elements’ performance and stability was investigated. Test devices with above normal architecture and silver back electrodes deposed by magnetron sputtering were constructed under environmental conditions. They were characterized by current-voltage (I–V) measurements, quantum efficiency, impedance spectroscopy, surface morphology, and time–degradation experiments. The control over morphology of active layer thin film was achieved by post-deposition thermal treatment at temperatures of 110–120 °C, which led to optimization of device morphology and electrical parameters. The impedance spectroscopy results of flexible photovoltaic elements were fitted using two R||CPE circuits in series. Polymer–organic solar cells prepared on plastic substrates showed comparable current–voltage characteristics and structural properties but need further device stability improvement according to traditionally constructed cells on glass substrates. 相似文献
6.
Tony Venelinov Veronika Mihaylova Rositsa Peycheva Miroslav Todorov Galina Yotova Boyan Todorov Valentina Lyubomirova Stefan Tsakovski 《Molecules (Basel, Switzerland)》2021,26(24)
The temporal dynamics of anthropogenic impacts on the Pchelina Reservoir is assessed based on chemical element analysis of three sediment cores at a depth of about 100–130 cm below the surface water. The 137Cs activity is measured to identify the layers corresponding to the 1986 Chernobyl accident. The obtained dating of sediment cores gives an average sedimentation rate of 0.44 cm/year in the Pchelina Reservoir. The elements’ depth profiles (Ti, Mn, Fe, Zn, Cr, Ni, Cu, Mo, Sn, Sb, Pb, Co, Cd, Ce, Tl, Bi, Gd, La, Th and Unat) outline the Struma River as the main anthropogenic source for Pchelina Reservoir sediments. The principal component analysis reveals two groups of chemical elements connected with the anthropogenic impacts. The first group of chemical elements (Mn, Fe, Cr, Ni, Cu, Mo, Sn, Sb and Co) has increasing time trends in the Struma sediment core and no trend or decreasing ones at the Pchelina sampling core. The behavior of these elements is determined by the change of the profile of the industry in the Pernik town during the 1990s. The second group of elements (Zn, Pb, Cd, Bi and Unat) has increasing time trends in Struma and Pchelina sediment cores. The increased concentrations of these elements during the whole investigated period have led to moderate enrichments for Pb and Unat, and significant enrichments for Zn and Cd at the Pchelina sampling site. The moderately contaminated, according to the geoaccumulation indexes, Pchelina Reservoir surface sediment samples have low ecotoxicity. 相似文献
7.
Bogdan Goranov Desislava Teneva Rositsa Denkova-Kostova Vesela Shopska Nadia Oulahal Zapryana Denkova Georgi Kostov Pascal Degraeve Rafael Pagan 《Molecules (Basel, Switzerland)》2022,27(17)
Probiotic bacteria are used for food biopreservation because their metabolic products might contribute to ensuring food microbiological safety and/or increase its shelf life without the addition of chemical preservatives. Moreover, biopreserved foods are excellent vehicles for the delivery of probiotic bacteria. The aim of the study was to investigate the potential of chocolate mousse food matrix for the delivery of the probiotic strain Lactobacillus helveticus 2/20 (Lb. helveticus 2/20) and to investigate its capacity to inhibit the growth of two foodborne pathogenic bacteria (Staphylococcus aureus and Escherichia coli). Therefore, the populations of free or encapsulated in calcium alginate Lb. helveticus 2/20 cells and/or of each pathogen (used to voluntarily contaminate each sample) were monitored both in complex nutrient medium (MRS broth) and in chocolate mousse under refrigeration conditions and at room temperature. Lb. helveticus 2/20 alone in free or encapsulated state effectively inhibited the growth of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 in chocolate mousse when stored at 20 ± 2 °C. Practically no viable unwanted bacteria were identified on the 7th day from the beginning of the process. High viable Lb. helveticus 2/20 cell populations were maintained during storage under refrigerated conditions (4 ± 2 °C) and at room temperature. Chocolate mousse is thus a promising food matrix to deliver probiotic Lb. helveticus 2/20 cells, which could also protect it from contamination by unwanted bacteria. 相似文献
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9.
Prof. Dr. Liudmil Antonov Dr. Vera Deneva Dr. Svilen Simeonov Prof. Dr. Vanya Kurteva Dr. Aurelien Crochet Prof. Dr. Katharina M. Fromm Dr. Boris Shivachev Prof. Dr. Rositsa Nikolova Dr. Marika Savarese Prof. Dr. Carlo Adamo 《Chemphyschem》2015,16(3):649-657
A series of new tautomeric azonaphthols are synthesized and the possibilities for molecular switching are investigated using molecular spectroscopy, X‐ray analysis and density functional theory quantum chemical calculations. Two opposite effects that influence switching are studied: attaching a piperidine sidearm, and adding substituents to the phenyl ring. On the one hand, the attached piperidine moiety stabilizes the enol form leading to a controlled shift of the equilibrium upon protonation. On the other hand, the relative stability of the azonaphthol tautomers strongly depends on the effects of the substituents on the phenyl ring: electron donors tend to stabilize the enol tautomer, whereas electron acceptors lead to stabilization of the keto form. However, these effects do not shift fully the equilibrium towards either of the tautomers. Nevertheless, the effect of the substituents can be an additional tool to affect the switching between “on” and “off” states. Electron‐withdrawing substituents stabilize the keto form and impede switching to the off state, whereas electron donors stabilize the enol form. The effect of the piperidine unit is dominant overall, and with strongly electron‐withdrawing substituents at the phenyl ring, the enol form exists as a zwitterion. 相似文献
10.
Some drawbacks of the phosphate modifier such as reagent blank contribution and background absorption in electrothermal atomic absorption spectrometric determination of cadmium and lead are substantially alleviated by application of small amounts of phosphate, approximately 0.2 mumol (25 mug of NH(4)H(2)PO(4) or (NH(4))(2)HPO(4)), on the integrated platform of transversely heated graphite atomizer pre-treated with 2.7 mumol of Zr (250 mug) or W (500 mug) and 0.1 mumol of Ir (20 mug). Pyrolysis temperatures for Cd and Pb are up to 900 and 1100 degrees C for aqueous solutions and within 400-600 degrees C (Cd) and 750-850 degrees C (Pb) for biological fluids (urine, blood) and tissues (hair, liver, muscle) solubilized with tetraethylammonium hydroxide. The thermally stabilized phosphate on Zr-Ir or W-Ir treated platforms serves as a permanent modifier in analyses of environmental waters by multiple hot injections of large sample aliquots. Applications to water and biological certified reference materials are tabulated and show good agreement with certified values. Characteristic masses are 0.7-1.0 pg for Cd and 26-31 pg for Pb. 相似文献