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A procedure for the native two-dimensional electrophoresis of peanut and hazelnut proteins is described. Proteins were solubilised after acetone treatment using a combination of 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) and tetramethylene sulphone. These extracts were analysed by a combination of isoelectric focusing in the presence of lactose in immobilized pH gradients followed by charge shift electrophoresis. Immunoblot analysis, using sera from nut allergic patients, allowed the identification of a peanut and hazelnut allergen with identical isoelectric point and apparent molecular mass. These proteins were recovered from duplicate gels using a mixture of formic acid, acetonitrile (ACN) and isopropanol. The molecular masses for both proteins, determined by matrix assisted laser desorption/ionisation-mass spectrometry (MALDI-MS), were 4826 Da. 相似文献
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In this Letter we report observations of optically induced self-organization of colloidal arrays in the presence of unpatterned counterpropagating evanescent waves. The colloidal arrays formed along the laser propagation axis are shown to be linked to the breakup of the incident field into optical spatial solitons, the lateral spacing of the arrays being related to modulation instability of the soft condensed matter system. 相似文献
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We present an approach to realizing enhanced upconversion efficiency in erbium (Er)-doped photonic crystals. Slow-light-mode pumping of the first Er excited state transition can result in enhanced emission from higher-energy levels that may lead to finite subbandgap external quantum efficiency in crystalline silicon solar cells. Using a straightforward electromagnetic model, we calculate potential field enhancements of more than 18× within he slow-light mode of a one-dimensional photonic crystal and discuss design trade-offs and considerations for photovoltaics. 相似文献
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The physical significance of the crack extension force produced by mechanical loads and electric fields in linear dielectric and piezoelectric materials is examined using simple thermodynamic arguments. General expressions are derived for the crack extension force in dielectrics and piezoelectrics, under mechanical and electrical loads, in terms of the measurable parameters elastic compliance and electric capacitance . It is shown that the crack extension force produced by an electric field on an impermeable crack is always negative and it is argued that under combined electromechanical loads the total crack extension force in a piezoelectric cannot be separated into a mechanical component and an electrical component. Expressions for the crack extension force in terms of mechanical and electrical intensity factors are also given. Their derivation from available solutions for the electromechanical field at the crack tip (in a transversely isotropic material of crystal class 6 mm) is presented in detail to emphasize the physical significance of the coefficients that appear in the final expressions. In the light of these results, existing experimental observations that appear to be inconsistent with theoretical expectations are re-examined. The suggestion that the crack extension force is not a valid parameter to characterize the fracture behaviour of ferroelectrics is justified on physical grounds. Its importance is discussed and the rate of mechanical work of fracture is proposed as a more suitable parameter for those cases where the electric field does not produce dielectric rupture, nor degradation of the material at the crack tip. 相似文献
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Holly Warren Reece D. Gately Patrick O'Brien Robert Gorkin III Marc in het Panhuis 《Journal of Polymer Science.Polymer Physics》2014,52(13):864-871
The electrical impedance behavior of gellan gum (GG), GG–carbon nanotube, and GG–carbon nanofiber hydrogel composites is reported. It is demonstrated that the impedance behavior of these gels can be modeled using a Warburg element in series with a resistor. Sonolysis (required to disperse the carbon fillers) does not affect GG hydrogel electrical conductivity (1.2 ± 0.1 mS/cm), but has a detrimental effect on the gel's mechanical characteristics. It was found that the electrical conductivity (evaluated using impedance analysis) increases with increasing volume fraction of the carbon fillers and decreasing water content. For example, carbon nanotube containing hydrogels exhibited a six‐ to sevenfold increase in electrical conductivity (to 7 ± 2 mS/cm) at water content of 82%. It is demonstrated that at water content of 95 ± 2% the electrical behavior of multiwalled nanotube containing hydrogels transitions (percolates) from transport dominated by ions (owing to GG) to transport dominated by electrons (owing to the carbon nanotube network). © 2014 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2014 , 52, 864–871 相似文献
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