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EP Sheretov VS Gurov MV Dubkov OV Korneeva 《Rapid communications in mass spectrometry : RCM》1999,13(16):1699-1702
In this article we compare the classical monopole mass filter of von Zahn and the monopole mass filter with a hyperbolic V-shaped electrode. The experimental results and those of computer simulation for both mass spectrometers are presented. We show that the replacement of a conventional 90 degrees V-shaped electrode by an electrode with a hyperbolic profile substantially improves the peak shape of any given mass, and increases the mass resolution by a factor of 3-4 and the abundance sensitivity by a factor of 100. The potential of high analytical performance combined with electroforming techniques for electrode manufacture indicate future practical uses of such instruments. Copyright 1999 John Wiley & Sons, Ltd. 相似文献
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DC magnetization, neutron depolarization and neutron diffraction (with both polarized and unpolarized neutrons) measurements
have been reported for the Co1.1−x
Zn
x
Ge0.1Fe1.2O1 spinels with x=0.5, 0.6 and 0.7. Neutron depolarization and neutron diffraction measurements confirm the presence of a long range ferrimagnetic
ordering of the local canted spins in these ferrite samples. The observed features of low field magnetization have been explained
under the framework of thermally activated domain wall movement of ferrimagnetic arrangement of local canted spins. An important
role of magnetic anisotropy (due to the presence of Co2+ ions) in establishing the magnetic ordering and domain kinetics in these ferrites has been observed. 相似文献
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MV Muftakhov YV Vasil'ev VA Mazunov 《Rapid communications in mass spectrometry : RCM》1999,13(12):1104-1108
Appearance energies of [M-H](-) ions from carbonyl compounds R-CO-R' (R,R' = H, CH(3), NH(2), OH) have been measured by means of negative ion mass spectrometry in resonant electron capture mode. Values of electron affinity of the corresponding radicals, CH(2)&dbond;C(X)O, NH&dbond;C(X)O and O&dbond;C(X)O, have been determined. Copyright 1999 John Wiley & Sons, Ltd. 相似文献
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Tovmasyan AG Rajic Z Spasojevic I Reboucas JS Chen X Salvemini D Sheng H Warner DS Benov L Batinic-Haberle I 《Dalton transactions (Cambridge, England : 2003)》2011,40(16):4111-4121
Cationic Mn(III) N-alkylpyridylporphyrins (MnPs) are potent SOD mimics and peroxynitrite scavengers and diminish oxidative stress in a variety of animal models of central nervous system (CNS) injuries, cancer, radiation, diabetes, etc. Recently, properties other than antioxidant potency, such as lipophilicity, size, shape, and bulkiness, which influence the bioavailability and the toxicity of MnPs, have been addressed as they affect their in vivo efficacy and therapeutic utility. Porphyrin bearing longer alkyl substituents at pyridyl ring, MnTnHex-2-PyP(5+), is more lipophilic, thus more efficacious in vivo, particularly in CNS injuries, than the shorter alkyl-chained analog, MnTE-2-PyP(5+). Its enhanced lipophilicity allows it to accumulate in mitochondria (relative to cytosol) and to cross the blood-brain barrier to a much higher extent than MnTE-2-PyP(5+). Mn(III) N-alkylpyridylporphyrins of longer alkyl chains, however, bear micellar character, and when used at higher levels, become toxic. Recently we showed that meta isomers are ~10-fold more lipophilic than ortho species, which enhances their cellular accumulation, and thus reportedly compensates for their somewhat inferior SOD-like activity. Herein, we modified the alkyl chains of the lipophilic meta compound, MnTnHex-3-PyP(5+) via introduction of a methoxy group, to diminish its toxicity (and/or enhance its efficacy), while maintaining high SOD-like activity and lipophilicity. We compared the lipophilic Mn(III) meso-tetrakis(N-(6'-methoxyhexyl)pyridinium-3-yl)porphyrin, MnTMOHex-3-PyP(5+), to a hydrophilic Mn(III) meso-tetrakis(N-(2'-methoxyethyl)pyridinium-3-yl)porphyrin, MnTMOE-3-PyP(5+). The compounds were characterized by uv-vis spectroscopy, mass spectrometry, elemental analysis, electrochemistry, and ability to dismute O(2)˙(-). Also, the lipophilicity was characterized by thin-layer chromatographic retention factor, R(f). The SOD-like activities and metal-centered reduction potentials for the Mn(III)P/Mn(II)P redox couple were similar-to-identical to those of N-alkylpyridyl analogs: log k(cat) = 6.78, and E(1/2) = +68 mV vs. NHE (MnTMOHex-3-PyP(5+)), and log k(cat) = 6.72, and E(1/2) = +64 mV vs. NHE (MnTMOE-3-PyP(5+)). The compounds were tested in a superoxide-specific in vivo model: aerobic growth of SOD-deficient E. coli, JI132. Both MnTMOHex-3-PyP(5+) and MnTMOE-3-PyP(5+) were more efficacious than their alkyl analogs. MnTMOE-3-PyP(5+) is further significantly more efficacious than the most explored compound in vivo, MnTE-2-PyP(5+). Such a beneficial effect of MnTMOE-3-PyP(5+) on diminished toxicity, improved efficacy and transport across the cell wall may originate from the favorable interplay of the size, length of pyridyl substituents, rotational flexibility (the ortho isomer, MnTE-2-PyP(5+), is more rigid, while MnTMOE-3-PyP(5+) is a more flexible meta isomer), bulkiness and presence of oxygen. 相似文献
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Pasquini C de Aquino EV das Virgens Reboucas M Gonzaga FB 《Analytica chimica acta》2007,600(1-2):84-89
A flow–batch system was constructed and evaluated to perform coulometric titrations with biamperometric end point detection. The flow section of the system is employed for sampling by injecting a sample volume (50–300 μL) in a flow injection-like system. About 1.5 mL of a suitable carrier solution is delivered by a peristaltic pump in order to quantitatively transfer the sample to the system titration cell (2.0 mL total inner volume). The carrier contains the coulometric precursor for the titrant species. The cell contains two pairs of platinum electrodes used for coulometric generation of reagent and biamperometric detection and is actively stirred. The titrant species is generated and the titration is performed by the usual batch procedure with the excess of titrant being detected by biamperometry following the analysis of the titration curve. System operation is computer controlled and all operations are automated, including titration curve analysis and cell cleaning after the titration is ended. The system is characterized by its robustness because its operation does not depend on flow rates, and the work using coulometric methods which generate gases at the counter-electrode is not troublesome. The flow–batch system has been evaluated for determination of bromine index and bromine number (relative to the total reactive olefin content) in petrochemicals according to an ASTM procedure. Typical precision (R.S.D.) is between 0.5 and 6% for different petrochemicals whose bromine number/index vary from 1000 to 10 mg of bromine per 100 g of sample, respectively. Recoveries for standard additions are between 92 and 123% for 10 mg of Br2 per 100 g increments and 98 to 101% for 100 mg per 100 g increments. Accuracy of the proposed system was evaluated against results obtained by the standard ASTM with no significant difference detected at 95% confidence level. 相似文献
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Pirovani CP Carvalho HA Machado RC Gomes DS Alvim FC Pomella AW Gramacho KP Cascardo JC Pereira GA Micheli F 《Electrophoresis》2008,29(11):2391-2401
Preparation of high-quality proteins from cacao vegetative organs is difficult due to very high endogenous levels of polysaccharides and polyphenols. In order to establish a routine procedure for the application of proteomic and biochemical analysis to cacao tissues, three new protocols were developed; one for apoplastic washing fluid (AWF) extraction, and two for protein extraction--under denaturing and nondenaturing conditions. The first described method allows a quick and easy collection of AWF--using infiltration-centrifugation procedure--that is representative of its composition in intact leaves according to the smaller symplastic contamination detected by the use of the hexose phosphate isomerase marker. Protein extraction under denaturing conditions for 2-DE was remarkably improved by the combination of chemically and physically modified processes including phenol, SDS dense buffer and sonication steps. With this protocol, high-quality proteins from cacao leaves and meristems were isolated, and for the first time well-resolved 1-DE and 2-DE protein patterns of cacao vegetative organs are shown. It also appears that sonication associated with polysaccharide precipitation using tert-butanol was a crucial step for the nondenaturing protein extraction and subsequent enzymatic activity detection. It is expected that the protocols described here could help to develop high-level proteomic and biochemical studies in cacao also being applicable to other recalcitrant plant tissues. 相似文献