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Ratthaphol Charlermroj Michalina Oplatowska Oraprapai Gajanandana Orawan Himananto Irene R. Grant Nitsara Karoonuthaisiri Christopher T. Elliott 《Mikrochimica acta》2013,180(7-8):643-650
We have made a comparison of (a) different surface chemistries of SPR sensor chips (such as carboxymethylated dextran and carboxymethylated C1) and (b) of different assay formats (direct, sandwich and subtractive immunoassay) in order to improve the sensitivity of the determination of the model bacteria Acidovorax avenae subsp. citrulli (Aac). The use of the carboxymethylated sensor chip C1 resulted in a better sensitivity than that of carboxymethylated dextran CM5 in all the assay formats. The direct assay format, in turn, exhibits the best sensitivity. Thus, the combination of a carboxymethylated sensor chip C1 with the direct assay format resulted in the highest sensitivity for Aac, with a limit of detection of 1.6?×?106 CFU mL-1. This SPR immunosensor was applied to the detection of Aac in watermelon leaf extracts spiked with the bacteria, and the lower LOD is 2.2?×?107 CFU mL?1. Figure
Possible strategies to improve the surface plasmon resonance-based immmunodetection of bacterial cells Acidovorax avenae subsp. citrulli (Aac) was used as a model pathogen. Two different sensor surfaces (carboxymethylated dextran CM5 and carboxymethylated C1) were compared. Direct detection, sandwich system and subtractive assay were investigated. The combination of a C1 chip with the direct assay format resulted in the highest sensitivity for Aac, with a limit of detection of 1.6*106 CFU mL?1 相似文献
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