Quantification of Major Bioactive Withanolides in Withania somnifera (Ashwagandha) Roots by HPTLC for Rapid Validation of Ayurvedic Products

JPC – Journal of Planar Chromatography – Modern TLC - The high-performance thin-layer chromatography (HPTLC) method has been developed for the simultaneous quantification of withaferine...     

Advanced polymeric dendrimers in the management of Alzheimer's disease

In recent years, advanced polymeric dendrimers have emerged as a promising avenue for AD management. Dendrimers are highly branched, three-dimensional macromolecules with precise nanoarchitectures, making them ideal candidates for the delivery of therapeutic agents and diagnostic tools. Their unique properties, such as well-defined size, multifunctionality, and controlled surface chemistry, allow for the design of targeted and highly efficient drug delivery systems and diagnostic probes. This review aims to provide a comprehensive overview of the potential applications of advanced polymeric dendrimers in the management of Alzheimer's disease. We explored their role in drug delivery, diagnostics, and other therapeutic interventions for AD. Additionally, we will delve into the challenges and opportunities in utilizing dendrimers as a key player in the battle against this devastating disease. The review will begin by discussing the current state of Alzheimer's disease, including its pathological features, clinical manifestations, and existing treatment strategies. It will then transition to an in-depth examination of polymeric dendrimers, highlighting their structural characteristics, synthesis methods, and biocompatibility. Subsequently, the review will delve into the various ways in which dendrimers can be tailored for AD management, including drug encapsulation and delivery, enhanced blood–brain barrier penetration, and targeted diagnostic imaging. Furthermore, we explored the potential benefits of dendrimer-based therapies, such as improved drug efficacy, reduced side effects, and enhanced patient compliance. The review will also address the challenges associated with dendrimer-based approaches, including toxicity concerns, regulatory hurdles, and the need for rigorous clinical evaluation.     

Synthesis and characterization of Mannich bases of lawsone and their anticancer activity

Abstract

Natural and synthetic naphthoquinones are known for a large number of biological activities. Lawsone (2-hydroxy-1, 4-naphthoquinone) is a simplest naturally occurring compound obtained from dried henna (Lawsonia inermis) leaves. In literature, some lawsone derivatives have been reported to exhibit anticancer activity. Hence, a clean and facile one-pot protocol was developed for the synthesis of new aminonaphthoquinones derived from lawsone by three-component Mannich reaction, at room temperature for potential anti-cancer application. Herein we present a small library of Mannich bases with different amines and aromatic aldehydes with moderate to high yield. Synthesized compounds were characterized using various spectroscopic techniques. The anticancer activity (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay) along with nuclear morphology assessment (4′,6-diamidino-2-phenylindole or DAPI staining), apoptosis assessment (acridine orange/ ethidium bromide staining), hemolysis and DNA ladder assay evaluated on human liver carcinoma cell line HepG2 are presented.     

Design,synthesis and in vitro bioactivity of mixed ligand Ru(II) complexes bearing the fluoroquinolone antibacterial agents

Transition Metal Chemistry - Mixed ligand Ru(II) phenanthroline complexes of the type [Ru(1,10-phen)2Flq]ClO4 (RPFlq-1-3) and “piano-stool”-type Ru(II) arene complexes...     

Synthesis, characterization, DNA binding and cytotoxicity studies of moxifloxacinato complexes

Five metal complexes of the third-generation quinolone antibacterial agent moxifloxacin with Cu(II), Fe(III), Mn(II), Ni(II) and VO(II) have been synthesized and characterized by physicochemical and spectroscopic techniques. In these complexes, moxifloxacin acts as a bidentate deprotonated ligand bound to the metal through ketone and carboxylate oxygens. The interactions between the metal complexes and calf thymus DNA have been studied by UV?CVis, circular dichroism and cyclic voltammetry. Fluorescence competitive binding studies with ethidium bromide (EB) demonstrate the ability of the complexes to displace the EB bound to DNA. The cytotoxicities of the complexes have been evaluated on A549 cells by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) method. [Cu(MFL)₂(H₂O)₂] shows the highest anticancer potency. The apoptosis-inducing activity was assessed by acridine orange/ethidium bromide staining assay.     

Estimation of Antioxidant Potential of Individual Components Present in Complex Mixture of Withania somnifera (Ashwagandha) Root Fraction by Thin-Layer Chromatography—2,2-Diphenyl-1-Picrylhdrazyl Method

JPC – Journal of Planar Chromatography – Modern TLC - A thin-layer chromatography–2,2-diphenyl-1-picrylhdrazyl (TLC—DPPH) method has been developed for the quantification of...