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1.
Background
The completion of several genome-sequencing projects has increased our need to assign functions to newly identified genes. The presence of a specific protein domain has been used as the determinant for suggesting a function for these new genes. In the case of proteins that are predicted to interact with mRNA, most RNAs bound by these proteins are still unknown. In yeast, several protocols for the identification of protein-protein interactions in high-throughput analyses have been developed during the last years leading to an increased understanding of cellular proteomics. If any of these protocols or similar approaches shall be used for the identification of mRNA-protein complexes, the integrity of mRNA is a critical factor. 相似文献2.
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Qun Li Andreas Ricoeur Meinhard Kuna 《Archive of Applied Mechanics (Ingenieur Archiv)》2011,81(6):685-700
The axisymmetric problem of a penny-shaped crack embedded in an infinite three-dimensional (3D) piezoelectric body is considered.
A general formulation of Coulomb traction on the crack surfaces can be obtained based on thermodynamical considerations of
electromechanical systems. Three-dimensional electroelastic solutions are derived by the classical complex potential theory
when Coulomb traction is taken into account and the poling direction of piezoelectric body is perpendicular to the crack surfaces.
Numerical results show that the magnitude of Coulomb tractions can be large, especially when a large electric field in connection
with a small mechanical load is applied. Unlike the traditional traction-free crack model, Coulomb tractions induced by an
applied electric field influence the Mode I stress intensity factor for a penny-shaped crack in 3D piezoelectric body. Moreover,
compared to the current model, the traditional traction-free crack model always overestimates the effect of the applied electric
load on the field intensity factors and energy release rates, which has consequences for 3D piezoelectric fracture mechanics. 相似文献
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Christian Heller Niklas Pucher Bernhard Seidl Kadiriye Kalinyaprak‐Icten Gerald Ullrich Ladislav Kuna Valentin Satzinger Volker Schmidt Helga C. Lichtenegger Jürgen Stampfl Robert Liska 《Journal of polymer science. Part A, Polymer chemistry》2007,45(15):3280-3291
Intrigued by the good performance of 1,5‐diphenylpenta‐1,4‐diyn‐3‐one ( DPD ) as photoinitiator for radical polymerization we prepared and investigated several donor substituted derivatives. UV‐Vis spectroscopy revealed a gradual red‐shift of λmax and higher extinction in the order of the donor capability. A methoxy‐substituted derivative ( O‐DPD ) exhibited significant photoinitiation activity in photo‐DSC experiments. Steady state photolysis experiments showed decreased decomposition rates with increasing donor capability. A dimethylamino derivative N‐DPD was even photostable under these conditions. Because of to the D‐π‐A‐π‐D system of these compounds two‐photon induced 3D photopolymerization experiments were performed and N‐DPD showed outstanding performance compared to often applied single photon initiators. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 45: 3280–3291, 2007 相似文献
7.
The energy density criterion is employed to characterize the crack initiation of pre-cracked specimens. Three-point-bending specimens are used with normalized crack length varying from 0.1 to 0.5. The specimens are made of German steel StE690. The results show that the local energy density increases with almost the same rate with crack extension as the specimen geometry changes. This indicates that the strain energy density can be used to characterize the onset of crack initiation for pre-cracked specimens. 相似文献
8.
Delamination of thermally sprayed corrosion protection coatings as a consequence of thermo-mechanical fatigue is investigated. This study focusses on the modelling of interfacial damage initiation and evolution under cyclic loading with the help of a cohesive zone model. The presented model features a slight non-linearity at unloading from the exponential “damage locus” as well as cyclic damage accumulation restricted to (re)loading conditions. Additionally, an endurance limit is introduced indicating the maximum sustainable traction for an infinite number of load cycles. The capability of the model is demonstrated. (© 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献
9.
This paper presents a plane strain gradient elasticity model together with a strength criterion based on the maximum hoop stress. It is developed by higher order homogenization based on a cylindrical RVE containing a void and is implemented as mixed–type finite element formulation into the FE–code Abaqus. Numerical simulations are performed in order to study the size effect related to the onset of failure of specimens of porous elastic material. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献
10.
S. Goldstein T. Kuna J. L. Lebowitz E. R. Speer 《Journal of statistical physics》2017,166(3-4):765-782
We investigate the following questions: Given a measure \(\mu _\Lambda \) on configurations on a subset \(\Lambda \) of a lattice \(\mathbb {L}\), where a configuration is an element of \(\Omega ^\Lambda \) for some fixed set \(\Omega \), does there exist a measure \(\mu \) on configurations on all of \(\mathbb {L}\), invariant under some specified symmetry group of \(\mathbb {L}\), such that \(\mu _\Lambda \) is its marginal on configurations on \(\Lambda \)? When the answer is yes, what are the properties, e.g., the entropies, of such measures? Our primary focus is the case in which \(\mathbb {L}=\mathbb {Z}^d\) and the symmetries are the translations. For the case in which \(\Lambda \) is an interval in \(\mathbb {Z}\) we give a simple necessary and sufficient condition, local translation invariance (LTI), for extendibility. For LTI measures we construct extensions having maximal entropy, which we show are Gibbs measures; this construction extends to the case in which \(\mathbb {L}\) is the Bethe lattice. On \(\mathbb {Z}\) we also consider extensions supported on periodic configurations, which are analyzed using de Bruijn graphs and which include the extensions with minimal entropy. When \(\Lambda \subset \mathbb {Z}\) is not an interval, or when \(\Lambda \subset \mathbb {Z}^d\) with \(d>1\), the LTI condition is necessary but not sufficient for extendibility. For \(\mathbb {Z}^d\) with \(d>1\), extendibility is in some sense undecidable. 相似文献