Synthesis,characterization, DNA binding,antibacterial, and antioxidant activity of new bis-phthalimides

New bis-phthalimides were synthesized by 2: 1 condensation of phthalic anhydride and tetrachlorophthalic anhydride with 1,2,4-triazole-3,5-diamine, pyridine-2,6-diamine, and 4-hydroxypyrimidine- 2,6-diamine. The synthesized compounds were characterized by elemental analyses and IR, ¹H NMR, and mass spectra. Their interaction with calf thymus DNA (ct-DNA) was studied by UV-Vis spectrophotometry, cyclic voltammetry, and viscosity measurements, which revealed intercalative mode of binding to ct-DNA. Antibacterial activity of the synthesized compounds against Escherichia coli and Streptococcus mutans was assessed in vitro by the agar well diffusion method. The antioxidant activity of these compounds was estimated by DPPH assay.     

Synthesis,crystal structures and spectral characterization of Cu(II) and Mn(II) complexes of 4-hydroxy-3-methoxybenzaldehyde: antioxidant properties and molecular docking studies

[Cu(L)₂(H₂O)₂] (1) and [Mn(L)₂(H₂O)₂] (2) (HL = 4-hydroxy-3-methoxybenzaldehyde) were synthesized and characterized using elemental, spectral (FTIR, ESI-MS, UV–visible, fluorescence and EPR), thermal, cyclic voltammetric, powder, and single crystal X-ray crystallographic studies. Spectral and X-ray data ascertained the structural features, binding modes of ligand and distorted octahedral geometry around the metal ions. Cyclic voltammetric studies confirmed the formation of a quasi reversible redox couple in solution. Crystal structure analysis of 1 and 2 reveal the presence of non-covalent interactions, resulting in a 1-D polymeric chain. Antioxidant properties (using DPPH and hydrogen peroxide assay) and molecular docking studies (using 1BNA) are also examined. The binding free energies (calculated from docked models), ?270 (1) and ?295 kJ mol^?1 (2), suggest that the complexes reasonably bind to DNA, and the DNA-binding affinity of 2 is stronger than that of 1.