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JPC – Journal of Planar Chromatography – Modern TLC - A new quantitative method using thin-layer chromatography silica gel 60F254 plates as the stationary phase and the mobile phase... 相似文献
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Kha Tram Pushpinder Kanda Bruno J. Salena Shuangyan Huan Yingfu Li 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2014,126(47):13013-13016
Microbial pathogens pose serious threats to public health and safety, and results in millions of illnesses and deaths as well as huge economic losses annually. Laborious and expensive pathogen tests often represent a significant hindrance to implementing effective front‐line preventative care, particularly in resource‐limited regions. Thus, there is a significant need to develop low‐cost and easy‐to‐use methods for pathogen detection. Herein, we present a simple and inexpensive litmus test for bacterial detection. The method takes advantage of a bacteria‐specific RNA‐cleaving DNAzyme probe as the molecular recognition element and the ability of urease to hydrolyze urea and elevate the pH value of the test solution. By coupling urease to the DNAzyme on magnetic beads, the detection of bacteria is translated into a pH increase, which can be readily detected using a litmus dye or pH paper. The simplicity, low cost, and broad adaptability make this litmus test attractive for field applications, particularly in the developing world. 相似文献
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Kaur Pushpinder Chaudhary Abha Katiyar Arti Singh Bikram Gopichand Singh Rakesh D. 《平面色谱法杂志一现代薄层色谱法》2012,25(5):415-419
JPC – Journal of Planar Chromatography – Modern TLC - A reverse phase high-performance thin-layer chromatography (RPHPTLC) method was developed for determination of vitexin, hyperoside,... 相似文献
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A novel approach for the direct C-4 arylation of N-methyl-1,2,3,4-tetrahydroisoquinolines by nucleophilic addition of β-aminocarbanions to benzynes is described which provides a one-pot procedure for synthesis of the title compounds. 相似文献
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JPC – Journal of Planar Chromatography – Modern TLC - A reversed-phase high-performance thin-layer chromatography (RP-HPTLC) method was developed for the determination of flavonoids... 相似文献
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Ranking of fuzzy numbers play an important role in decision making, optimization and forecasting etc. Fuzzy numbers must be
ranked before an action is taken by a decision maker. In this paper, with the help of several counter examples, it is proved
that ranking method proposed by Chen and Chen (Expert Systems with Applications 36 (3): 6833) is incorrect. The main aim of
this paper is to propose a new approach for the ranking of generalized trapezoidal fuzzy numbers. The proposed ranking approach
is based on rank and mode so it is named as an RM approach. The main advantage of the proposed approach is that the proposed
approach provides the correct ordering of generalized and normal trapezoidal fuzzy numbers and also the proposed approach
is very simple and easy to apply in the real life problems. It is shown that proposed ranking function satisfies all the reasonable
properties of fuzzy quantities proposed by Wang and Kerre (Fuzzy Sets and Systems 118 (3): 375). 相似文献
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A new series of benzocycloheptene amino vinyl bromide derivatives (9a-9m) were synthesized from isomeric mixture of himachalenes through two-step synthesis. The unusual structure of benzocycloheptene amino vinyl bromide derivative (9a) was confirmed by NMR and X-ray crystallography analyses. The newly synthesized amino vinyl bromide derivatives of benzocycloheptene were further evaluated for their antidepressant activities. The compound 9c had shown significant reduction in the immobility period. 相似文献
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Chaudhary A Kaur P Kumar N Singh B Awasthi S Lal B 《Natural product communications》2011,6(11):1617-1620
Albizia species have been shown to have anti-inflammatory and anti-allergic properties. However, efficient analytical methods for identification of their active constituents are still lacking. Ultra-performance liquid chromatography coupled with electrospray ionization-quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS) was used to study the phenolic composition of the ethanolic extracts of different parts (flowers, leaves, pods and bark) of A. chinensis. In addition, the antioxidant activity of the ethanolic extracts was evaluated by the 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical cation scavenging methods. Four compounds were isolated from the ethanolic extract of the flowers and characterized by 1H and 13C NMR spectroscopy as quercetin-3-O-rhamnoside, quercetin, quercetin-3-O-arabinofuranoside, and myricetin-3-O-rhamnoside. Separation and quantification of the phenolics was accomplished using a reversed-phase BEH C18 column with the mobile phase of methanol-water (0.05% formic acid), and detection wavelengths of 360 and 254 nm. 相似文献