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Pereira-Meirelles Fatima Ventura Rocha-LeãO Maria Helena Miguez Anna Geraldo Lippel Santť 《Applied biochemistry and biotechnology》1997,(1):73-85
Although Upases have been intensively studied, some aspects of enzyme production like substrate uptake, catabolite repression,
and enzyme stability under long storage periods are seldom discussed in the literature. This work deals with the production
of lipase by a new selected strain ofCandida lipolytica. Concerning nutrition, it was observed that inorganic nitrogen sources were not as effective as peptone, and that oleic acid
or triacylglycerides (TAG) were essential carbon sources. Repression by glucose and stimulation by oleic acid and long chain
TAG (triolein and olive oil) were observed. Extracellular lipase activity was only observed at high levels at late stationary
phase, whereas intracellular lipase levels were constant and almost undetectable during the cultivation period, suggesting
that the produced enzyme was attached to the cell wall, mainly at the beginning of cultivation. The crude lipase produced
by this yeast strain shows the following optima conditions: pH 8.0–10.0, temperature of 55°C. Moreover, this preparation maintains
its full activity for at least 370 d at 5°C. 相似文献
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