阿里红多糖的气相色谱和红外光谱

采用乙醇沉淀得到阿里红多糖(FoP),用Sevag法除去蛋白后采用气相色谱(GC)和红外光谱(IR)对得到的阿里红多糖进行分析.结果表明,阿里红多糖中含有鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖,其摩尔比为0.2:0.29∶0.41∶0.77∶0.70∶ 1.16.红外光谱分析阿里红多糖中存在吡喃环.     

区间数及在控制系统中的应用

在生产过程中,有些控制系统中的参数不是常数而是一个区间时,与常规的方法有较大的不同.本文以一阶系统为例,在MATLAB环境下用INTLAB软件编程建立运行区间系统,获得采用PI调节器进行校正后其系统的输出响应,并用仿真结果的变化来确定区间系统的最优控制参数.     

CCR4 expressing cells cultured adherently on a capillary wall and formaldehyde fixed as the stationary phase for ligand screening by ACE

We have developed an on‐line screening method for CC chemokine receptor 4 (CCR4) ligands, in which the whole cells expressed with CCR4 were cultured adherently and immobilized on the inner wall of the capillary as the stationary phase for the first time. Moreover, in this method it is unnecessary to isolate and purify the target receptors from cell membranes. Therefore, it is possible to almost completely preserve the native conformation of the target receptors. The binding activities of the immobilized CCR4 did not change. A known antagonist of CCR4, compound A, was employed to validate the bioactivity of the cell layer and stability of this method. The intraday, interday, and batch‐to‐batch reproducibilities were investigated (RSD ≤ 13.9%). Nonlinear chromatography was used to calculate the binding constant between the compound A and CCR4 (6.4 × 10⁴/M, RSD = 4.96%). Using this method, the qualitative and quantitative characterizations of 23 computer‐aided drug design compounds were achieved and the kinetic parameters (K, k_a, k_d, and k′) were obtained by nonlinear chromatography. Three active compounds were screened out, which also showed activity in chemotaxis inhibition assay. The experimental results show that this method is simple, sensitive, and efficient for drug screening. Moreover, it offered a novel way to detect the nonspecific interactions between ligands and cell membrane.     

飞秒激光与等离子体相互作用中自生磁场的热电机制

采用理论分析和数值模拟研究了考虑相对论效应的自生磁场及其产生机制,给出了自生磁场的解析表达式,得到了温度梯度和密度梯度的非共线性所引起的自生磁场空间分布的时间演化关系。数值结果表明,当峰值强度为1019 W/cm2的激光作用于凹形靶前表面时,在等离子体表面领域观察到的自生磁场最大值为51102 T量级,与实验测量结果相符合。