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T. Melvin S. Cunniffe D. Papworth T. Roldan-Arjona P. O'Neill 《Photochemistry and photobiology》1997,65(4):660-665
Abstract— Irradiation of aqueous solutions of plasmid DNA (pUC18) at pH 7.6 with 193 nm laser light results in low yields of prompt single strand breakage (air-saturated sample φssb = [1.5 ± 0.1] ± 10−4 , argon-saturated sample φssb = [0.9 ± 0.1] ± 10−4 ). Treatment of the irradiated DNA samples with Escherichia coli formamidopyrimi-dine-DNA glycosylase (Fpg) protein results in an approximate 20-fold increase in the yield of single strand breakage (air-saturated sample φfpg = [33.1 ± 3.1] ± 10−4 , argon-saturated sample φfpg = [23.8 ± 2.6] × 10 4 ). This result indicates that 193 nm light induces other modification) (most likely of the purine moieties) that are 20 times more abundant than prompt strand breakage within the DNA matrix. 相似文献
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CM Silva MF Duarte ML Mira MH Florêncio K Versluis AJ Heck 《Rapid communications in mass spectrometry : RCM》1999,13(12):1098-1103
Fast atom bombardment, combined with high-energy collision-induced tandem mass spectrometry, has been used to investigate gas-phase metal-ion interactions with captopril, enalaprilat and lisinopril, all angiotensin-converting enzyme inhibitors.Suggestions for the location of metal-binding sites are presented. For captopril, metal binding occurs most likely at both the sulphur and the nitrogen atom. For enalaprilat and lisinopril, binding preferably occurs at the amine nitrogen. Copyright 1999 John Wiley & Sons, Ltd. 相似文献
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A procedure is explained to determined the amount of several pairs of diametrical loads applied to the outside boundary of
a ring when stresses at selected points of the inside or outside boundaries are known. Coefficients of influence are used,
following an approach similar to the one presented in a previous paper. Examples of application are given and the possible
increase in precision is shown when the number of points of measurements is larger than the number of loads to be determined. 相似文献
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