排序方式: 共有80条查询结果,搜索用时 0 毫秒
1.
Reaction between gadolinium(III) and 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (5- Br-PADAP) was studied for delineating optimal conditions for complexation. This reagent can be used for the spectrophotometric determination of Gd(III) in concentrations ranging from 0.04 to 1.2 ppm (a = 1.76(+/- 0.03) x 10(5) (1.(-1) mole(-1). cm). The reaction takes place at a pH between 9.2 and 11.6. In the presence of Triton X-100 this complex is soluble in water. In order to overcome difficulties caused by the presence of other lanthanides, an ion exchange chromatographic technique was used. 相似文献
2.
3.
8-Quinolinol (oxine) and 2-(2-(5 chloropyridylazo)-5-dimethylamino)-phenol (5ClDMPAP) were immobilized on the non ionic sorbents Amberlite XAD-4 and XAD-7. These loaded resins were used for the preconcentration of Ce, La and Pr. High preconcentration factors were obtained in each case. After the retention of these rare earths, the resins were measured as thin films by x-ray fluorescence spectrometry. Up to 50 ppm of REEs can be retained on these thin films. 相似文献
4.
C. Zucchi M. Forneris L. Martínez R. Olsina E. Marchevsky 《Analytical and bioanalytical chemistry》1998,360(1):128-130
A selective and precise spectrophotometric determination of vanadium(V) is performed after preceding extraction with N-benzoyl-N-phenylhydroxylamine (BPHA). The color is developed in a water-ethanol solution with hydrogen peroxide and 2-(5-chloro-2-pyridylazo)-5-dimethylaminophenol (5-Cl-DMPAP). The molar absorptivity at 588 nm is (6.57 ± 0.05) × 104 L mol–1 cm–1 at pH 2.1. The method permits the determination of vanadium (V) at trace levels in the presence of large amounts of other ions. It is applied to the determination of vanadium in aluminium (analytical reagent grade) and in human hair. High accuracy and precision is obtained. 相似文献
5.
Chelating resins prepared by sorption of 2-(5-bromo-2-pyridylazo)-5-(diethylamino)phenol (5-BrPADAP) on macroporous resins
(Amberlite XAD-4 and XAD-7) were characterized. The adsorption properties (amount of chelating agent adsorbed per gram of
resin, sorption kinetics, retention capacity, etc.) and the thermodynamic quantities of each adsorption process were determined.
The retention of some rare earth elements (e.g. Er, Yb and Lu) on these chelating resins was studied in order to preconcentrate
them for their determination by X-Ray fluorescence spectrometry.
Received: 7 September 1995/Revised: 2 January 1996/Accepted: 10 January 1996 相似文献
6.
Patricia W. Stege Germán A. Messina Guillermo Bianchi Roberto A. Olsina 《Journal of fluorescence》2010,20(2):517-523
Enzyme activities can provide indication for quantitative changes in soil organic matter (SOM). It is known that the activities
of most enzymes increase as native SOM content reflecting larger microbial communities and stabilization of enzymes on humic
materials. β-Gucosidase (β-Glu) activities have been frequently used as indicators of changes in quantity and quality of SOM.
In this study we propose a simple and very sensitive method, which has lower limit of detection compared with classic spectrophotometric
method with the aim of determinate the β-Glu activity in soil samples using Fluorescein mono-β-D-glucopyranoside (FMGlc) as
a substrate. The fluorescein released by the enzymatic reaction was quantified by capillary electrophoresis-laser induced
fluorescence (CE-LIF) method. The background electrolyte (BGE) consisted in 40 mM phosphate buffer, pH 6. The LOD and LOQ
for fluorescein were 1.3 10−7 mg mL−1 and 6.4 10−6 mg mL−1, respectively. This work deals with the minimization of the mixture for the enzymatic reaction and with the optimization
conditions of CE separation. To the best of our knowledge, this is the first time that an enzymatic activity was detected
in soil using CE-LIF system. 相似文献
7.
Patricia W. Stege Germán A. Messina Guillermo Bianchi Roberto A. Olsina Julio Raba 《Analytical and bioanalytical chemistry》2010,397(3):1347-1353
Soil microorganisms and enzymes are the primary mediators of soil biological processes, including organic matter degradation,
mineralization, and nutrient recycling. They play an important role in maintaining soil ecosystem quality and functional diversity.
Moreover, enzyme activities can provide an indication of quantitative changes in soil organic matter. β-Glucosidase (β-Glu)
activity has been found to be sensitive to soil management and has been proposed as a soil quality indicator because it provides
an early indication of changes in organic matter status and its turnover. The aims of the present study were to test and use
a simple and convenient procedure for the assay of β-Glu activity in agricultural soil. The method described here is based
on the enzymatic degradation of cellobiose by β-Glu present in the soil sample and the subsequent determination of glucose
produced by the enzymatic reaction using screen-printed carbon electrodes modified with multiwalled carbon nanotubes (SPCE-CNT)
equipped with coimmobilized glucose oxidase and horseradish peroxidase enzymes. The potential applied to the SPCE-CNT detection
was −0.15 V versus a Ag/AgCl pseudo-reference electrode. A linear calibration curve was obtained in the range 2.7–11.3 mM
with a correlation coefficient. In the present study, an easy and effective SPCE-CNT-modified electrode allowed an improved
amperometric response to be achieved and this is attributed to the increased surface area upon electrode modification. 相似文献
8.
Pablo H. Pacheco Roberto Olsina Griselda Polla Luis D. Martinez Patricia Smichowski 《Microchemical Journal》2009,91(2):159-164
The adsorption behaviour of Cd onto the relative non-polar L-methionine was studied. To this end, L-met was immobilized on controlled pore glass (CPG), incorporated in a microcolumn and inserted in a flow injection system for Cd preconcentration from aqueous solutions. Binding constant of the system was calculated and it turned to be of 1.99, with sites capacity of n = 3.12. The ratio of Cd moles bound to L-met moles was calculated and it was 0.03:1 at pH 9.0. On-line breakthrough curves were used to study the effect of pH, analyte concentration and influent flow rate on Cd retention. A complementary pH study was added with a titration curve. Transient peak areas revealed that Cd stripping from the column occurred instantaneously. The system achieves an enrichment factor of 130, reaching a detection limit of 0.63 ng L− 1 when 10 mL of the solution were passed through the column. The method was successfully applied to Cd determination in the standard reference material (SRM), QC METAL LL2 metals in natural water, as a validation study. 相似文献
9.
Raúl A. Gil José A. Salonia José A. Gásquez Alejandro C. Olivieri Roberto Olsina Luis D. Martinez 《Microchemical Journal》2010,95(2):306-310
A novel method for the determination of Pb by on-line cloud point extraction coupled to inductively coupled plasma optical emission spectrometry with ultrasonic nebulization (USN–ICP OES) is presented. The cloud point system was formed in the presence of non-ionic micelles of polyethyleneglycolmono-p-nonylphenylether (PONPE 7.5) and was retained in a minicolumn filled with particles of PTFE. Since the micelles of PONPE were able to extract Pb from the solutions, the use of a complexing reagent was not necessary. Afterwards, the surfactant-rich phase containing the analyte was removed from the minicolumn with nitric acid and introduced into the ultrasonic nebulizer. A total enhancement factor of 150 was obtained for a preconcentration time of 3.3 min, with respect to the direct determination of Pb by conventional ICP OES. The values of the detection (3σ) and quantification (10σ) limits for the preconcentration of 10 mL of sample solution were 0.09 µg L− 1 and 0.2 µg L− 1 respectively. The precision, expressed as the relative standard deviation (RSD), for 10 replicate determinations at 5.0 µg L− 1 Pb level was 6.0%. Verification of the accuracy was carried out by analysis of two certified reference materials (NIST SRM 1640e and VKI QC Metal LL1). The method was successfully applied to the determination of Pb in drinking water samples. 相似文献
10.
An on-line flow injection (FI) preconcentration-electrothermal atomic absorption spectrometry (ETAAS) method is developed for trace determination of chromium in drinking water samples by sorption on a conical minicolumn packed with activated carbon (AC) at pH 5.0. The chromium was removed from the minicolumn with 1.0% (v/v) nitric acid. An enrichment factor (EF) of 35-fold for a sample volume of 10 ml was obtained. The detection limit (DL) value for the preconcentration method proposed was 3.0 ng l−1. The precision for 10 replicate determinations at the 0.5 μg l−1 Cr level was 4.0% relative standard deviation (R.S.D.), calculate with the peak heights obtained. The calibration graph using the preconcentration system for chromium was linear with a correlation coefficient of 0.9992 at levels near the detection limits up to at least 50 μg l−1. The method was successfully applied to the determination of Cr(III) and Cr(VI) in drinking water samples. 相似文献