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1.
采用沉淀法制备前驱体,通过不同温度合成了上转换发光材料Y2O2S∶Er3+,Yb3+,运用XRD,SEM和上转换发射光谱对其进行表征。结果表明,所合成的Y2O2S∶Er3+Yb3+属于六方晶系晶体,随着合成温度的升高,产物的粒径不断增大,上转换发射光强度逐渐增加。研究Y2O2S∶Er3+Yb3+的上转换发光过程,红光发射和绿光发射分别源于Er3+离子的4F9/2→4I15/2以及2H11/2→4I15/2,4S3/2→4I15/2能级跃迁。利用群论计算了晶场中Er3+离子的能级分裂数目。 相似文献
2.
Ondrej Stranik Daniela Iacopino Robert Nooney Colette McDonagh Brian D. MacCraith 《Journal of fluorescence》2010,20(1):215-223
In this paper, we describe a novel technique for depositing metal nanoparticles (NPs) on a planar substrate whereby the NPs
are micro-patterned on the surface by a simple stamp-printing procedure. The method exploits the attractive force between
negatively charged colloidal metal NPs and positively-charged polyelectrolyte layers which have been selectively deposited
on the surface. Using this technique, large uniform areas of patterned metal NPs, with different plasmonic properties, were
achieved by optimisation of the stamping process. We report the observation of unusual fluorescence emission from these structures.
The emission was measured using epifluorescence microscopy. Fluorescence lifetime behaviour was also measured. Furthermore,
the μ-patterned NPs exhibited blinking behaviour under 469 nm excitation and the fluorescence spectrum was multi-peaked. It
has been established that the fluorescence is independent of the plasmon resonance properties of the NPs. As well as optimising
the novel NP μ-patterning technique, this work discusses the origin and characteristics of the anomalous fluorescence behaviour
in order to characterise and minimise this unwanted background contribution in the use of metal NPs for plasmonic enhancement
of fluorescence for optical biochip applications. 相似文献
3.
本文主要研究如下含非线性梯度项的非强制拟线性椭圆方程\begin{equation*}\left \{\begin{array}{rl}-\text{div}(\frac{|\nabla u|^{p-2}\nabla u}{(1+|u|)^{\theta(p-1)}})+\frac{|u|^{p-2}u|\nabla u|^{p}}{(1+|u|)^{\theta p}}=\mu,~&x\in\Omega,\\ u=0,~&x\in\partial\Omega,\end{array}\right.\end{equation*} 弱解的存在性和不存在性, 其中$\Omega\subseteq\mathbb{R}^N(N\geq3)$ 是有界光滑区域, $1
相似文献
4.
Xavier Le Guével Robert Nooney Colette McDonagh Brian D. MacCraith 《Journal of solid state chemistry》2011,184(6):1545-1550
Recently, multifunctional silica nanoparticles have been investigated extensively for their potential use in biomedical applications. We have prepared sub-micron monodisperse and stable multifunctional mesoporous silica particles with a high level of magnetization and fluorescence in the near infrared region using an one-pot synthesis technique. Commercial magnetite nanocrystals and a conjugated-NIR-dye were incorporated inside the particles during the silica condensation reaction. The particles were then coated with polyethyleneglycol to stop aggregation. X-ray diffraction, N2 adsorption analysis, TEM, fluorescence and absorbance measurements were used to structurally characterize the particles. These mesoporous silica spheres have a large surface area (1978 m2/g) with 3.40 nm pore diameter and a high fluorescence in the near infrared region at λ=700 nm. To explore the potential of these particles for drug delivery applications, the pore accessibility to hydrophobic drugs was simulated by successfully trapping a hydrophobic ruthenium dye complex inside the particle with an estimated concentration of 3 wt%. Fluorescence imaging confirmed the presence of both NIR dye and the post-grafted ruthenium dye complex inside the particles. These particles moved at approximately 150 μm/s under the influence of a magnetic field, hence demonstrating the multifunctionality and potential for biomedical applications in targeting and imaging. 相似文献
5.
Ye Wang Jian-Qing Gao Feng Li Sang-Hon RI Wen-Quan Liang 《Colloid and polymer science》2006,285(2):233-238
In many cases, initial release of drugs from microparticles is undesirable. In the present study, Pluronic®F127, which shows thermosensitive characteristic, was used for controlling both the initial release and the sustained release of insulin from microspheres. Calcium-alginate insulin microspheres were prepared by emulsion technology and dispersed in a thermosensitive semisolid gel, the PF127. In vitro study demonstrated that PF127 could protect the activity of insulin in some extent and control the initial release and sustained release of insulin well. The results of in vivo hypoglycemic experiment carried out on diabetic rats also displayed a well correlation with in vitro release patterns. The results suggested that PF127 could sustain insulin release through a subcutaneous injection, and this material was available to deliver insulin aiming for prolonged and smooth hypoglycemic function. 相似文献
6.
P. JÓVÁRI 《Molecular physics》2013,111(11):1149-1156
The structure factor of liquid CS2 has been measured at ambient temperature by the 7C2 diffractometer at the Laboratoire Leon Brillouin?, Saclay, France. The result has been modelled by the reverse Monte Carlo (RMC) method. The only reported neutron diffraction measurement on liquid CSe2 has also been investigated in detail. In both cases initial configurations for the RMC runs have been obtained by molecular dynamics simulation using a simple soft sphere potential. It has been found that the main features of experimental results have been reproduced even by this choice suggesting that the structure of these systems is almost entirely described by the excluded volume. Examination of model size dependence of results has also been carried out revealing the importance of using large simulation boxes. 相似文献
7.
Robert Nooney Andrew Clifford Xavier LeGuevel Ondrej Stranik Colette McDonagh Brian D. MacCraith 《Analytical and bioanalytical chemistry》2010,396(3):1127-1134
In this work, we used a model assay system (polyclonal human IgG–goat antihuman IgG) to elucidate some of the key factors
that influence the analytical performance of bioassays that employ metal-enhanced fluorescence (MEF) using silver nanoparticles
(NPs). Cy5 dye was used as the fluorescent label, and results were compared with a standard assay performed in the absence
of NPs. Two sizes of silver NPs were prepared with respective diameters of 60 ± 10 and 149 ± 16 nm. The absorption spectra
of the NPs in solution were fitted accurately using Mie theory, and the dipole resonance of the 149-nm NPs in solution was
found to match well with the absorption spectrum of Cy5. Such spectral matching is a key factor in optimizing MEF. NPs were
deposited uniformly and reproducibly on polyelectrolyte-coated polystyrene substrates. Compared to the standard assay performed
without the aid of NPs, significant improvements in sensitivity and in limit of detection (LOD) were obtained for the assay
with the 149-nm NPs. An important observation was that the relative enhancement of fluorescence increased as the concentration
of antigen increased. The metal-assisted assay data were analyzed using standard statistical methods and yielded a LOD of
0.086 ng/mL for the spectrally matched NPs compared to a value of 5.67 ng/mL obtained for the same assay in the absence of
NPs. This improvement of ∼66× in LOD demonstrates the potential of metal-enhanced fluorescence for improving the analytical
performance of bioassays when care is taken to optimize the key determining parameters.
相似文献
8.
李光云 《浙江大学学报(理学版)》2010,37(4)
基于Bézier曲线升阶的思想,构造了带多个形状参数的Bézier曲线,它具有与Bézier曲线相同的性质.在控制顶点不变的情况下,可通过改变多个形状参数的取值调整曲线的形状.n次Bézier曲线是n次带多个形状参数的Bézier曲线的一个特例,多个形状参数可使曲线变化更灵活. 相似文献
9.
10.
Gubala V Lynam CC Nooney R Hearty S McDonnell B Heydon K O'Kennedy R MacCraith BD Williams DE 《The Analyst》2011,136(12):2533-2541
In this article, we report on poly(amidoamine) dendrimers (PAMAM) as coupling agents for recombinant single-chain (ScFv) antibodies to nanoparticle (NP) labels, for use in immunoassay. We present a simple theory for the kinetics of particle capture onto a surface by means of an antibody-antigen reaction, in which the important parameter is the fraction of the particle surface that is active for reaction. We describe how increasing the generation number of the linking dendrimers significantly increased the fraction of the NP surface that is active for antigen binding and consequently also increased the assay kinetic rates. Use of dendrimers for conjugation of the NP to the antibody resulted in a significantly higher surface coverage of active antibody, in comparison with mono-valent linker chemistry. As a direct consequence, the increase in effective avidity significantly out-weighed any effect of a decreased diffusion coefficient due to the NP, when compared to that of a molecular dye-labelled antibody. The signal to noise ratio of the G4.5 dendrimer-sensitised nanoparticles out-performed the dye-labelled antibody by approximately four-fold. Particle aggregation experiments with the multi-valent antigen CRP demonstrated reaction-limited aggregation whose rate increased significantly with increasing generation number of the dendrimer linker. 相似文献