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Halina Y. Neujahr 《Applied biochemistry and biotechnology》1982,7(1-2):107-111
The enzyme phenol 2-hydroxylase was immobilized on Sepharose and used in conjunction with an O2 electrode for quantitating phenol. Similarly, catechol 1,2-oxygenase was used for quantitating catechol. A third probe was
prepared by immobilization ofTrichosporon cutaneum cells rather than purified phenol 2-hydroxylase for phenol quantitation. The whole cell system gave results comparable to
the immobilized enzyme system. 相似文献
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We have studied the hydrogenase-catalyzed production of H, when either its natural electron mediator cytochrome c3 (c3 ) or the artificial mediator methyl viologen (MV) was reduced by illumination of proflavin (PF) in the presence of ethylenediaminetetraacetate (EDTA). The reduction rates of MV and c3 were comparable at equivalent concentrations of PF, taking into account the four redox sites of c3 . However, when the concentration of c3 exceeded that of PF, the reduction rate decreased. We explain this by light quenching. In the H2 -producing system, MV was more efficient than c3 as electron mediator when the initial reaction rates were compared. However, under certain conditions with MV, the rate of H2 production decreased rapidly with time of illumination, whereas with c3 it consistently remained stable. Possible reasons for this difference are discussed.
Severe inactivation of hydrogenase was observed in the absence of the primary electron donor EDTA. It is concluded that this inactivation is caused by the excited state of PF 相似文献
Severe inactivation of hydrogenase was observed in the absence of the primary electron donor EDTA. It is concluded that this inactivation is caused by the excited state of PF 相似文献
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