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Marietjie Lutz Lize Engelbrecht Angelique Laurie Nelisa Dyayiya 《International Journal of Polymer Analysis and Characterization》2018,23(4):300-312
Efficient characterization of the distribution of antimicrobial agents within a polymer matrix is of vital importance. Two antimicrobial agents, chitin nanowhiskers (ChNWs) and chitosan-based silver nanoparticles (CTSAgNPs), were produced and incorporated separately as well as combined into a poly(ethylene-co-vinyl alcohol) (EVOH) matrix by way of electrospinning. Correlative light and electron microscopy (CLEM) and energy dispersive X-ray mapping provided a holistic approach to visualize the distribution of the combinations of AgNPs and ChNWs within EVOH fibers. CLEM analysis revealed the tendency of the ChNWs to align themselves on the surface of the fibers, while the silver nanoparticles were localized inside the polymer matrix. 相似文献
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Funda Alkan Hanife Sevgi Varlı Ph.D. Murat Demirbilek Ph.D. Engin Kaplan Ph.D. Nelisa Türkoğlu Laçin 《ChemistryOpen》2020,9(11):1181-1189
Induced pluripotent stem cells (IPSC) are preferred as an alternative source for regenerative medicine, disease modeling, and drug screening due to their unique properties. As seen from the previous studies in the literature, most of the vector systems to transfer reprogramming factors are viral-based and have some well-known limitations. This study aims to develop a non-viral vector system for the transfection of reprogramming factors. Cationic stearamide lipid nanoparticles (CSLN) were prepared via the solvent diffusion method. The obtained CSLNs were used for the delivery of plasmid DNA (pDNA) encoding Oct3/4, Sox2, Klf4, and GFP to fibroblast cell lines. The optimization studies, for zeta potential and particle size of the conjugate, was performed to achieve high cell viability. CSLN63 with 36.5±0.06 mV zeta potential and 173.6±13.91 nm size was used for the transfection of Fibroblast cells. The transfection efficiency was observed by following GFP expression and was found as 70 %±0.11. The expression of the Oct4, Sox2, Klf4 was determined by RT-qPCR; an increase was observed after the 12th cycle in Klf4 (Ct averages: 13,41), Sox2 (Ct averages; 12,4), Oct4 (Ct average; 13,77). The tendency of colonization was observed. The upregulation efficiency of Oct4 and SSEA-1 with CSLN and another non-viral vector designed for the transportation of Yamanaka factors developed in our lab previously were compared with flow cytometer analysis. 相似文献
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Nelisa Laçin Güldem Utkan Tülin Kutsal Bala Gür Dedeoğlu Işık G. Yuluğ Erhan Pişkin 《Journal of nanoparticle research》2012,14(2):694
Remodeling of the extracellular matrix resulting from increased secretion of metalloproteinase enzymes is implicated in restenosis
following balloon angioplasty. Matrix metalloproteinases (MMPs) and the tissue inhibitors of metalloproteinases play an essential
role in both normal and pathological extracellular matrix degradation. Tissue inhibitor of matrix metalloproteinase-2 is the
most extensively studied tissue inhibitor of metalloproteinases in myocardial tissue in animal models and clinical examples
of cardiac disease; therefore it is selected for this study. Gene transfer of tissue inhibitor of matrix metalloproteinase-2
may have a therapeutic potential by inhibition of matrix metalloproteinase activity. We have used PEG-lated nanoparticles
poly(St/PEG-EEM/DMAPM) which were synthesized previously in our laboratory. The nanoparticles, with an average size of 77.6 ± 2.05 nm
with a zeta potential of +64. 4 ± 1.14 mV and 201.9 ± 1.83 nm with +54.2 ± 0.77 mV were used in the transfection studies.
Zeta Potential values and size of polyplex were appropriate for an effective transfection. TIMP-2 expression was detected
by western blotting. Increased protein level in smooth muscle cells according to non-transfected smooth muscle cells confirms
the successful delivery and expression of the tissue inhibitor of matrix metalloproteinase-2 gene with the non-viral vector
transfection approach. 相似文献
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