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1.
Maya M. Zaharieva Lyudmila L. Dimitrova Stanislav Philipov Ivanka Nikolova Neli Vilhelmova Petar Grozdanov Nadya Nikolova Milena Popova Vassya Bankova Spiro M. Konstantinov Dimitrina Zheleva-Dimitrova Hristo M. Najdenski 《Molecules (Basel, Switzerland)》2022,27(1)
This study evaluated the in vitro antineoplastic and antiviral potential and in vivo toxicity of twelve extracts with different polarity obtained from the herbaceous perennial plant Geum urbanum L. (Rosaceae). In vitro cytotoxicity was determined by ISO 10993-5/2009 on bladder cancer, (T-24 and BC-3C), liver carcinoma (HEP-G2) and normal embryonic kidney (HEK-293) cell lines. The antineoplastic activity was elucidated through assays of cell clonogenicity, apoptosis induction, nuclear factor kappa B p65 (NFκB p65) activation and total glutathione levels. Neutral red uptake study was applied for antiviral activity. The most promising G. urbanum extract was analyzed by UHPLC–HRMS. The acute in vivo toxicity analysis was carried out following OEDC 423. The ethyl acetate extract of aerial parts (EtOAc-AP) exhibited the strongest antineoplastic activity on bladder cancer cell lines (IC50 = 21.33–25.28 µg/mL) by inducing apoptosis and inhibiting NFκB p65 and cell clonogenicity. EtOAc and n-butanol extracts showed moderate antiviral activity against human adenovirus type 5 and human simplex virus type I. Seventy four secondary metabolites (gallic and ellagic acid derivatives, phenolic acids, flavonoids, etc.) were identified in EtOAc-AP by UHPLC–HRMS. This extract induced no signs of acute toxicity in liver and kidney specimens of H-albino mice in doses up to 210 mg/kg. In conclusion, our study contributes substantially to the detailed pharmacological characterization of G. urbanum, thus helping the development of health-promoting phytopreparations. 相似文献
2.
Neli Koseva Piotr Kurcok Grazyna Adamus Kolio Troev Marek Kowalczuk 《Macromolecular Symposia》2007,253(1):24-32
Summary: Results on synthesis of poly(3-hydroxybutyrate)s possessing one or two hydroxyl groups at one terminus of the chain and carboxylic group at the other chain end are reported. These polymers were further functionalised via transesterification with dimethyl H-phosphonate thus incorporating a reactive/biodegradable center in the polyester backbone. Block/star-like copolymers composed of hydrophilic PEG and hydrophobic poly(3-hydroxybutyrate) segments linked by phosphoester moiety were also obtained. Chemical structure and composition of the reaction products were analysed applying different spectroscopic techniques (1H, and 31P NMR, IR and ESI-MS) and size exclusion chromatography was applied to describe molecular weight averages and distribution. 相似文献
3.
Synthesis of poly(oxyethylene phosphoramidate)s and glycopolymers via Staudinger reaction: Multivalent binding studies with Concanavalin A
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Zornica Todorova Neli Koseva Iva Ugrinova Kolio Troev 《Journal of polymer science. Part A, Polymer chemistry》2017,55(10):1730-1741
A new method for the preparation of poly(oxyethylene phosphoramidate)s and glycopolymers is developed via modification of poly(oxyethylene H‐phosphonate) which is a biodegradable, biocompatible and low toxic polymer. The phosphonate groups of the precursor are converted into tri‐coordinated phosphorus species yielding poly(oxyethylene trimethylsilyl phosphite). The latter is then reacted with different azides, including sugar azides, via Staudinger reaction to furnish the desired poly(oxyethylene phosphoramidate)s and such containing sugar moieties in the side chains attached to the P‐centers. 2002P NMR spectroscopy is applied as a powerful tool for determination of the conversion and structure of the reaction products. Studies on Concanavalin A binding to the obtained glycopolymers are performed using dynamic light scattering and analytical ultracentrifugation techniques. The viability of Human Embryonic Kidney 293 cell line is slightly affected when exposed to polyphosphoramidate glucoconjugate over a broad range of concentrations. The results obtained are encouraging for further investigations on the clustering and bio‐recognition properties of the synthesized glycopolymers. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017 , 55, 1730–1741 相似文献
4.
Electrostatic complexation of a double hydrophilic block polyelectrolyte and proteins of different molecular shape
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Maria Karayianni Radka Radeva Neli Koseva Stergios Pispas 《Journal of Polymer Science.Polymer Physics》2016,54(15):1515-1529
The electrostatic complexation between the polyelectrolyte block of the novel double hydrophilic copolymer quaternized poly(3,5‐bis(dimethylaminomethylene) hydroxystyrene)‐b‐poly(ethylene oxide) (QNPHOS‐PEO) and proteins of different molecular shape, that is globular bovine serum albumin (BSA) or rod‐like bovine fibrinogen (FBG), is investigated by means of dynamic, static, and electrophoretic light scattering, as well as analytical ultracentrifugation measurements. The solution behavior, structure, and properties of the formed complexes at pH 7 and 0.01 M ionic strength, as a function of the protein concentration in the solution (or equivalently the charge ratio of the two components), depend on the protein concentration and molecular characteristics. Moreover, the structure of the complexes is greatly influenced by the intrinsic structure of the block polyelectrolyte, which forms rather loose multichain aggregates, due to hydrophobic interactions. A direct correlation between the stability of the preformed complexes against the increase of the solution ionic strength and their structure is established. Finally, the spectroscopic structural investigation of both complexed proteins reveals no signs of protein denaturation upon complexation. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2016 , 54, 1515–1529 相似文献
5.
N.?I.?StoilovaEmail author J.?Van?der?Jeugt 《International Journal of Theoretical Physics》2005,44(8):1157-1165
The Lie superalgebra su(1|5) has irreducible representations of dimension 32, in which the 32 fundamental fermions of one generation (leptons and
quarks, of left and right chirality, and their antiparticles) can be accommodated. The branching of these su(1|5) representations with respect to its subalgebra su(3)× su(2)× u(1) reproduces precisely the classification of these fundamental fermions according to the gauge group su(3)c× su(2)w× u(1)w of the Standard Model. Furthermore, a simple construction of the relevant representations is given, and some consequences
are discussed. 相似文献
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7.
Despite their diverse origin, networks of large real-world systems reveal a number of common properties including small-world phenomena, scale-free degree distributions and modularity. Recently, network self-similarity as a natural outcome of the evolution of real-world systems has also attracted much attention within the physics literature. Here we investigate the scaling of density in complex networks under two classical box-covering renormalizations–network coarse-graining–and also different community-based renormalizations. The analysis on over 50 real-world networks reveals a power-law scaling of network density and size under adequate renormalization technique, yet irrespective of network type and origin. The results thus advance a recent discovery of a universal scaling of density among different real-world networks [P.J. Laurienti, K.E. Joyce, Q.K. Telesford, J.H. Burdette, S. Hayasaka, Universal fractal scaling of self-organized networks, Physica A 390 (20) (2011) 3608–3613] and imply an existence of a scale-free density also within–among different self-similar scales of–complex real-world networks. The latter further improves the comprehension of self-similar structure in large real-world networks with several possible applications. 相似文献
8.
Margarita Popova gnes Szegedi Manuela Oykova Hristina Lazarova Neli Koseva Magdolna R. Mihlyi Pavletta Shestakova 《Molecules (Basel, Switzerland)》2021,26(12)
Mono- and bimetallic Ni-, Ru- and Pt-modified hierarchical ZSM-5 materials were prepared by impregnation technique and characterized by X-ray diffraction (XRD), N2 physisorption, temperature-programmed reduction (TPR–TGA), ATR–FTIR and solid state NMR spectroscopy. Formation of finely dispersed nickel, ruthenium and platinum species was observed on the bimetallic catalysts. It was found that the peculiarity of the used zeolite structure and the modification procedure determine the type of formed metal oxides and their dispersion and reducibility. The samples’ acidity was studied via FTIR spectroscopy of adsorbed pyridine. The changes in the zeolite structure were studied via solid-state NMR spectroscopy. The catalysts were investigated in a gas-phase hydrodeoxygenation, transalkylation and dealkylation reaction of model lignin derivative molecules for phenol production. 相似文献
9.
Neli Mintcheva Ivelina Georgieva Tzvetan Mihaylov Natasha Trendafilova Makoto Tanabe Kohtaro Osakada 《Journal of organometallic chemistry》2012,697(1):23-32
The molecular structure and spectroscopic properties of a series of phenylplatinum complexes containing silsesquioxanate and phosphine ligands with general formula trans-[Pt{O10Si7(R)7(OH)2}(Ph)(L)2] (1: R = cyclo-C5H9, L = PEt3; 2: R = iso-C4H9, L = PEt3; 3: R = CH3, L = PEt3; 4: R = cyclo-C5H9, L = PMe3; 5: R = cyclo-C5H9, L = PMe2Ph; 6: R = cyclo-C5H9, L = PPh2Me; 7: R = cyclo-C5H9, L = PPh3) have been investigated by DFT/OPW91/6-31G(d) calculations, 1H, 13C, 29Si and 31P NMR and IR spectroscopy. DFT molecular modeling based on available X-ray and NMR data for complexes 1 and 2 allowed deriving structure-NMR spectra correlations. It was found that the alkyl substituents (R) attached to Si atoms, cyclo-C5H9, iso-C4H9 and CH3, slightly influence the geometry and multinuclear NMR parameters of the complexes in the series studied. The molecular structures of the Pt(II) complexes with R = cyclo-C5H9 (4–7) were predicted by DFT calculations of their simplified models with R = CH3 (4′?7′). The geometry optimizations of 4′?7′ showed square-planar configuration of Pt(II) center bonded to two trans phosphine ligands, a phenyl group and an O-monocoordinated silsesquioxanate. The structures 4′?6′ are stabilized by two intramolecular hydrogen bonds similar to 1 and 2. A fast conformer exchange process A?B and switching of H-bonds in solution of 1–6 were suggested based on (i) the calculated conformer energies and small barrier of the process, and (ii) the observed single 1H NMR signal at low magnetic field. The stability of the Pt(II) complexes depends on the nature of the phosphine ligands and decreases in the order PMe2Ph > PMe3 > PPh2Me > PEt3 > PPh3. The PPh3 ligands attached to Pt(II) in 7 cause the largest geometry changes and a new set of weaker hydrogen bonds. The comparison of the calculated NMR and IR parameters with the experimental spectroscopic data reveals good coincidence and thus confirmed the suggested molecular structures. 相似文献
10.
Brum RL Cardoso CA Honda NK Andreu MP Viana LL 《Chemical & pharmaceutical bulletin》2006,54(5):754-757
A method for sample preparation and analysis by high performance liquid chromatography with UV detection (HPLC-UV) was developed for analysis of jatrophone in "cacha?a" prepared with Jatropha elliptica, administered orally, employed in Brazil for the treatment of venomous snake bites. The linearity, accuracy, precision of the procedure was evaluated. Analytical curve for jatrophone was linear in the range of 16.24-81.20 microg ml(-1). The recovery of the jatrophone in the samples analyzed was 98.99-99.89%. The percent coefficient of variation for the quantitative analysis of the "cacha?a" in the analyses was under 2%. 相似文献