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1.
Lee BS Krishnanchettiar S Lateef SS Lateef NS Gupta S 《Rapid communications in mass spectrometry : RCM》2005,19(18):2629-2635
A method, which utilizes microwave-assisted partial acid hydrolysis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS), to elucidate oligosaccharide composition of intact glycoproteins is presented here. Glycoproteins, such as ribonuclease B, avidin, alpha1-acid glycoprotein, and fetuin, are used as model systems to demonstrate this technique. Partial cleavage of oligosaccharides from whole intact glycoproteins with trifluoroacetic acid was observed after a short exposure to microwaves. Due to the high-resolution mass spectra obtained by MALDI-TOFMS from glycoproteins with molecular weights less than 20 kDa, the compositions of oligosaccharides are readily derived for ribonuclease B and avidin. The data agree with the proposed oligosaccharide structures of ribonuclease B (five glycoforms) and avidin (eight glycoforms). Larger glycoproteins such as alpha1-acid glycoprotein (many glycoforms) and fetuin (many glycoforms) exhibited only broad peaks with no glycoform resolution. Nevertheless, this method can be used successfully for analysis of glycoproteins with molecular weights greater than 20 kDa to determine the presence or absence of glycosylation. 相似文献
2.
Bao‐Shiang Lee Sangeeth Krishnanchettiar Syed Salman Lateef Nabila Salman Lateef Shalini Gupta 《中国化学会会志》2006,53(3):745-750
Avidin functional affinity electrophoresis (AFAEP) is substituted for an avidin affinity column (AAC) to capture biotinylated peptides in the Isotope‐Coded Affinity Tagging (ICAT) technique which is a valuable tool in quantitative proteomics. In this new technique, the AFAEP‐captured ICAT‐labeled biotinylated peptides are extracted with the biotin tag intact from the polyacrylamide gel piece with aqueous 95% formamide (pH 8.2) at 65 °C for 20 min, and then detected by a matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometer. Bovine serum albumin (BSA) and the 12C‐ and 13C‐ICAT reagents are used to test this AFAEP‐ICAT technique. The results show that both AFAEP and AAC methods provide quantitative information of the relative amounts of 12C‐ and 13C‐ICAT‐labeled biotinylated tryptic peptides of BSA in a sample. Compared with AAC, the AFAEP is cheaper to perform, more stringent in capturing the biotinylated peptides, and capable of simultaneously processing multiple samples. 相似文献
3.
Kherroubi Seddik Benkahla Youb Khaled Labsi Nabila Ragui Karim Bensaci Abdelghani Boutra Abdelkader Ouyahia Seif-Eddine Benzema Mahdi 《Journal of Thermal Analysis and Calorimetry》2021,144(3):623-646
Journal of Thermal Analysis and Calorimetry - Through this paper, three-dimensional fluid flow and heat transfer of Al2O3 nanofluid within ventilated enclosures was taken into consideration.... 相似文献
4.
Reda M. Fikry Nabila A. Ismael Adel A. El-Bahnasawy Aymen A. Sayed El-Ahl 《Phosphorus, sulfur, and silicon and the related elements》2013,188(6):1227-1236
Several new 2-arylidinoamino-4-(3-coumaryl)thiazole derivatives were synthesized via the reaction of 2-amino-4-(coumaryl)thiazole with aromatic aldehydes and other reagents. Structural elucidations were based on elementary analysis and spectral data studies. 相似文献
5.
Nabila A. Al-Jaber Amal S.A. Bougasim Makarem M.S. Karah 《Journal of Saudi Chemical Society》2012,16(1):45-53
Michael addition reaction of 1,3-diphenyl-propenone 1a, e, and f with o-amino thiophenol in the presence of indium trichloride gave the benzothiazine derivatives 2a–c. Condensation of the compound 1a, e with o-phenylene diamine in triethylamine gave the benzodiazepine derivatives 3a–b. Cyclization of 1d with malononitrile in the presence of NaOR/EtOH gave the compound 4. Addition of thiobarbituric acid in triethylamin to 1a gave 5. Condensation of compound 1c with malononitrile in the presence ammonium acetate gave compound 6. 1,3-diphenyl-propenone 1a used as key starting chalcone to react with different active methylene reagents under phase-transfer catalysis condition gave compound 7–9. The structures of the prepared compounds were mainly confirmed on the basis of spectroscopic methods. 相似文献
6.
In this paper, we exhibit the colorizing of brass surfaces by forming femtosecond laser induced microstructures on the sample surfaces. A variety of single colors such as brown, yellow, green, and black are introduced on brass surfaces by engineering periodic microgratings, microholes, and ring-shaped micro-patterns using Single Beam Direct Laser Writing (SBDLW) technique. The color of the micro-structured brass surfaces is certainly dependent on the width, depth, and period of the microstructures. Finally, we explain, in brief, the colorizing mechanism of metals by femtosecond laser induced microstructures. 相似文献
7.
Nabila M. Ibrahim 《Phosphorus, sulfur, and silicon and the related elements》2013,188(8):1773-1784
The behavior of 4-hydroxycoumarin (1), 7-hydroxycoumarin (2), 4-hydroxy-bergapten (3), and 4-hydroxyisopimpinellin (4) toward sulfur reagents, namely, thionyl chloride, phosphorus pentasulfide, thiolacetic acid, and Lawesson's reagent (5), was studied. The nature of products in each case depended upon the type of reactants and reaction conditions. Possible reaction mechanisms were considered, and structural elucidations of the new products were based upon compatible elementary and spectroscopic evidences. 相似文献
8.
9.
[structure: see text] The synthesis of an advanced component leading to (-)-kendomycin is described. The synthetic scheme features the application of asymmetric conjugate addition methodology for the early generation of the C13-C14 (E)-trisubstituted olefin, providing an efficient assembly of the ansa chain. Condensation reactions probe two strategies for attachment of the aromatic system. 相似文献
10.
Mohamad Sh. Ramadan Nabila M. El-Mallah Sherif M. Abd-Elmenem 《Journal of Dispersion Science and Technology》2013,34(8):1110-1120
AbstractThe interaction between anionic dyes [Reactive Orange 122 (R.O 122), Reactive Blue 19 (R.B 19), Reactive Violet 5 (R.V 5) and Acid Green 20 (A.G 20)] with cationic surfactant cetyltrimethylammoniun bromide (CTAB) has been investigated by spectrophotometry and conductance technique. The used dyes are characterized by tautomeric behavior which affects the mechanism of the interaction. Various parameters such as dye structure, surfactant composition, solvent composition, temperature and pH of the medium were studied. The spectral data were applied for calculating the binding constant between dye and surfactant (Kb), fraction of micellization (?mic), and standard free energy change of binding (ΔG°b) in 0,10,20 and 30 v/v % acetonitile (AN). Conductance technique was constructed to estimate the ion pairing constant (Ka) at different temperatures and v/v % AN. Thermodynamic parameters (ΔG°, ΔH° and ΔS°) for ion pair formation were evaluated. The role of hydrophobic and electrostatic effect on dye-surfactant interaction was discussed. 相似文献