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The covalent conjugates of cellulase from Aspergillus niger were prepared with various molar ratios by using dextran. The conjugate (nE/nD: 1/5) showed higher activity than purified enzyme at all temperatures after 1 h of incubation and its activity could also be measured at higher temperature. Also, this conjugate lost only 60% activity in 2 h at 70°C in comparison to the purified enzyme, which lost all its activity. In addition, conjugation protected cellulase against denaturation in the presence of sodium dodecylsulfate (residual activity of about 80%) and inactivation by air bubbles (residual activity of about 50% for 4 h). 相似文献
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Melda Altikatoglu Candan Arioz Yeliz Basaran Huriye Kuzu 《Central European Journal of Chemistry》2009,7(3):423-428
Stabilization of Horseradish Peroxidase (HRP; EC 1.11.1.7) against temperature and pH via the formation of the conjugates
obtained by multipoint covalent bonding of dextran aldehyde (DA) to the enzyme were studied. Hence, three different molar
weighted dextrans (17.5 kD, 75 kD, 188 kD) were covalently bonded to purified enzyme with different molar ratios (nHRP/nDA 20/1, 10/1, 1/1, 1/5, 1/10, 1/15, 1/20). The thermal stabilities of the obtained conjugates were evaluated with the activities
determined at different temperatures (25, 30, 35, 40, 50, 60, 70, 80°C) applying 60 minutes incubation time. Conjugates formed
were characterized by gel-permeation chromatography (GPC) and fluorescence techniques. The conjugate synthesized using dextran
75 kDa with nHRP/nDA 1/10 molar ratio showed better thermal stability than other conjugates and purified enzyme at pH 7. This conjugate also has
wider activity pH range than purified enzyme. In addition, mentioned conjugate at pH 7 had very long storage lifetime compared
to purified enzyme at +4°C and room temperature; which is considered a favorable feature for usage in practice.
相似文献
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Kani Zilbeyaz Hamdullah Kilic Melda Sisecioglu Hasan Ozdemir Azize Alayli Güngör 《Tetrahedron: Asymmetry》2012,23(8):594-601
The kinetic resolution of a variety of secondary para-substituted phenylethyl hydroperoxides by Raphanus sativus L. (black radish peroxidase) in the presence of guaiacol is reported. The peroxidase enzyme recognized (R)-configured alkyl aryl hydroperoxides, which furnished optically active (S)-hydroperoxides and (R)-alcohols. Kinetic resolution of tertiary hydroperoxides by the enzyme was unsuccessful. This study also shows how the optically active p-substituted (S)-hydroperoxides obtained can be employed as enantioselective oxidants in the asymmetric Weitz–Scheffer epoxidation of E-chalcone in the presence of KF-Al2O3 as a base. In all cases, a chalcone epoxide with the (αS,βR)-configuration was obtained as the major isomer. Under the optimized reaction conditions, the enantiomeric excess of the chalcone epoxide was obtained in up to 49% in CH3CN at ?40 °C. 相似文献
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This paper presents a Hilbert transform-based approach to analyze vocal fold vibrations in human subjects exhibiting normal and abnormal voice productions. This new approach is applied to the analysis of glottal area waveform (GAW) and is capable of providing useful information on the vocal fold vibration. The GAW is extracted from high-speed laryngeal images by delineating the glottal edge for each image frame. An analytic signal is generated through the Hilbert transform of the GAW, which yields a recognizable pattern of the vocal fold vibration in the analytic phase plane. The vibratory pattern is comprehensive and can be correlated with specific voice conditions. Quantitative measures of the glottal perturbation are introduced using the analytic amplitude and instantaneous frequency obtained from the analysis. Examples of clinical voice recordings are used to evaluate and test the effectiveness of this approach in providing qualitative representation and quantitative characteristics of vocal fold vibratory behavior. The results demonstrate the potential of using this new analytical tool incorporated with the high-speed laryngeal imaging modality for clinical voice assessment. 相似文献
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Selva Onder Mithat Celebi Melda Altikatoglu Arzu Hatipoglu Huriye Kuzu 《Applied biochemistry and biotechnology》2011,163(3):433-443
This study evaluates the potential of the enzyme horseradish peroxidase in the decolorization of one common industrial azo
dye, naphthol blue black. Studies are carried out to understand the process parameters such as pH, temperature and reaction
time. The enzymatic decolorization of the dye was examined by UV-Vis spectrophotometer and LC-MS measurements. Temperature
and pH conditions were optimized for obtaining high azo-dye decolorization. Azo-dye removal at a pH range 4-6 was found to
be the highest for all temperatures. After 5 minutes of treatment, the color removal of dye was ca. 80-90%. The LC-MS and
spectrophotometric analyses indicated that the decolorization of the azo dye with enzyme was due to the reduction of the azo
bonds. This study verifies the viability of the use of the horseradish peroxidase in the decolorization of naphthol blue black. 相似文献
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Melda Altikatoglu Yeliz Basaran Candan Arioz Ayse Ogan Huriye Kuzu 《Applied biochemistry and biotechnology》2010,160(8):2187-2197
Multipoint covalent bonding of glucose oxidase (EC 1.1.3.4) to hydrophilic natural polymer dextran and optimization of procedures
to obtain, with enhanced temperature and pH stabilities, were studied. Purified enzyme was conjugated with various molecular
weight dextrans (17.5, 75, and188 kD) in a ratio of 20:1, 10:1, 1:1, 1:5, 1:10, 1:15, and 1:20. After 1 h of incubation at
pH 7, the activities of purified enzyme and conjugates were determined at different temperatures (25°C, 30°C, 35°C, 40°C,
50°C, 60°C, 70°C, and 80°C), and the results were evaluated for thermal resistance. Increases in temperature from 25°C to
50°C did not change the activities of the conjugates. The conjugate, which was prepared with 75 kDa dextran in a molar ratio
of 1:5, showed the highest thermal resistance and even the activity still remains at 80°C at pH 7.0. This conjugate also displayed
activity in a wide pH range (pH 4.0–7.0) at high temperatures. Conjugate, which was synthesized with 75 kDa dextran in a molar
ratio of 1:5, appears to be feasible and useful for biotechnological applications. 相似文献