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JPC – Journal of Planar Chromatography – Modern TLC - The Ziziphus fruits are recognized as a rich source of biologically active compounds, such as polysaccharides, phenolics,... 相似文献
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Mohini Ghatge Yogesh Mawal Sushama Gaikwad Vasanti Deshpande 《Applied biochemistry and biotechnology》1991,31(1):11-20
A procedure was developed to purify glucose/xylose isomerase from cell extract of Streptomyces sp. NCIM 2730 using immunoaffinity chromatography. High-titer polyclonal antibodies were raised in rabbit using electrophoretically homogeneous glucose/xylose isomerase as an antigen. The specificity of antibodies was confirmed by double immunodiffusion, rocket electrophoresis, and Western-blot ELISA, which revealed the presence of a single immunoreactive protein with an Mr of 40,000. The antibodies recognized 2-3 antigenic determinants/mol of enzyme and were found to partially neutralize the enzymatic activity in an immunotitration experiment. The affinity gel was prepared by coupling antibodies at pH 10.0 to divinyl sulfone-activated Sepharose CL-4B. The glucose/xylose isomerase purified by immunoaffinity chromatography yielded 75% recovery with a single enzymatically active protein band on gel electrophoresis and showed specific activity of 16 U/mg. The crossreaction of the antibodies with glucose isomerase from other actinomycetes indicated that they share common epitopes. 相似文献
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Kapoor Manish Gupta Raghbir Chand Mawal Pooja Pradhan Saroj Kumar 《平面色谱法杂志一现代薄层色谱法》2019,32(2):109-114
JPC – Journal of Planar Chromatography – Modern TLC - This study is a first attempt to present a report about a high-performance thin-layer chromatography (HPTLC)—densitometric... 相似文献
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