排序方式: 共有13条查询结果,搜索用时 15 毫秒
1.
2.
Trevitt CR Craven CJ Milanesi L Syson K Mattinen ML Perkins J Annila A Hunter CA Waltho JP 《Chemistry & biology》2005,12(1):89-97
Using calmodulin antagonism as a model, it is demonstrated that, under circumstances in which binding sites are motionally independent, it is possible to create bifunctional ligands that bind with significant affinity enhancement over their monofunctional counterparts. Suitable head groups were identified by using a semiquantitative screen of monofunctional tryptophan analogs. Two bifunctional ligands, which contained two copies of the highest-affinity head group tethered by rigid linkers, were synthesized. The bifunctional ligands bound to calmodulin with a stoichiometry of 1:1 and with an affinity enhancement over their monofunctional counterparts; the latter bound with a stoichiometry of 2:1 ligand:protein. A lower limit to the effective concentrations of the domains of calmodulin relative to each other (0.2-2 mM) was determined. A comparable effective concentration was achieved for bifunctional ligands based on higher-affinity naphthalene sulphonamide derivatives. 相似文献
3.
Reinhard Meusinger Christian Duschek Erich Kleinpeter Rolf Borsdorf Kalevi Pihlaja Jorma Mattinen 《Monatshefte für Chemie / Chemical Monthly》1988,119(8-9):1019-1025
2,6-Dichloro-4-methoxy-heptanes may exist as 2 optical active isomers and 2 meso compounds. By double addition of ,-dichloro-dimethylether to propene a mixture of these stereoisomers was obtained. They were separated by HPLC. Using1H- and13C-NMR-spectroscopy their configuration could be established. 相似文献
4.
Tyramine, histamine, putrescine and cadaverine, the most common biogenic amines indicating the food quality, were studied in the transglutaminase-catalyzed reaction. Transglutaminase (protein-glutamine gamma-glutamyltransferase EC 2.3.2.13) catalyzes an acyl transfer reaction between a donor substrate and an acceptor substrate (e.g. biogenic amine) and forms a cross-linkage between substrates with a release of ammonia. The reaction can be monitored by measuring the ammonia produced in the reaction. The concentration of produced ammonia was found to be proportional to the concentration of biogenic amine and could hence be used to determination of biogenic amines in food matrixes. 相似文献
5.
6.
Dimitrije Cicmil Salzitsa Anastasova Andrew Kavanagh Dermot Diamond Ulriika Mattinen Johan Bobacka Andrzej Lewenstam Aleksandar Radu 《Electroanalysis》2011,23(8):1881-1890
In this paper, we describe a new type of polymer membrane‐based reference electrode (RE) based on ionic liquids (ILs), in both liquid‐contact (LCRE) and solid‐contact reference electrode (SCRE) forms. The ILs used were bis(trifluoromethane sulfonyl)amid with 1‐alkyl‐3‐methyl‐imidazolium as well as phosphonium and ammonium cations. In addition to their charge stabilisation role, it was found that the ILs also functioned as effective plasticizers in the PVC matrix. The LCREs and SCREs were prepared using the same design as their corresponding indicator electrodes. LCREs were prepared by casting in glass rings while SCREs were prepared on platforms made using screen‐printing technology, with poly(3‐octylthiophene‐2,5 diyl) (POT) as the intermediate polymer. After potentiometric characterization of the response mechanism, the practical performance of the REs was studied using potentiometric titrations (Pb2+ and pH), and characterised using cyclic voltammetry and impedance spectroscopy. All results were compared via parallel experiments in which the novel RE was substituted by a conventional double junction Ag/AgCl reference electrode. The mechanism of response is most likely based on a limited degree of partitioning of IL ions into the sample thereby defining aquo‐membrane interfacial potential. Despite their simple nature and construction, the REs showed excellent signal stability, and performed well in the analytical experiments. The identical mode of fabrication to that of the equivalent indicator (or Ion‐Selective Electrode, ISE) will facilitate mass‐production of both indicator and reference electrode using the same fabrication line, the only difference being the final capping membrane composition. 相似文献
7.
Nitrogen quantum yields are reported for the photolysis of C2F5N?NC2F5 at 3660 Å over the pressure range 2–10 cm from 25° to 150°c. The Stern–Volmer plots obtained are discussed and compared with those obtained with azoethane and azoisopropane. 相似文献
8.
Miika Mattinen Peter J. King Leonid Khriachtchev Mikko J. Heikkilä Ben Fleming Simon Rushworth Kenichiro Mizohata Kristoffer Meinander Jyrki Räisänen Mikko Ritala Markku Leskelä 《Materials Today Chemistry》2018
Molybdenum forms a range of oxides with different stoichiometries and crystal structures, which lead to different properties and performance in diverse applications. Herein, crystalline molybdenum oxide thin films with controlled phase composition are deposited by atomic layer deposition. The MoO2(thd)2 and O3 as precursors enable well-controlled growth of uniform and conformal films at 200–275 °C. The as-deposited films are rough and, in most cases, consist of a mixture of α- and β-MoO3 as well as an unidentified suboxide MoOx (2.75 ≤ x ≤ 2.89) phase. The phase composition can be tuned by changing deposition conditions. The film stoichiometry is close to MoO3 and the films are relatively pure, the main impurity being hydrogen (2–7 at-%), with ≤1 at-% of carbon and nitrogen. Post-deposition annealing is studied in situ by high-temperature X-ray diffraction in air, O2, N2, and forming gas (10% H2/90% N2) atmospheres. Phase-pure films of MoO2 and α-MoO3 are obtained by annealing at 450 °C in forming gas and O2, respectively. The ability to tailor the phase composition of MoOx films deposited by scalable atomic layer deposition method represents an important step towards various applications of molybdenum oxides. 相似文献
9.
P. Lukkari J. Hannuksela M.-L. Mattinen M. Virolainen Vera M. A. Hkkinen M.-L. Riekkola 《Journal of separation science》1990,13(3):170-172
The on-line coupled LC-GC technique was applied to the analysis of several metal chelates of N,N-diethyldithiocarbamic acid. A 10-port valve interface was used to couple the LC and GC instruments. Conditions during sample transfer into the GC gave fully concurrent solvent evaporation. The chelates investigated were separated with a short apolar fused silica column. LC preseparation was made with cyano or amino phases using a hexane/dichloromethane mixture as eluent. On-line LC-GC combination seems to be very suitable for the separation of the metal chelates studied. 相似文献
10.
Outi Nivala Greta Faccio Mikko Arvas Perttu Permi Johanna Buchert Kristiina Kruus Maija-Liisa Mattinen 《BMC biochemistry》2017,18(1):15