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Dr. Ulrike Rieder Prof. Dr. Nathan W. Luedtke 《Angewandte Chemie (International ed. in English)》2014,53(35):9168-9172
5‐Vinyl‐2′‐deoxyuridine (VdU) is the first reported metabolic probe for cellular DNA synthesis that can be visualized by using an inverse electron demand Diels–Alder reaction with a fluorescent tetrazine. VdU is incorporated by endogenous enzymes into the genomes of replicating cells, where it exhibits reduced genotoxicity compared to 5‐ethynyl‐2′‐deoxyuridine (EdU). The VdU–tetrazine ligation reaction is rapid (k≈0.02 M ?1 s?1) and chemically orthogonal to the alkyne–azide “click” reaction of EdU‐modified DNA. Alkene–tetrazine ligation reactions provide the first alternative to azide–alkyne click reactions for the bioorthogonal chemical labeling of nucleic acids in cells and facilitate time‐resolved, multicolor labeling of DNA synthesis. 相似文献
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Juliana S Luz Celso RR Ramos Márcia CT Santos Patricia P Coltri Fernando L Palhano Debora Foguel Nilson IT Zanchin Carla C Oliveira 《BMC biochemistry》2010,11(1):22
Background
The archaeal exosome is formed by a hexameric RNase PH ring and three RNA binding subunits and has been shown to bind and degrade RNA in vitro. Despite extensive studies on the eukaryotic exosome and on the proteins interacting with this complex, little information is yet available on the identification and function of archaeal exosome regulatory factors. 相似文献6.
We present two linearization-based algorithms for mixed-integer nonlinear programs (MINLPs) having a convex continuous relaxation. The key feature of these algorithms is that, in contrast to most existing linearization-based algorithms for convex MINLPs, they do not require the continuous relaxation to be defined by convex nonlinear functions. For example, these algorithms can solve to global optimality MINLPs with constraints defined by quasiconvex functions. The first algorithm is a slightly modified version of the LP/NLP-based branch-and-bouund \((\text{ LP/NLP-BB })\) algorithm of Quesada and Grossmann, and is closely related to an algorithm recently proposed by Bonami et al. (Math Program 119:331–352, 2009). The second algorithm is a hybrid between this algorithm and nonlinear programming based branch-and-bound. Computational experiments indicate that the modified LP/NLP-BB method has comparable performance to LP/NLP-BB on instances defined by convex functions. Thus, this algorithm has the potential to solve a wider class of MINLP instances without sacrificing performance. 相似文献
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Ren J Qin H Wang J Luedtke NW Wang E Wang J 《Analytical and bioanalytical chemistry》2011,399(8):2763-2770
In this study we have used two fluorescent probes, tetrakis(diisopropylguanidino)-zinc-phthalocyanine (Zn-DIGP) and N-methylmesoporphyrin IX (NMM), to monitor the reassembly of “split” G-quadruplex probes on hybridization with an arbitrary
“target” DNA. According to this approach, each split probe is designed to contain half of a G-quadruplex-forming sequence
fused to a variable sequence that is complementary to the target DNA. Upon mixing the individual components, both base-pairing
interactions and G-quadruplex fragment reassembly result in a duplex–quadruplex three-way junction that can bind to fluorescent
dyes in a G-quadruplex-specific way. The overall fluorescence intensities of the resulting complexes were dependent on the
formation of proper base-pairing interactions in the duplex regions, and on the exact identity of the fluorescent probe. Compared
with samples lacking any “target” DNA, the fluorescence intensities of Zn-DIGP-containing samples were lower, and the fluorescence
intensities of NMM-containing samples were higher on addition of the target DNA. The resulting biosensors based on Zn-DIGP
are therefore termed “turn-off” whereas the biosensors containing NMM are defined as “turn-on”. Both of these biosensors can
detect target DNAs with a limit of detection in the nanomolar range, and can discriminate mismatched from perfectly matched
target DNAs. In contrast with previous biosensors based on the peroxidase activity of heme-bound split G-quadruplex probes,
the use of fluorescent dyes eliminates the need for unstable sensing components (H2O2, hemin, and ABTS). Our approach is direct, easy to conduct, and fully compatible with the detection of specific DNA sequences
in biological fluids. Having two different types of probe was highly valuable in the context of applied studies, because Zn-DIGP
was found to be compatible with samples containing both serum and urine whereas NMM was compatible with urine, but not with
serum-containing samples. 相似文献
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Renata Monteiro-Maia Maria B Ortigão-de-Sampaio Rosa T Pinho Luiz RR Castello-Branco 《Journal of immune based therapies and vaccines》2006,4(1):1-6
We hypothesize that the energy strategy of a cell is a key factor for determining how, or if, the immune system interacts
with that cell. Cells have a limited number of metabolic states, in part, depending on the type of fuels the cell consumes.
Cellular fuels include glucose (carbohydrates), lipids (fats), and proteins. We propose that the cell's ability to switch
to, and efficiently use, fat for fuel confers immune privilege. Additionally, because uncoupling proteins are involved in
the fat burning process and reportedly in protection from free radicals, we hypothesize that uncoupling proteins play an important
role in immune privilege. Thus, changes in metabolism (caused by oxidative stresses, fuel availability, age, hormones, radiation,
or drugs) will dictate and initiate changes in immune recognition and in the nature of the immune response. This has profound
implications for controlling the symptoms of autoimmune diseases, for preventing graft rejection, and for targeting tumor
cells for destruction. 相似文献
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Dynamical interactions between a scanning tip and a silicon substrate are investigated using molecular dynamics simulations of both the constant-height and constant-force scan modes. Localized temporary and permanent modifications of the substrate occur, depending on tip-substrate-strate separation and scan geometry. Implications for resolving structural and force characteristics in scanning tip spectroscopies, employing atomically sharp as well as large ordered of disordered tips are discussed. 相似文献