Evaluation of 3D Printed Gelatin‐Based Scaffolds with Varying Pore Size for MSC‐Based Adipose Tissue Engineering

Adipose tissue engineering aims to provide solutions to patients who require tissue reconstruction following mastectomies or other soft tissue trauma. Mesenchymal stromal cells (MSCs) robustly differentiate into the adipogenic lineage and are attractive candidates for adipose tissue engineering. This work investigates whether pore size modulates adipogenic differentiation of MSCs toward identifying optimal scaffold pore size and whether pore size modulates spatial infiltration of adipogenically differentiated cells. To assess this, extrusion‐based 3D printing is used to fabricate photo‐crosslinkable gelatin‐based scaffolds with pore sizes in the range of 200–600 µm. The adipogenic differentiation of MSCs seeded onto these scaffolds is evaluated and robust lipid droplet formation is observed across all scaffold groups as early as after day 6 of culture. Expression of adipogenic genes on scaffolds increases significantly over time, compared to TCP controls. Furthermore, it is found that the spatial distribution of cells is dependent on the scaffold pore size, with larger pores leading to a more uniform spatial distribution of adipogenically differentiated cells. Overall, these data provide first insights into the role of scaffold pore size on MSC‐based adipogenic differentiation and contribute toward the rational design of biomaterials for adipose tissue engineering in 3D volumetric spaces.     

A dialogue on the use of arithmetic in geometry: Van Ceulen’s and Snellius’s Fundamenta Arithmetica et Geometrica

Snellius’s Fundamenta Arithmetica et Geometrica (1615) is much more than a Latin translation of Ludolph van Ceulen’s Arithmetische en Geometrische Fondamenten. Willebrord Snellius both adapted and commented on the Dutch original in his Fundamenta, and thus his Latin version can be read as a dialogue between representatives of two different approaches to mathematics in the early modern period: Snellius’s humanist approach and Van Ceulen’s practitioner’s approach. This article considers the relationship between the Dutch and Latin versions of the text and, in particular, puts some of their statements on the use of numbers in geometry under the microscope. In addition, Snellius’s use of the Fundamenta as an instrument to further his career is explained.     

Analysis of several subcycling schemes in partitioned simulations of a strongly coupled fluid-structure interaction

Fluid-structure interaction (FSI) simulations are used extensively to calculate the vibration of structures subjected to an internal or external flow. In the case of partitioned FSI simulations, separate flow and structure solvers are used, which requires some kind of coupling between both. The time step in both solvers is typically taken the same, but this unnecessarily leads to long calculation times when the time step is small due to stability reasons in one of the two solvers. Subcycling, the procedure where the time step of one solver is chosen smaller than the time step used in the other solver, may reduce the computational cost of the FSI simulation. The subcycling procedure can be either explicit or implicit, the latter implying the use of coupling iterations in each time step. Contrary to explicit subcycling, no stability analyses of implicit subcycling schemes are found in the literature. In this paper, the temporal stability of the implicit subcycling procedure is investigated. The one-dimensional flow in an elastic cylindrical tube is studied analytically. The results of this analysis are subsequently compared to a partitioned two-dimensional axisymmetric FSI calculation with implicit coupling between the flow and structure solvers.     

Engineered 3D microporous gelatin scaffolds to study cell migration

Here we present a facile method to fabricate microporous hydrogel scaffolds that can be functionalized with a chemokine gradient. These scaffolds allow studying cellular responses in a 3D environment.     

Polymeric Multilayer Capsules in Drug Delivery

Recent advances in medicine and biotechnology have prompted the need to develop nanoengineered delivery systems that can encapsulate a wide variety of novel therapeutics such as proteins, chemotherapeutics, and nucleic acids. Moreover, these delivery systems should be “intelligent”, such that they can deliver their payload at a well‐defined time, place, or after a specific stimulus. Polymeric multilayer capsules, made by layer‐by‐layer (LbL) coating of a sacrificial template followed by dissolution of the template, allow the design of microcapsules in aqueous conditions by using simple building blocks and assembly procedures, and provide a previously unmet control over the functionality of the microcapsules. Polymeric multilayer capsules have recently received increased interest from the life science community, and many interesting systems have appeared in the literature with biodegradable components and biospecific functionalities. In this Review we give an overview of the recent breakthroughs in their application for drug delivery.     

Exclusive meson production at HERMES

Hard exclusive production of mesons in deep-inelastic scattering allows one to probe the so-far unknown Generalized Parton Distributions (GPDs) of the nucleon. The HERMES experiment has measured several different observables in exclusive meson production by scattering the 27.6GeV HERA lepton beam off an internal fixed gaseous target. Recent results on exclusive π ⁺, ρ ⁰ and pion pair production will be presented.