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A preliminary study of using maleic anhydride copolymer for protein binding has been carried out.The polymeric films were prepared by compression of the purified resin and annealing the film to induce efficient back formation of the anhydride groups.The properties of the film surface were analyzed by attenuated total reflection Fourier transforms infrared spectroscopy and water contact angle measurements.The protein content was determined by Bradford assay.To obtain optimum conditions,immersion time for protein binding was examined.Results revealed that proteins can be successfully immobilized onto the film surface via covalent linkage.The efficiency of the covalent binding of the extractable protein to maleic anhydride-polyethylene film was estimated at 69.87μg/cm~2,although the film had low anhydride content(3%) on the surface. 相似文献
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A film of α-naphthalene sulfonate-doped polypyrrole/poly(methylmethacrylate), PPy/α-NS−/PMMA, obtained from solution mixing was successfully used as sensing material for acetone vapor in lacquer with a high degree of selectivity based on electrical conductivity over acetic acid and a high degree of stability over the humidity change. Compared with pure PPy/α-NS−, the selectivity ratio of acetone/acetic acid response of PPy/α-NS−/PMMA blend with a PMMA/PPy weight ratio of 3.0 was ca. 3.9 times higher. The film was found to be insensitive to moisture unless the relative humidity (RH) was lower than 20% RH in which the selectivity ratio of acetone/acetic acid response was enhanced. The time required to reach the equilibrium for acetone exposure was found to increase slightly with increasing humidity. 相似文献
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Chaiseeda Kittichai Chantharadet Ladawan Chavasiri Warinthorn 《Research on Chemical Intermediates》2018,44(2):1305-1323
Research on Chemical Intermediates - Hexabromoacetone (HBA) was efficiently used for the protection of alcohols and aldehydes and deprotection of benzaldehyde dimethyl acetal, solketal, and other... 相似文献
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