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In this study, a facultative bacterium that converts fumarate to succinate at a high yield was isolated. The yield of biocon
version was enhanced about 1.2 times by addition of glucose into culture medium at an initial concentration of 6 g/L. When
the initial cell density was high (2 g/L), the succinate produced at pH 7.0 for initial fumarate concentrations of 30, 50,
80, and 100 g/L were 29.3, 40.9, 63.6, and 82.5 g/L, respectively, showing an increase with the initial fumarate concentration.
The high yield of 96.8%/mole of fumarate in just 4 h was obtained at the initial fumarate concentration of 30 g/L. Comparing
these values to those obtained with low cell culture (0.2 g/L), we found that the amount of succinate produced was similar,
but the production rate in the high cell culture was about three times higher than was the case in the low cell culture. This
strain converted fumarate to succinate at a rate of 3.5 g/L·h under the sparge of CO2. 相似文献
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Wee Young-Jung Yun Jong-Sun Kang Kui-Hyun Ryu Hwa-Won 《Applied biochemistry and biotechnology》2002,98(1-9):1093-1104
Enterococcus faecalis RKY1, a fumarate-reducing bacterium, was immobilized in an asymmetric hollow-fiber bioreactor (HFBR) for the continuous production
of succinic acid. The cells were inoculated into the shell side of the HFBR, which was operated in transverse mode. Since
the pH values in the HFBR declined during continuous operation to about 5.7, it was necessary to change the feed pH from 7.0
to 8.0 after 24 h of operation in order to enhance production of succinic acid. During continuous operation with a medium
containing fumarate and glycerol, the productivity of succinate was 3.0–10.9 g/(L·h) with an initial concentration of 30 g/L
of fumarate, 4.9–14.9 g/(L·h) with 50 g/L of fumarate, and 7.2–17.1 g/(L·h) with 80 g/L of fumarate for dilution rates between
0.1 and 0.4 h−1. The maximum productivity of succinate obtained by the HFBR (17.1 g of succinate /[L·h]) was 1.7 times higher than that of
the batch bioconversions (9.9 g of succinate /[L·h]) with 80 g/L of fumarate. Furthermore, the long-term stability of the
HFBR was demonstrated with a continuously efficient production of succinate for more than 15 d (360 h). 相似文献
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