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Vijaya D. Bharathi Kanbarpa Radharani Banda Jagadeesh Gajjela Ramulu Indu Bhushan Andra Naidu Ramesh Mullangi 《Chromatographia》2008,67(5-6):461-466
A rapid, sensitive, and simple HPLC–MS–MS method, with electro-spray ionization and cetirizine as internal standard (IS),
has been developed and validated for simultaneous quantification of fexofenadine and pseudoephedrine in human plasma. The
analytes were isolated from plasma by solid-phase extraction (SPE) on Oasis HLB cartridges. The compounds were chromatographed
on an RP 18 column with a mixture of ammonium acetate (10 mm, pH 6.4) and methanol as mobile phase. Quantification of the analytes was based on multiple reaction monitoring (MRM) of
precursor-to-product ion pairs m/z 502 → 466 for fexofenadine, m/z 166 → 148 for pseudoephedrine, and m/z 389 → 201 for cetirizine. The linear calibration range for both analytes was 2–1,700 ng mL−1 (r = 0.995), based on analysis of 0.1 mL plasma. Extraction recovery was 91.5 and 80.88% for fexofenadine and pseudoephedrine,
respectively. The method was suitable for analysis of human plasma samples obtained 72 h after administration of a drug containing
both fexofenadine and pseudoephedrine. 相似文献
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