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SL Mironov E Skorova G Taschenberger N Hartelt VO Nikolaev MJ Lohse S Kügler 《BMC neuroscience》2009,10(1):29-11
Background
cAMP is an ubiquitous second messenger mediating various neuronal functions, often as a consequence of increased intracellular Ca2+ levels. While imaging of calcium is commonly used in neuroscience applications, probing for cAMP levels has not yet been performed in living vertebrate neuronal tissue before. 相似文献2.
PF Hendrix SL Lachnicht MA Callaham X Zou 《Rapid communications in mass spectrometry : RCM》1999,13(13):1295-1299
Feeding strategies of earthworms and their influence on soil processes are often inferred from morphological, behavioral and physiological traits. We used (13)C and (15)N natural abundance in earthworms, soils and plants to explore patterns of resource utilization by different species of earthworms in three tropical ecosystems in Puerto Rico. In a high altitude dwarf forest, native earthworms Trigaster longissimus and Estherella sp. showed less (15)N enrichment ((15)N = 3-6 per thousand) than exotic Pontoscolex corethrurus ((15)N =7-9 per thousand) indicating different food sources or stronger isotopic discrimination by the latter. Conversely, in a lower altitude tabonuco forest, Estherella sp. and P. corethrurus overlapped completely in (15)N enrichment ((15)N = 6-9 per thousand), suggesting the potential for interspecific competition for N resources. A tabonuco forest converted to pasture contained only P. corethrurus which were less enriched in (15)N than those in the forest sites, but more highly enriched in (13)C suggesting assimilation of C from the predominant C(4) grass. These results support the utility of stable isotopes to delineate resource partitioning and potential competitive interactions among earthworm species. Copyright 1999 John Wiley & Sons, Ltd. 相似文献
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Kacinko SL Concheiro-Guisan M Shakleya DM Huestis MA 《Analytical and bioanalytical chemistry》2008,392(5):903-911
A liquid chromatography–tandem mass spectrometry method for the simultaneous quantification of buprenorphine (BUP), norbuprenorphine
(NBUP), buprenorphine glucuronide (BUP-Gluc), and norbuprenorphine glucuronide (NBUP-Gluc) in human urine was developed and
fully validated. Extensive endogenous and exogenous interferences were evaluated and limits of quantification were identified
empirically. Analytical ranges were 5–1,000 ng/mL for BUP and BUP-Gluc and 25–1,000 ng/mL for NBUP and NBUP-Gluc. Intra-assay
and interassay imprecision were less than 17% and recovery was 93–116%. Analytes were stable at room temperature, at 4 °C,
and for three freeze–thaw cycles. This accurate and precise assay has sufficient sensitivity and specificity for urine analysis
of specimens collected from individuals treated with BUP for opioid dependence. 相似文献
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