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Elemental fluorine and acetyl hypofluorite, labeled with 18F, were added to eight simple aryl-tin derivatives. Both reagents gave good radiochemical yields of labeled aryl fluorides. Overall, acetyl hypofluorite gave more consistent yields (~70%), while F2 gave more variable yields (54% – 95%).  相似文献   
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Research on Chemical Intermediates - An efficient, green, high yielding, and quick method for the synthesis of N-substituted decahydroacridine-1,8-diones was achieved by multicomponent reaction...  相似文献   
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We describe an optimised protocol for application of isobaric tags for relative and absolute quantitation (iTRAQ) and tandem mass spectrometry to obtain relative quantitative data from peptides derived from tryptic digestions of proteins fractionated by using the 2D liquid-phase ProteomeLab™ PF 2D technique. This methodology is suitable for the quantitation of proteins from a pool of co-eluting proteins which are often difficult to identify for the purpose of candidate protein selection for biologically relevant qualitative/quantitative changes under experimental conditions or in disease states. iTRAQ quantitation also facilitates the possibility of result to result comparison using other methodologies such as UV protein quantitation via the ProteomeLab™ PF 2D technique. The optimised protocol outlined here allows relative quantitation by MALDI-TOF/TOF mass spectrometry with high sensitivity and without the need to perform 2D HPLC separation of labelled peptides. The overall outcome is the simplification in the data complexity and the ease of use of the labelling protocol. This study is dedicated to Dr. Josef Chmelik in memory of his contribution and constant inspiration.  相似文献   
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