Metabolomic Profiling of Fresh Goji (Lycium barbarum L.) Berries from Two Cultivars Grown in Central Italy: A Multi-Methodological Approach

The metabolite profile of fresh Goji berries from two cultivars, namely Big Lifeberry (BL) and Sweet Lifeberry (SL), grown in the Lazio region (Central Italy) and harvested at two different periods, August and October, corresponding at the beginning and the end of the maturation, was characterized by means of nuclear magnetic resonance (NMR) and electrospray ionization Fourier transform ion cyclotron resonance (ESI FT-ICR MS) methodologies. Several classes of compounds such as sugars, amino acids, organic acids, fatty acids, polyphenols, and terpenes were identified and quantified in hydroalcoholic and organic Bligh-Dyer extracts. Sweet Lifeberry extracts were characterized by a higher content of sucrose with respect to the Big Lifeberry ones and high levels of amino acids (glycine, betaine, proline) were observed in SL berries harvested in October. Spectrophotometric analysis of chlorophylls and total carotenoids was also carried out, showing a decrease of carotenoids during the time. These results can be useful not only to valorize local products but also to suggest the best harvesting period to obtain a product with a chemical composition suitable for specific industrial use. Finally, preliminary studies regarding both the chemical characterization of Goji leaves generally considered a waste product, and the biological activity of Big Lifeberry berries extracts was also investigated. Goji leaves showed a chemical profile rich in healthy compounds (polyphenols, flavonoids, etc.) confirming their promising use in the supplements/nutraceutical/cosmetic field. MG63 cells treated with Big Lifeberry berries extracts showed a decrease of iNOS, COX-2, IL-6, and IL-8 expression indicating their significant biological activity.     

Chain Dominated Orders

We study finite partial orders which have a chain such that every element of the order either belongs to this chain or has all its covers in this chain. We show that such orders are exactly the orders being both interval orders and truncated lattices. We prove that their jump number is polynomially tractable and that their dimension is unbounded. We also show that every order admits a visibility model having such an order as host.     

Computing on-line the lattice of maximal antichains of posets

We consider the on-line computation of the lattice of maximal antichains of a finite poset . This on-line computation satisfies what we call the linear extension hypothesis: the new incoming vertex is always maximal in the current subposet of . In addition to its theoretical interest, this abstraction of the lattice of antichains of a poset has structural properties which give it interesting practical behavior. In particular, the lattice of maximal antichains may be useful for testing distributed computations, for which purpose the lattice of antichains is already widely used. Our on-line algorithm has a run time complexity of , where |P| is the number of elements of the poset, , |MA(P)| is the number of maximal antichains of and (P) is the width of . This is more efficient than the best off-line algorithms known so far.     

Towards the reconstruction of posets

The reconstruction conjecture for posets is the following: Every finite posetP of more than three elements is uniquely determined — up to isomorphism — by its collection of (unlabelled) one-element-deleted subposets P–{x}:xV(P).We show that disconnected posets, posets with a least (respectively, greatest) element, series decomposable posets, series-parallel posets and interval orders are reconstructible and that N-free orders are recognizable.We show that the following parameters are reconstructible: the number of minimal (respectively, maximal) elements, the level-structure, the ideal-size sequence of the maximal elements, the ideal-size (respectively, filter-size) sequence of any fixed level of the HASSE-diagram and the number of edges of the HASSE-diagram.This is considered to be a first step towards a proof of the reconstruction conjecture for posets.Research partly supported by DAAD.     

Infrared-Assisted Synthesis of Prebiotic Glycine

A novel approach has been developed to synthesize complex organic molecules (COMs) relevant to prebiotic chemistry, using infrared (IR) radiation to trigger the reaction. An original laboratory reactor working at low gas density and using IR irradiation was developed. In this way, glycine, the simplest brick of life, has been synthesized by assisting ion–molecule reaction with IR laser light. The ion-molecule complex constituted by acetic acid and hydroxylamine was formed in a mass spectrometer reactor and then irradiated with IR photons. As photoproducts, we obtained both glycine structures and some of its isomers. Anharmonic vibrational frequency calculations and fragmentation dynamics simulations allow for a better interpretation of the experimental data. This novel approach can be now extended to study other new synthetic pathways responsible for the formation of further COMs also with potential prebiotic relevance.     

Partial Orders on Weak Orders Convex Subsets

We study a visibility relation on the nonempty connected convex subsets of a finite partially ordered set and we investigate the partial orders representable as a visibility relation of such subsets of a weak order. Moreover, we consider restrictions where the subsets of the weak order are total orders or isomorphic total orders.     

Line directionality of orders

Some orders can be represented by translating convex figures in the plane. It is proved thatN-free and interval orders admit such representations with an unbounded number of directions. Weak orders, tree-like orders and two-dimensional orders of height one are shown to be two- directional. In all cases line segments can be used as convex sets.     

Secondary Metabolism Rearrangements in Linum usitatissimum L. after Biostimulation of Roots with COS Oligosaccharides from Fungal Cell Wall

In vitro culture of flax (Linum usitatissimum L.) was exposed to chitosan oligosaccharides (COS) in order to investigate the effects on the growth and secondary metabolites content in roots and shoots. COS are fragments of chitosan released from the fungal cell wall during plant–pathogen interactions. They can be perceived by the plant as pathogen-associated signals, mediating local and systemic innate immune responses. In the present study, we report a novel COS oligosaccharide fraction with a degree of polymerization (DP) range of 2–10, which was produced from fungal chitosan by a thermal degradation method and purified by an alcohol-precipitation process. COS was dissolved in hydroponic medium at two different concentrations (250 and 500 mg/L) and applied to the roots of growing flax seedlings. Our observations indicated that the growth of roots and shoots decreased markedly in COS-treated flax seedlings compared to the control. In addition, the results of a metabolomics analysis showed that COS treatment induced the accumulation of (neo)lignans locally at roots, flavones luteolin C-glycosides, and chlorogenic acid in systemic responses in the shoots of flax seedlings. These phenolic compounds have been previously reported to exhibit a strong antioxidant and antimicrobial activities. COS oligosaccharides, under the conditions applied in this study (high dose treatment with a much longer exposure time), can be used to indirectly trigger metabolic response modifications in planta, especially secondary metabolism, because during fungal pathogen attack, COS oligosaccharides are among the signals exchanged between the pathogen and host plant.     

ContributionsTree-visibility orders

We introduce a new class of partially ordered sets, called tree-visibility orders, containing interval orders, duals of generalized interval orders and height one orders. We give a characterization of tree-visibility orders by an infinite family of minimal forbidden suborders. Furthermore, we present an efficient recognition algorithm for tree-visibility orders.     

Inductive Characterizations of Finite Interval Orders and Semiorders

We introduce an inductive definition for two classes of orders. By simple proofs, we show that one corresponds to the interval orders class and that the other is exactly the semiorders class.