Preparation of '2,('3)-O-Acyl-pCpA derivatives as substrates for T4 RNA ligase-mediated “chemical aminoacylation”

The synthesis of '2('3)-O-(amino)acyl-pCpA derivatives is described and the products are characterized chemically and as substrates for T4 RNA ligase in the presence of E. coli tRNA ^phe-C_OH. The modified (N-acetylammo)acyl-tRNA's so derived are characterized by their chromatographic and biochemical properties, and by their ability to act as P-site tRNA donors in the peptidyltransferase reaction.     

The quantitative determination of uranyl ion by means of the uranyl-sensitised photodecomposition of oxalic acid

The uranyl-sensitised photodecomposition of oxalic acid is followed by the use of a modified Warburg constant-volume micro-manometric apparatus. The minimum concentration of uranyl ion determined was 100 μM.