Growth inhibition in animal cell culture

Eight independent cell lines accumulated ammonia in culture to concentrations between 1.3 and 2.9 mM. The growth inhibition of such concentrations of ammonium chloride when added to culture medium was variable. The cell lines tested could be divided into 3 groups depending on their growth response to 2 mM added NH4Cl. In the first group (293, HDF, Vero, and PQXB1/2) little (less than 14%) or no growth inhibition occurred. In the second group (McCoy and MDCK) a reduction in final cell yield of 50-60% was observed. The third group (HeLa and BHK) was most sensitive to the effects of NH4Cl with growth inhibition (greater than 75%) compared to controls. The growth inhibitory effect of added lactate up to 20 mM was negligible (less than 10%) for 3 cell lines, although one cell line (PQXB1/2) showed greater sensitivity. The interactive effects of ammonia and lactate were determined in a matrix experiment. At lactate (greater than 12 mM) and ammonia (1-4 mM), the growth inhibitory effects of the two components were synergistic. However, at low concentrations of lactate (less than 12 mM) the toxic effect of ammonia was reduced. A proposed mechanism for the sparing effect of lactate on ammonia toxicity is discussed. This may have importance in developing strategies for the optimal growth of ammonia-sensitive cell lines.     

Polyphenolics with Strong Antioxidant Activity from Acacia nilotica Ameliorate Some Biochemical Signs of Arsenic-Induced Neurotoxicity and Oxidative Stress in Mice

Neurotoxicity is a serious health problem of patients chronically exposed to arsenic. There is no specific treatment of this problem. Oxidative stress has been implicated in the pathological process of neurotoxicity. Polyphenolics have proven antioxidant activity, thereby offering protection against oxidative stress. In this study, we have isolated the polyphenolics from Acacia nilotica and investigated its effect against arsenic-induced neurotoxicity and oxidative stress in mice. Acacia nilotica polyphenolics prepared from column chromatography of the crude methanol extract using diaion resin contained a phenolic content of 452.185 ± 7.879 mg gallic acid equivalent/gm of sample and flavonoid content of 200.075 ± 0.755 mg catechin equivalent/gm of sample. The polyphenolics exhibited potent antioxidant activity with respect to free radical scavenging ability, total antioxidant activity and inhibition of lipid peroxidation. Administration of arsenic in mice showed a reduction of acetylcholinesterase activity in the brain which was counteracted by Acacia nilotica polyphenolics. Similarly, elevation of lipid peroxidation and depletion of glutathione in the brain of mice was effectively restored to normal level by Acacia nilotica polyphenolics. Gallic acid methyl ester, catechin and catechin-7-gallate were identified in the polyphenolics as the major active compounds. These results suggest that Acacia nilotica polyphenolics due to its strong antioxidant potential might be effective in the management of arsenic induced neurotoxicity.     

Scattering matrices for the quantum body problem

Let be a generalized body Schrödinger operator with very short range potentials. Using Melrose's scattering calculus, it is shown that the free channel `geometric' scattering matrix, defined via asymptotic expansions of generalized eigenfunctions of , coincides (up to normalization) with the free channel `analytic' scattering matrix defined via wave operators. Along the way, it is shown that the free channel generalized eigenfunctions of Herbst-Skibsted and Jensen-Kitada coincide with the plane waves constructed by Hassell and Vasy and if the potentials are very short range.

     

Charge inversion via concurrent cation and anion transfer: application to corticosteroids

A novel charge inversion process that involves the removal of an excess cation from an analyte ion and the transfer of an anion to the neutral analyte in a single ion/ion encounter is described. Polyamidoamine (PAMAM) half-generation dendrimer anions that contain small anions, such as the chloride ion, were used as charge inversion reagents. Several competing processes can occur that include removal of the cation to neutralize the analyte, the removal of the excess cation and an additional proton to yield the deprotonated molecule, or removal of the excess cation and transfer of a small anion to the analyte. For the latter process to dominate, several requirements for both the reagent anion and the analyte cation must be met. The reagent anion must form multiply charged anions and must be able to incorporate one or more small anions for transfer. The analyte must have no strongly acidic sites as well as a relatively high affinity for small anion attachment. The PAMAM dendrimer anions must meet the conditions for the reagent anions and the cations of the corticosteroids meet the conditions for the analyte. The estrogenic steroid estrone, on the other hand, does not meet the requirements and, as a result, is largely neutralized when reacted with the reagent anions. This reaction, therefore, is highly selective and might serve as a useful reaction for the screening of appropriate analytes.     

Eigenvalues of Schrödinger operators with potential asymptotically homogeneous of degree

We strengthen and generalise a result of Kirsch and Simon on the behaviour of the function , the number of bound states of the operator in below . Here is a bounded potential behaving asymptotically like where is a function on the sphere. It is well known that the eigenvalues of such an operator are all nonpositive, and accumulate only at 0. If the operator on the sphere has negative eigenvalues less than , we prove that may be estimated as

Thus, in particular, if there are no such negative eigenvalues, then has a finite discrete spectrum. Moreover, under some additional assumptions including the fact that and that there is exactly one eigenvalue less than , with all others , we show that the negative spectrum is asymptotic to a geometric progression with ratio .

     

The spectral projections and the resolvent for scattering metrics

In this paper, we consider a compact manifold with boundaryX equipped with a scattering metricg as defined by Melrose [9]. That is,g is a Riemannian metric in the interior ofX that can be brought to the formg=x ⁻⁴ dx²+x^{−2 h’} near the boundary, wherex is a boundary defining function andh’ is a smooth symmetric 2-cotensor which restricts to a metrich on ϖX. LetH=Δ+V, whereV∈x ²C^∞ (X) is real, soV is a ‘short-range’ perturbation of Δ. Melrose and Zworski started a detailed analysis of various operators associated toH in [11] and showed that the scattering matrix ofH is a Fourier integral operator associated to the geodesic flow ofh on ϖX at distance π and that the kernel of the Poisson operator is a Legendre distribution onX×ϖX associated to an intersecting pair with conic points. In this paper, we describe the kernel of the spectral projections and the resolvent,R(σ±i0), on the positive real axis. We define a class of Legendre distributions on certain types of manifolds with corners and show that the kernel of the spectral projection is a Legendre distribution associated to a conic pair on the b-stretched productX _b ² (the blowup ofX ² about the corner, (ϖX)²). The structure of the resolvent is only slightly more complicated. As applications of our results, we show that there are ‘distorted Fourier transforms’ forH, i.e., unitary operators which intertwineH with a multiplication operator and determine the scattering matrix; we also give a scattering wavefront set estimate for the resolventR(σ±i0) applied to a distributionf.     

Transfer measurements for the Ti+Ni systems at near barrier energies

Large enhancements have been observed in the sub-barrier fusion cross sections for Ti+Ni systems in our previous studies. Coupled channel calculations incorporating couplings to 2⁺ and 3⁻ states failed to explain these enhancements completely. A possibilty of transfer channels contributing to the residual enhancements had been suggested. In order to investigate the role of relevant transfer channels, measurements of one- and two-nucleon transfer were carried out for ^46,48Ti+⁶¹Ni systems. The present paper gives the results of these studies.     

Strichartz Estimates Without Loss on Manifolds with Hyperbolic Trapped Geodesics

In [Do], Doi proved that the ${L^{2}_{t}H^{1/2}_{x}}In [Do], Doi proved that the L²_tH^1/2_x{L^{2}_{t}H^{1/2}_{x}} local smoothing effect for Schr?dinger equations on a Riemannian manifold does not hold if the geodesic flow has one trapped trajectory. We show in contrast that Strichartz estimates and L ¹ → L ^∞ dispersive estimates still hold without loss for e ^itΔ in various situations where the trapped set is hyperbolic and of sufficiently small fractal dimension.     

Microwave-assisted synthesis of 3-aryl-pyrimido[5,4-e][1,2,4]triazine-5,7(1H,6H)-dione libraries: derivatives of toxoflavin

The parallel synthesis of a library of toxoflavin derivatives is described. The microwave-assisted approach involves the de novo generation of the heterocyclic scaffold and allows for facile introduction of a variety of fragments.     

A host-guest optical sensor for aliphatic amines based on lipophilic cyclodextrin

A host-guest optical sensor for the determination of aliphatic amines as exemplified by octylamine is proposed. It is based on the reversible fluorescence enhancement of heptakis(2,6-di-O-isobutyl)-β-cyclodextrin(DOB-β-CD) hosting tetraphenylporphyrin (TPP) immobilized in poly(vinyl chloride) (PVC) membrane by aliphatic amine extracted from aqueous phase into membrane phase. The optimum membrane contained 1.15 wt % TPP, 6.15 wt % DOB-β-CD as sensing reagent and other membrane materials. The fluorescence enhancement of the membrane resulted from the formation of a stable three-component complex among DOB-β-CD, TPP, and aliphatic amines. With the optimum conditions described, the fluorescence response of the sensor to octylamine shows a good correlation with the theoretically derived equation in the range 1.0 × 10^–6 to 8.0 × 10^–4 mol/L. The response characteristics including reversibility, response time, reproducibility and lifetime and selectivity of this optical device are also discussed in detail. This sensor has also been applied for the determination of octylamine in water samples containing interferents with satisfactory recovery.