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Back Cover: Yoctoliter Thermometry for Single‐Molecule Investigations: A Generic Bead‐on‐a‐Tip Temperature‐Control Module (Angew. Chem. Int. Ed. 13/2014) 下载免费PDF全文
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2010年高考物理计算题突出考查了学生对物理综合问题的分析能力,尤其是对物理过程的考查,更是常考常新,非常精彩.这些物理试题具有普遍性与广泛性,在解题时要求考生有扎实的基础知识和良好的解决问题的思维程序,构建理想化的过程模型. 相似文献
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Dissecting Cooperative Communications in a Protein with a High‐Throughput Single‐Molecule Scalpel 下载免费PDF全文
Miscued communication often leads to misfolding and aggregation of the proteins involved in many diseases. Owing to the ensemble average property of conventional techniques, detailed communication diagrams are difficult to obtain. Mechanical unfolding affords an unprecedented perspective on cooperative transitions by observing a protein along a trajectory defined by two mutated cysteine residues. Nevertheless, this approach requires tedious sample preparation at the risk of altering native protein conformations. To address these issues, we applied click chemistry to tether a protein to the two dsDNA handles through primary amines in lysine residues as well as at the N terminus. As a proof of concept, we used laser tweezers to mechanically unfold and refold calmodulin along 36 trajectories, maximally allowed by this strategy in a single batch of protein preparation. Without a priori knowledge of the particular residues to which the double‐stranded DNA handles attach, we used hierarchical cluster analysis to identify 20 major trajectories, according to the size and the pattern of unfolding transitions. We dissected the cooperativity into all‐or‐none and partially cooperative events, which represent strong and weak high‐order interactions in proteins, respectively. Although the overall cooperativity is higher within the N or C lobe than that between the lobes, the all‐or‐none cooperativity is anisotropic among different the unfolding trajectories and becomes relatively more predominant when the size of the protein segments increases. The average cooperativity for all‐or‐none transitions falls within the expected range observed by ensemble techniques, which supports the hypothesis that unfolding of a free protein can be reconstituted from individual trajectories. 相似文献
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主要介绍自由电子激光相干强太赫兹源(FEL-THz)装置上的砷化镓光阴极直流高压注入器的研究进展,并讨论其驱动未来高重复频率短波长自由电子激光器的差距。通过综合砷化镓阴极寿命的三大影响因素,提出了其工作寿命的定性物理模型;通过该模型对阴极和注入器进行优化,在直流高压电子枪上得到了5mA,32min的连续稳定输出;测量了电子束在4.8mA下归一化发射度约为4.0πmm·mrad,阴极热发射度约为0.6πmm·mrad,电子束本征横向能量约为92meV,250keV电子束在距离阴极90.6cm处纵向均方根长度约为11.5ps。这一束流状态已经基本满足FEL-THz需求。 相似文献
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1文字说明详略得当、言简意赅
文字说明能反映解题思路,展示思维过程.写文字说明时,应根据题意,弄清哪些文字必要,需要写,哪些不必要,可以忽略,做到详略得当,言简意赅。必要的文字说明,有以下几项: 相似文献
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ALD-clad-pumped nanosecond Tm3+ doped silica fiber amplifier was reported that provided up to a 2-W average power output with a slope efficiency of 48.6% with respect to the absorbed pumped power. The input signal central wavelength was at 1.99 μm, with a 119.5-ns pulse duration and 5-kHz repeat rates. The pump sources were a 793-nm LD output with a 400-μm pigtail fiber. The gain fiber was D-shaped Tm3+ doped silica double-clad fiber with a diameter of 27.5/400 μm. The output amplified pulse and spectrum were in accordance with the input signal. This is the first report to the authors’ knowledge on the general experimental investigation of a LD-clad-pumped Tm3+ doped silica nanosecond-fiber amplifier at 1.99 μm. 相似文献
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Changpeng Hu Sagun Jonchhe Pravin Pokhrel Deepak Karna Hanbin Mao 《Chemical science》2021,12(30):10159
Mechanical unfolding of biomolecular structures has been exclusively performed at the single-molecule level by single-molecule force spectroscopy (SMFS) techniques. Here we transformed sophisticated mechanical investigations on individual molecules into a simple platform suitable for molecular ensembles. By using shear flow inside a homogenizer tip, DNA secondary structures such as i-motifs are unfolded by shear force up to 50 pN at a 77 796 s−1 shear rate. We found that the larger the molecules, the higher the exerted shear forces. This shear force approach revealed affinity between ligands and i-motif structures. It also demonstrated a mechano-click reaction in which a Cu(i) catalyzed azide–alkyne cycloaddition was modulated by shear force. We anticipate that this ensemble force spectroscopy method can investigate intra- and inter-molecular interactions with the throughput, accuracy, and robustness unparalleled to those of SMFS methods.Shear force in a homogenizer mechanically unfolds an ensemble set of biomolecular structures. 相似文献