排序方式: 共有1条查询结果,搜索用时 0 毫秒
1
1.
Sode Koji Sugiura Hiroto Tsugawa Wakako Watazu Yoshihumi Hamafuji Tetsuro 《Applied biochemistry and biotechnology》2000,84(1-9):947-954
A novel enzymatic organic synthesis was reported, utilizing glucose-3-dehydrogenase (G3DH) and its regeneration via electrochemical
methods. We combined the water-soluble G3DH prepared from a marine bacterium, Halomonas sp. α-15, and electron mediator with the electrode system in order to regenerate the enzyme. Using this system, the conversion
of 1,5-anhydro-d-glucitol (1,5AG), a diabetes marker in human blood, was investigated. The final yield of the product, 3-keto anhydroglucitol
(3-ketoAG), which was identified by 13C nuclear magnetic resonance, was 82% based on the initial amount of 1,5AG. The electrochemical yield of the reaction proceeded
almost stoichiometrically. The electrochemical conversion rate of 1,5AG was 1.24 mmol/(L·h), and the electrochemical yield
of 1,5AG consumption was 80%, whereas that for 3-ketoAG was 60%. 相似文献
1