C-terminal processing of yeast Spt7 occurs in the absence of functional SAGA complex

Background  

Spt7 is an integral component of the multi-subunit SAGA complex that is required for the expression of ~10% of yeast genes. Two forms of Spt7 have been identified, the second of which is truncated at its C-terminus and found in the SAGA-like (SLIK) complex.     

Product inhibition in packed-bed immobilized enzyme reactors for complex processes: Modelling of two-substrate processes

An analytical model was developed for describing the performance of packed-bed enzymic reactors operating with two cosubstrates, and when one of the reaction products is inhibitory to the enzyme. To this aim, the compartmental analysis technique was used. The relevant equations obtained were solved numerically, and the effect of the main operational parameters on the reactor characteristics were studied.Notation C _infa,i ^sup* local concentration of products in the pores of stage i - C _j,i concentration of substrate j in the pores of stage i - D _infa ^sup* internal (pore) diffusion coefficient for the reaction product a - D _j internal (pore) diffusion coefficient of substrate j - J _infa,i ^sup* net flux of product a, taking place from the pores of stage i into the corresponding bulk phase - J _j,i net flux of substrate j, taking place from the bulk phase of stage i into the corresponding pores - K _b inhibition constant - K _m,1, K _m,2 Michaelis constants for substrate 1 and 2, respectively - K _q inhibition constant - n total number of elementary stages in the reactor - Q volumetric flow rate throughout the reactor - R _j,i, R _infa,i ^sup* local reaction rates in pores of stage i, in terms of concentration of substrate j and product a respectively - S _infa,i ^sup* , S _infa,i-1 ^sup* bulk concentration of the reaction product a, in the stages i and i — 1, respectively - S _j,0 concentration of substrate j in the reactor feed - S _j,i-1, S _j,i concentration of substrate j in the bulk phase leaving stages i — 1 and i, respectively - V total volume of the reactor - V _m maximal reaction rate in terms of volumetric units - y axial coordinate of the pores - y ₀ depth of the pores - ^* dimensionless parameter, defined in Equation (22) - ₁ dimensionless parameter, defined in Equation (6) - ₂ dimensionless parameter, defined in Equation (6) - ₁ dimensionless parameter, defined in Equation (6) - ₂ dimensionless parameter, defined in Equation (6) - ^* dimensionless parameter, defined in Equation (22) - ₁ dimensionless parameter, defined in Equation (6) - ₂ dimensionless parameter, defined in Equation (6) - ^* dimensionless parameter, defined in Equation (22) - ^* dimensionless parameter, defined in Equation (22) - volumetric packing density of catalytic particles (dimensionless) - porosity of the catalytic particles (dimensionless) - V _infi ^sup* dimensionless concentration of reaction product in pores of stage i, defined in Equation (17) - j,i dimensionless concentration of substrate j in pores of stage i; defined in Equation (6) - _j,i-1, _j.i dimensionless concentration of substrate j in the bulk phase of stage i; defined in Equation (6) - dimensionless position along the pore; defined in Equation (6)     

Topological differential fields

We consider first-order theories of topological fields admitting a model-completion and their expansion to differential fields (requiring no interaction between the derivation and the other primitives of the language). We give a criterion under which the expansion still admits a model-completion which we axiomatize. It generalizes previous results due to M. Singer for ordered differential fields and of C. Michaux for valued differential fields. As a corollary, we show a transfer result for the NIP property. We also give a geometrical axiomatization of that model-completion. Then, for certain differential valued fields, we extend the positive answer of Hilbert’s seventeenth problem and we prove an Ax-Kochen-Ershov theorem. Similarly, we consider first-order theories of topological fields admitting a model-companion and their expansion to differential fields, and under a similar criterion as before, we show that the expansion still admits a model-companion. This last result can be compared with those of M. Tressl: on one hand we are only dealing with a single derivation whereas he is dealing with several, on the other hand we are not restricting ourselves to definable expansions of the ring language, taking advantage of our topological context. We apply our results to fields endowed with several valuations (respectively several orders).     

(E)-2-Benzylidenebenzocyclanones, part VIII: spectrophotometric determination of pKa values of some natural and synthetic chalcones and their cyclic analogues

Abstract  

UV–Vis spectrophotometry was used to determine acid dissociation constant (pK _a) values of the natural flavonoids phloretin, phlorizin, naringenin, and naringin, as well as 4′-hydroxychalcone, 4′-(dimethylamino)chalcone, and their cyclic analogues. Comparison of the results with those previously reported for the natural flavonoids showed the applied method is a relatively straightforward and easy-to-perform technique for the determination of pK _a values of compounds with relatively low solubility. Comparative analysis of the pK _a values of the synthetic chalcones showed a strong correlation between the degree of conjugation and the acid strength of the respective compounds with different geometry. Our results provide further evidence that modification of the three-dimensional structure of open-chain bioactive compounds is the method of choice to modify not only their stereochemistry but also their physicochemical properties.     

Note sur les corps différentiellement clos valués

In the paper by Guzy and Point, Differential topological fields, the model-completion ${(\mathit{OVF})}_D^1$ of the theory of ordered valued differential fields ${\mathit{OVF}}_D$ is established. Models of this theory are closed ordered differential fields (the theory CODF was studied by Singer) which have a non-trivial convex (for the order) subring as valuation ring. Here we prove the valued analogue of a result of Singer: if K is a model of ${(\mathit{OVF})}_D^1$ then $K(\mathrm{i})$ (${\mathrm{i}}^2=?1$) is a model of the theory of differentially closed valued fields which is the model-completion of the theory of non-trivially valued differential fields of characteristic zero. To cite this article: N. Guzy, C. R. Acad. Sci. Paris, Ser. I 341 (2005).