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1.
A method using capillary gel electrophoresis with laser-induced fluorescence detection is described which permits complete sequence determination of antisense DNA analogues of unknown sequence. This method, originally created as a tool to confirm the sequence of antisense oligonucleotides being developed as therapeutic drugs, utilizes data collected under a range of experimental conditions described by the Ogston model as applied to gel electrophoresis. A linear relationship independent of experimental conditions between the relative electrophoretic migration time and the oligonucleotide base number was observed and is shown to be consistent with a simplified version of this model and can be used to facilitate the sequence determination. 相似文献
2.
A comparison of steady-state evoked potentials to modulated tones in awake and sleeping humans. 总被引:12,自引:0,他引:12
L T Cohen F W Rickards G M Clark 《The Journal of the Acoustical Society of America》1991,90(5):2467-2479
Steady-state evoked potential responses were measured to binaural amplitude-modulated (AM) and combined amplitude- and frequency-modulated (AM/FM) tones. For awake subjects, AM/FM tones produced larger amplitude responses than did AM tones. Awake and sleeping responses to 30-dB HL AM/FM tones were compared. Response amplitudes were lower during sleep and the extent to which they differed from awake amplitudes was dependent on both carrier and modulation frequencies. Background EEG noise at the stimulus modulation frequency was also reduced during sleep and varied with modulation frequency. A detection efficiency function was used to indicate the modulation frequencies likely to be most suitable for electrical estimation of behavioral threshold. In awake subjects, for all carrier frequencies tested, detection efficiency was highest at a modulation frequency of 45 Hz. In sleeping subjects, the modulation frequency regions of highest efficiency varied with carrier frequency. For carrier frequencies of 250 Hz, 500 Hz, and 1 kHz, the highest efficiencies were found in two modulation frequency regions centered on 45 and 90 Hz. For 2 and 4 kHz, the highest efficiencies were at modulation frequencies above 70 Hz. Sleep stage affected both response amplitude and background EEG noise in a manner that depended on modulation frequency. The results of this study suggest that, for sleeping subjects, modulation frequencies above 70 Hz may be best when using steady-state potentials for hearing threshold estimation. 相似文献
3.
Based on computer simulations, Kauffman (Physica D, 10, 145-156, 1984) made several generalizations about a random Boolean cellular automaton which he invented as a model of cellular metabolism. Here we give the first rigorous proofs of two of Kauffman's generalizations: a large fraction of vertices stabilize quickly, consequently the length of cycles in the automaton's behavior is small compared to that of a random mapping with the same number of states; and reversal of the states of a large fraction of the vertices does not affect the cycle to which the automaton moves. 相似文献
4.
Finite groups acting on rings by automorphisms, and group-graded rings are instances of Hopf algebras H acting on H-module algebras A. We study such actions. Let AH = {a ε A¦h · A = (h) a, all h ε H}, the ring of H-invariants, and form the smash product A # H. We study the ring extensions AH A A # H. We prove a Maschke-type theorem for A # H-modules. We form an associated Morita context [AH, A, A, A # H] and use these to get connections between the various rings. 相似文献
5.
Summary Excessive breakdown of elastin, a structural protein, may be related to aortic disease and emphysema. Since L-valyl-L-proline occurs in high concentrations in elastin, a rapid and sensitive method using HPLC with post-column on-line derivatization was used to measure the dipeptide from swine aortic tissue, and the amount of elastin present was determined. Elastin was extracted by alkaline hydrolysis. After neutralization and filtration, the sample was injected onto a ODS-2 gel column, and the dipeptide was eluted by a linear gradient of 0 to 10% of 1-propanol in 50 mM heptafluorobutyrate, pH 3, at a flow rate of 1 ml/min. The eluent was reacted with fluorescamine at pH 8.6, and fluorescence was detected at an excitation wavelength of 395 nm and a 455 nm cutoff emission filter.Presented at the 17th International Symposium on Chromatography, September 25–30, 1988, Vienna, Austria. 相似文献
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Guy Bayada 《Journal of Mathematical Analysis and Applications》2003,282(1):212-231
The asymptotic behaviour of a Stokes flow with Tresca free boundary friction conditions when one dimension of the fluid domain tends to zero is studied. A specific Reynolds equation associated with variational inequalities is obtained and uniqueness is proved. 相似文献
9.
Daniel C. Cohen Peter Orlik 《Transactions of the American Mathematical Society》2005,357(8):3031-3050
We study the Gauss-Manin connection for the moduli space of an arrangement of complex hyperplanes in the cohomology of a complex rank one local system. We define formal Gauss-Manin connection matrices in the Aomoto complex and prove that, for all arrangements and all local systems, these formal connection matrices specialize to Gauss-Manin connection matrices.
10.
In the nineteenth century, field theory brilliantly resolveda number of questions that had taxed mathematicians for centuries;for example, The circle cannot be squared by straightedge and compass, and solving polynomial equations by radicalsis not always possible. These successes have continued to beheld up as superb examples of the power of mathematical thought,and are demonstrated at an undergraduate level. The purposeof this article is to provide another such natural example whichleads to a concrete realisation of the free group on 2 generators. 相似文献