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Worden R. M. Grethlein A. J. Zeikus J. G. Datta R. 《Applied biochemistry and biotechnology》1989,20(1):687-698
Applied Biochemistry and Biotechnology - Carbon monoxide is produced in high concentrations by gasification of coal or biomass, and is a potentially inexpensive feedstock for biological processes.... 相似文献
2.
Converse A. O. Kwarteng I. K. Grethlein H. E. Ooshima H. 《Applied biochemistry and biotechnology》1989,(1):63-78
The results of an experimental study of the acid hydrolysis of hardwood are presented in the form of values for the three
parameters, activation energy, power on the acid concentration, and pre-exponen-tial factor, of the first order kinetic constants
for each of the following reaction participants: xylan remaining, glucan remaining, xylose formed, and xylose decomposed.
These are used as a base for a quantitative theory to predict the temperature, time, and acid concentrations needed for effective
pretreatment of the substrate for subsequent enzymatic hydrolysis of the glucan. This theory is based on the assumption that
successful pretreatment requires >90% removal of the xylan, <10% removal of the glucan, and >80% xylose yield. This theory
is compared with selected published data. 相似文献
3.
Grethlein A. J. Worden R. M. Jain M. K. Datta R. 《Applied biochemistry and biotechnology》1990,(1):875-884
Continuous, steady-state fermentations using carbon monoxide gas as the sole carbon and energy source have been achieved with
the CO strain ofButyribacterium methylotrophicum. Fermentation pH was found to regulate carbon monoxide metabolism over the pH range of 6.8 to 5.0. Cell growth diminished
at low pH, with washout occurring at pH 5.0. As observed previously in batch culture, lower pH values favored production of
butyrate over acetate. The mechanism responsible for this trend is currently being investigated by quantification of key intracellular
enzyme activities.
At low pH values, direct, steady-state fermentation of carbon monoxide to alcohols has been verified. Of major significance
is the production of butanol from carbon monoxide in pure culture. This newly identified pathway provides a potential mechanism
for direct bioconversion of synthesis gas to butanol. 相似文献
4.
Torres José L. Grethlein Hans E. Lynd Lee R. 《Applied biochemistry and biotechnology》1989,(1):621-633
High energy costs are associated with the recovery of ethanol from fermentation broths. This paper discusses a computer simulation
of the Dartmouth Process, which aims to reduce these costs by the use of IHOSR distillation, extensive heat integration, and
extractive distillation using a salt.
To resolve the uncertainty in modeling alcohol-water-salt vapor-liquid equilibrium, a new and more accurate activity coefficient
model was used. An Aspen™ model was used to generate capital and energy costs for a range of ethanol concentrations in the
feed. Simulation results show that the Dartmouth Process offers substantial economic advantages over benzene azeotropic distillation,
particularly at low feed concentrations. 相似文献
5.
Thompson David N. Hames Bonnie R. Reddy C. A. Grethlein Hans E. 《Applied biochemistry and biotechnology》1998,(1):967-982
The abilities of lignin peroxidase (LIP) and manganese peroxidase (MNP) fromPhanerochaete chrysosporium to degrade an insoluble hardwood lignin in vitro in aqueous media were tested. Neither LIP nor MNP appreciably changed the
mass or lignin content, although both produced small amounts of unique solubilized lignin fragments. Treatment with both LIP
and MNP, however, decreased the mass by 11%, decreased the lignin content by 5.1% (4.2% as total weight), and solubilized
unique lignin-derived molecules. These results suggest that LIP and MNP synergistically degrade high molecular weight insoluble
lignin, but singly, neither enzyme is sufficient to effect lignin degradation. 相似文献
6.
Grethlein A. J. Soni B. K. Worden R. M. Jain M. K. 《Applied biochemistry and biotechnology》1992,34(1):233-246
Applied Biochemistry and Biotechnology - Studies were conducted to determine the effect of hydrogen sulfide (H2S) on the growth and metabolism ofButyribacterium methylotrophicum andClostridium... 相似文献
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