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Applied Biochemistry and Biotechnology - 相似文献
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Glassner M Kempe K Schubert US Hoogenboom R Barner-Kowollik C 《Chemical communications (Cambridge, England)》2011,47(38):10620-10622
An efficient method for the preparation of cyclopentadienyl endcapped poly(2-ethyl-2-oxazoline) (PEtOx-Cp) via cationic ring-opening polymerization utilizing sodium cyclopentadienide as a termination agent is presented. Subsequent Diels-Alder reactions with N-substituted maleimides proceed quantitatively at ambient temperature. A block copolymer (PEtOx-b-PEG) is prepared employing maleimide terminated poly(ethylene glycol). 相似文献
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Li Q Deka C Glassner BJ Arnold K Li-Sucholeiki XC Tomita-Mitchell A Thilly WG Karger BL 《Journal of separation science》2005,28(12):1375-1389
A fundamental goal ingenomics is the discovery of genetic variation that contributes to disease states or to differential drug responses. Single nucleotide polymorphism (SNP) detection has been the focus of much attention in the study of genetic variation over the last decade. These SNPs typically occur at a frequency greater than 1% in the human genome. Recently, low-frequency alleles are also being increasingly recognized as critical to obtain an improved understanding of the correlation between genetic variation and disease. Although many methods have been reported for the discovery and scoringof SNPs, sensitive, automated, and cost-effective methods and platforms for the discovery of low-frequency alleles are not yet readily available. We describe here an automated multicapillary instrument for high-throughput detection of low-frequency alleles from pooled samples using constant denaturant capillary electrophoresis. The instrument features high optical sensitivity (1 x 10(-12) M fluorescein detection limit), precise and stable temperature control (+/- 0.01degrees C), and automation for sample delivery, injection, matrix replacement, and fraction collection. The capillary array is divided into six groups of four capillaries, each of which can be independently set at any temperature ranging from room temperature to 90 degrees C. The key performance characteristics of the instrument are reported. 相似文献
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Fritz Glassner 《Monatshefte für Chemie / Chemical Monthly》1907,28(3):281-295
Ohne ZusammenfassungZum Schlusse sei es mir noch gestattet, meinem hochverehrten Lehrer Herrn Prof. Dr. W. Suida für seine Anregungen und erteilten Ratschläge meinen ergebensten Dank auszusprechen. 相似文献
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Glassner M Delaittre G Kaupp M Blinco JP Barner-Kowollik C 《Journal of the American Chemical Society》2012,134(17):7274-7277
Tailor-made water-soluble macromolecules, including a glycopolymer, obtained by living/controlled RAFT-mediated polymerization are demonstrated to react in water with diene-functionalized poly(ethylene glycol)s without pre- or post-functionalization steps or the need for a catalyst at ambient temperature. As previously observed in organic solvents, hetero-Diels-Alder (HDA) conjugations reached quantitative conversion within minutes when cyclopentadienyl moieties were involved. However, while catalysts and elevated temperatures were previously necessary for open-chain diene conjugation, additive-free HDA cycloadditions occur in water within a few hours at ambient temperature. Experimental evidence for efficient conjugations is provided via unambiguous ESI-MS, UV/vis, NMR, and SEC data. 相似文献
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Dr. Ozcan Altintas Dr. Mathias Glassner Dr. Cesar Rodriguez‐Emmenegger Dr. Alexander Welle Vanessa Trouillet Prof. Dr. Christopher Barner‐Kowollik 《Angewandte Chemie (International ed. in English)》2015,54(19):5777-5783
The efficient trapping of photogenerated thioaldehydes with functional shelf‐stable nitrile oxides in a 1,3‐dipolar cycloaddition is a novel and versatile photochemical strategy for polymer end‐group functionalization and surface modification under mild and equimolar conditions. The modular ligation in solution was followed in detail by electrospray ionization mass spectrometry (ESI‐MS). X‐ray photoelectron spectroscopy (XPS) was employed to analyze the functionalized surfaces, whereas time‐of‐flight secondary‐ion mass spectrometry (ToF‐SIMS) confirmed the spatial control of the surface functionalization using a micropatterned shadow mask. Polymer brushes were grown from the surface in a spatially confined regime by surface‐initiated atom transfer radical polymerization (SI‐ATRP) as confirmed by TOF‐SIMS, XPS as well as ellipsometry. 相似文献