排序方式: 共有1条查询结果,搜索用时 0 毫秒
1
1.
Ghiulendan Aptisa Fernando Benavente Victoria Sanz-Nebot Elisabeta Chirila José Barbosa 《Analytical and bioanalytical chemistry》2010,396(4):1571-1579
Modelling electrophoretic mobility as a function of pH can be simultaneously used for determination of ionization constants
and for rapid selection of the optimum pH for separation of mixtures of the modelled compounds. In this work, equations describing
the effect of pH on electrophoretic behaviour were used to investigate migration of a series of polyprotic amphoteric peptide
hormones between pH 2 and 12 in polybrene-coated capillaries. Polybrene (hexadimethrin bromide) is a polymer composed of quaternary
amines that is strongly adsorbed by the fused-silica inner surface, preventing undesired interactions between the peptides
and the inner capillary wall. In polybrene-coated capillaries the separation voltage must be reversed, because of the anodic
electroosmotic flow promoted by the polycationic polymer attached to the inner capillary wall. The possibility of using polybrene-coated
capillaries for determination of accurate ionization constants has been evaluated and the optimum pH for separation of a mixture
of the peptide hormones studied has been selected. Advantages and disadvantages of using bare fused-silica and polybrene-coated
capillaries for these purposes are discussed. 相似文献
1